We are preparing the requested document. Please wait, this may take a while...!
|Application / Reactivity||Human|
|Dot Blot (DB)||4 Antibodies|
|ELISA (Capture)||1 Antibodies|
|Enzyme Immunoassay (EIA)||4 Antibodies|
|Immunochromatography (IC)||4 Antibodies|
|Immunocytochemistry (ICC)||11 Antibodies|
|Immunofluorescence (IF)||24 Antibodies|
|Immunohistochemistry (IHC)||31 Antibodies|
|Immunohistochemistry (Acetone-fixed) (IHC (af))||1 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||3 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||17 Antibodies|
|Immunoprecipitation (IP)||20 Antibodies|
|Proximity Ligation Assay (PLA)||2 Antibodies|
|Western Blotting (WB)||133 Antibodies|
|Antigen||DNA Fragmentation Factor, 45kDa, alpha Polypeptide (DFFA) Antibodies|
|Conjugate||This DFFA antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Immunofluorescence (IF), Western Blotting (WB)
|5 references available|
|Supplier||Log in to see|
Product Details anti-DFFA AntibodyTarget Details DFFA Application Details Handling References for anti-DFFA Antibody (ABIN499742) Images
|Specificity||DFF45 antibody was raised against a peptide corresponding to amino acids 313 to 331 of human DFF45.|
|Immunogen||Human DFF45 (C-Terminus) Peptide|
Target Details DFFAProduct Details anti-DFFA Antibody Application Details Handling References for anti-DFFA Antibody (ABIN499742) Images back to top
|Alternative Name||DFFA / ICAD (DFFA Antibody Abstract)|
|Background||Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. These death signals finally cause the degradation of chromosomal DNA by activated DNase. A human 45 kDa DNA fragmentation factor (DFF45) was identified recently which was cleaved by caspase-3 during apoptosis. Mouse homologue of human DFF45 was identified as a DNase inhibitor designated ICAD (2,3). Upon cleavage of DFF45/ICAD, a caspase activated deoxyribonuclease (DFF40/CAD) is released and activated and eventually causes the degradation of DNA in the nuclei. Therefore, the cleavage of DFF45/ICAD, which causes DFF40/CAD activation and DNA degradation, is the hallmark of apoptotic cell death.Synonyms: DFF-45, DFF1, DFF45, DNA fragmentation factor 45 kDa subunit, DNA fragmentation factor subunit alpha, Inhibitor of CAD|
|Pathways||Apoptosis, Caspase Cascade in Apoptosis|
Application DetailsProduct Details anti-DFFA Antibody Target Details DFFA Handling References for anti-DFFA Antibody (ABIN499742) Images back to top
ELISA. Western Blot: DFF45 antibody can be used for detection of DFF45 and one of the cleavedfragments of DFF45 at 1/1000 to 1/2000 dilution. A 45 kDa band can be detected innon-apoptotic cells. Immunocytochemistry. Immunoprecipitation: DFF45 antibody can be used for detection of DFF45 and one of thecleaved fragments of DFF45 at 1/1000 to 1/2000 dilution.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-DFFA Antibody Target Details DFFA Application Details References for anti-DFFA Antibody (ABIN499742) Images back to top
|Buffer||PBS containing 0.02 % sodium azide.|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Storage Comment||Store the antibody undiluted at 2-8 °C.|
References for anti-DFFA Antibody (ABIN499742)Product Details anti-DFFA Antibody Target Details DFFA Application Details Handling Images back to top
Nagai, Dohmae, Nishimura et al.: "Operando XAFS study of catalytic NO reduction over Cu/CeO2: the effect of copper-ceria interaction under periodic operation." in: Physical chemistry chemical physics : PCCP, Vol. 15, Issue 22, pp. 8461-5, 2013 (PubMed).
Liu, Li, Widlak et al.: "The 40-kDa subunit of DNA fragmentation factor induces DNA fragmentation and chromatin condensation during apoptosis." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 95, Issue 15, pp. 8461-6, 1998 (PubMed).
Sakahira, Enari, Nagata: "Cleavage of CAD inhibitor in CAD activation and DNA degradation during apoptosis." in: Nature, Vol. 391, Issue 6662, pp. 96-9, 1998 (PubMed).
Enari, Sakahira, Yokoyama et al.: "A caspase-activated DNase that degrades DNA during apoptosis, and its inhibitor ICAD." in: Nature, Vol. 391, Issue 6662, pp. 43-50, 1998 (PubMed).
Liu, Zou, Slaughter et al.: "DFF, a heterodimeric protein that functions downstream of caspase-3 to trigger DNA fragmentation during apoptosis." in: Cell, Vol. 89, Issue 2, pp. 175-84, 1997 (PubMed).
ImagesProduct Details anti-DFFA Antibody Target Details DFFA Application Details Handling References for anti-DFFA Antibody (ABIN499742) back to top