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anti-Human DFFA antibody for Immunofluorescence

Recommended DFFA Antibody (supplied by: Log in to see )

Antigen
DNA Fragmentation Factor, 45kDa, alpha Polypeptide (DFFA) Antibodies
  • A330085O09Rik
  • DFF-45
  • DFF1
  • DFF35
  • Dff45
  • DFFA
  • ICAD
  • ICAD-L
  • ICAD-S
Epitope
C-Term
37
29
18
16
13
13
11
9
5
4
4
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
165
78
32
1
1
Host
Rabbit
194
33
2
Clonality
Polyclonal
Conjugate
This DFFA antibody is un-conjugated
3
3
3
2
2
2
1
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Immunofluorescence (IF), Western Blotting (WB)
187
77
48
32
30
20
18
11
5
4
4
3
2
1
1
1
Supplier
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Catalog No. ABIN499742
$ 401.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.32106254 ABIN2452364 IF WB Mouse IgG1 AA 1-331 Log in to see 1B10
0.32106254 ABIN2452370 IF WB Mouse IgG1 Log in to see 5C4
0.32106254 ABIN2706027 IC IF IP IHC WB Rabbit Center Log in to see Polyclonal
0.32106254 ABIN514954 IF IP WB Rabbit AA 1-331, full length Log in to see Polyclonal
0.32106254 ABIN514953 IF WB Mouse AA 1-331, full length Log in to see Polyclonal
0.32106254 ABIN514955 IF WB Rabbit AA 1-331, full length Log in to see Polyclonal
0.0010843406 ABIN318989 IHC (fro) IF WB Rabbit IgG C-Term Log in to see Polyclonal 1
0.0010843406 ABIN968331 IF WB Mouse IgG1 AA 105-305 Log in to see 19-DFF45 5
0.0010843406 ABIN2452365 IF WB Mouse IgG1 Log in to see 3F12
0.0010843406 ABIN1498772 IF WB Mouse IgG1 Log in to see 3F12
0.0010843406 ABIN303253 IF IHC (p) IP WB Rabbit AA 313-331 Log in to see Polyclonal
0.0010843406 ABIN1498773 IF WB Mouse IgG1 Log in to see 5C4
0.0010843406 ABIN2892223 IF IHC WB Rabbit Gln177 Log in to see Polyclonal
0.0010843406 ABIN3185120 ELISA IF IHC WB Rabbit IgG Internal Region Log in to see Polyclonal
0.0010843406 ABIN2736635 IF IHC WB Rabbit IgG Log in to see Polyclonal
0.0010843406 ABIN1978399 IF IP IHC (p) WB Rabbit IgG AA 271-331 Log in to see Polyclonal
0.0010843406 ABIN1978400 IF IHC (p) Rabbit IgG AA 271-331 Log in to see Polyclonal
0.0010843406 ABIN2878569 IF Rabbit IgG Log in to see Polyclonal
0.0010843406 ABIN4305083 ICC IF WB Mouse IgG1 Transcript Variant 1 Log in to see 5C4
0.0010843406 ABIN2139072 IF WB Mouse IgG1 Log in to see 5C4

Top referenced anti-DFFA antibody for Immunofluorescence

Similar anti-DFFA Antibodies

Application / Reactivity Human
Dot Blot (DB) 4 Antibodies
ELISA 64 Antibodies
ELISA (Capture) 1 Antibodies
Enzyme Immunoassay (EIA) 4 Antibodies
Immunochromatography (IC) 4 Antibodies
Immunocytochemistry (ICC) 16 Antibodies
Immunofluorescence (IF) 29 Antibodies
Immunohistochemistry (IHC) 37 Antibodies
Immunohistochemistry (Acetone-fixed) (IHC (af)) 1 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 3 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 23 Antibodies
Immunoprecipitation (IP) 20 Antibodies
Proximity Ligation Assay (PLA) 2 Antibodies
Western Blotting (WB) 139 Antibodies

General

Antigen DNA Fragmentation Factor, 45kDa, alpha Polypeptide (DFFA) Antibodies
Epitope C-Term
(37), (29), (18), (16), (13), (13), (11), (9), (5), (4), (4), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(165), (78), (32), (1), (1)
Host Rabbit
(194), (33), (2)
Clonality
Polyclonal
Conjugate This DFFA antibody is un-conjugated
(3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Immunofluorescence (IF), Western Blotting (WB)
(187), (77), (48), (32), (30), (20), (18), (11), (5), (4), (4), (3), (2), (1), (1), (1)
Pubmed 5 references available
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Product Details anti-DFFA Antibody

Target Details DFFA Application Details Handling References for anti-DFFA Antibody (ABIN499742) Images
Specificity DFF45 antibody was raised against a peptide corresponding to amino acids 313 to 331 of human DFF45.
Purification DEAE
Immunogen Human DFF45 (C-Terminus) Peptide
Isotype IgG

Target Details DFFA

Product Details anti-DFFA Antibody Application Details Handling References for anti-DFFA Antibody (ABIN499742) Images back to top
Antigen
Alternative Name DFFA / ICAD (DFFA Antibody Abstract)
Background Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. These death signals finally cause the degradation of chromosomal DNA by activated DNase. A human 45 kDa DNA fragmentation factor (DFF45) was identified recently which was cleaved by caspase-3 during apoptosis. Mouse homologue of human DFF45 was identified as a DNase inhibitor designated ICAD (2,3). Upon cleavage of DFF45/ICAD, a caspase activated deoxyribonuclease (DFF40/CAD) is released and activated and eventually causes the degradation of DNA in the nuclei. Therefore, the cleavage of DFF45/ICAD, which causes DFF40/CAD activation and DNA degradation, is the hallmark of apoptotic cell death.Synonyms: DFF-45, DFF1, DFF45, DNA fragmentation factor 45 kDa subunit, DNA fragmentation factor subunit alpha, Inhibitor of CAD
Gene ID 1676
NCBI Accession NP_004392
UniProt O00273
Research Area Apoptosis/Necrosis
Pathways Apoptosis, Caspase Cascade in Apoptosis

Application Details

Product Details anti-DFFA Antibody Target Details DFFA Handling References for anti-DFFA Antibody (ABIN499742) Images back to top
Application Notes ELISA. Western Blot: DFF45 antibody can be used for detection of DFF45 and one of the cleavedfragments of DFF45 at 1/1000 to 1/2000 dilution. A 45 kDa band can be detected innon-apoptotic cells. Immunocytochemistry. Immunoprecipitation: DFF45 antibody can be used for detection of DFF45 and one of thecleaved fragments of DFF45 at 1/1000 to 1/2000 dilution.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-DFFA Antibody Target Details DFFA Application Details References for anti-DFFA Antibody (ABIN499742) Images back to top
Buffer PBS containing 0.02 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store the antibody undiluted at 2-8 °C.

References for anti-DFFA Antibody (ABIN499742)

Product Details anti-DFFA Antibody Target Details DFFA Application Details Handling Images back to top
Background publications

Nagai, Dohmae, Nishimura, Kato, Hirata, Takahashi: "Operando XAFS study of catalytic NO reduction over Cu/CeO2: the effect of copper-ceria interaction under periodic operation." in: Physical chemistry chemical physics : PCCP, Vol. 15, Issue 22, pp. 8461-5, 2013

Enari, Sakahira, Yokoyama, Okawa, Iwamatsu, Nagata: "A caspase-activated DNase that degrades DNA during apoptosis, and its inhibitor ICAD." in: Nature, Vol. 391, Issue 6662, pp. 43-50, 1998

Sakahira, Enari, Nagata: "Cleavage of CAD inhibitor in CAD activation and DNA degradation during apoptosis." in: Nature, Vol. 391, Issue 6662, pp. 96-9, 1998

Liu, Li, Widlak, Zou, Luo, Garrard, Wang: "The 40-kDa subunit of DNA fragmentation factor induces DNA fragmentation and chromatin condensation during apoptosis." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 95, Issue 15, pp. 8461-6, 1998

Liu, Zou, Slaughter, Wang: "DFF, a heterodimeric protein that functions downstream of caspase-3 to trigger DNA fragmentation during apoptosis." in: Cell, Vol. 89, Issue 2, pp. 175-84, 1997

Images

Product Details anti-DFFA Antibody Target Details DFFA Application Details Handling References for anti-DFFA Antibody (ABIN499742) back to top
Supplier Images
Immunofluorescence (IF) image for anti-DFFA antibody (DNA Fragmentation Factor, 45kDa, alpha Polypeptide) (C-Term) (ABIN499742) Immunocytochemistry of DFF45 in HeLa cells with AP30289PU-N DFF45 antibody at 5 μg/ml.
Western Blotting (WB) image for anti-DFFA antibody (DNA Fragmentation Factor, 45kDa, alpha Polypeptide) (C-Term) (ABIN499742) Western blot analysis of DFF45 in HeLa (H), Jurkat (J), A431 (A), and K562 (K) whole ...