anti-Mouse (Murine) DFFA antibody for Western Blotting

Recommended DFFA Antibody (supplied by: Log in to see )

Antigen
DNA Fragmentation Factor, 45kDa, alpha Polypeptide (DFFA) Antibodies
  • DFFA
  • DFF-45
  • DFF1
  • ICAD
  • A330085O09Rik
  • DFF35
  • Dff45
  • ICAD-L
  • ICAD-S
  • DNA fragmentation factor, 45kDa, alpha polypeptide
  • DNA fragmentation factor, alpha subunit
  • DFFA
  • dffa
  • Dffa
Epitope
N-Term
29
25
19
16
15
15
12
8
6
5
4
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
Reactivity
Cow (Bovine), Dog (Canine), Guinea Pig, Horse (Equine), Human, Mouse (Murine), Rat (Rattus)
167
84
40
2
1
Host
Rabbit
193
35
2
Clonality
Polyclonal
Conjugate
This DFFA antibody is un-conjugated
3
3
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Application
Western Blotting (WB)
192
71
48
37
34
28
19
13
6
4
4
3
2
2
1
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Catalog No. ABIN2779329
$ 229.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
3.2182703 ABIN1534436 IHC ELISA WB Rabbit IgG AA 151-200 Log in to see Polyclonal 1
3.2182703 ABIN3185120 ELISA IF IHC WB Rabbit IgG Internal Region Log in to see Polyclonal
3.2182703 ABIN1872254 IHC IP WB Rabbit IgG cleaved Log in to see Polyclonal
3.2182703 ABIN4305091 WB Rabbit IgG Center Log in to see Polyclonal
3.2182703 ABIN967295 WB Rabbit IgG AA 312-331 Log in to see Polyclonal 4
3.2182703 ABIN2474109 WB Rabbit IgG AA 312-331, C-Term Log in to see Polyclonal 3
3.2182703 ABIN2474110 IHC (p) WB Rabbit IgG AA 2-21, N-Term Log in to see Polyclonal 3
3.2182703 ABIN2855675 WB Rabbit IgG Center Log in to see Polyclonal
3.2182703 ABIN4305082 ICC IF IHC IHC (p) WB Rabbit Log in to see Polyclonal
3.2182703 ABIN4305084 ELISA IF IHC (p) WB Rabbit C-Term Log in to see Polyclonal
3.2182703 ABIN4305086 ELISA ICC IF IHC IHC (p) WB Rabbit Log in to see Polyclonal
3.2182703 ABIN2736635 IF IHC WB Rabbit IgG Log in to see Polyclonal
3.2182703 ABIN3024711 WB Rabbit IgG Log in to see Polyclonal
3.2182703 ABIN187736 WB Rabbit AA 312-331 Log in to see Polyclonal
3.2182703 ABIN1031406 IHC ELISA WB Rabbit N-Term Log in to see Polyclonal
3.2182703 ABIN1030428 ELISA WB Rabbit C-Term Log in to see Polyclonal
3.2182703 ABIN187735 WB Rabbit AA 2-21 Log in to see Polyclonal
1 ABIN222902 WB Rabbit IgG N-Term Log in to see Polyclonal 1
1 ABIN121553 IHC (p) WB Rabbit IgG AA 2-21, N-Term Log in to see Polyclonal 2
1 ABIN203383 WB Rabbit IgG N-Term Log in to see Polyclonal

General

Antigen DNA Fragmentation Factor, 45kDa, alpha Polypeptide (DFFA) Antibodies
Epitope N-Term
(29), (25), (19), (16), (15), (15), (12), (8), (6), (5), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Reactivity Cow (Bovine), Dog (Canine), Guinea Pig, Horse (Equine), Human, Mouse (Murine), Rat (Rattus)
(167), (84), (40), (2), (1)
Host Rabbit
(193), (35), (2)
Clonality
Polyclonal
Conjugate This DFFA antibody is un-conjugated
(3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Western Blotting (WB)
(192), (71), (48), (37), (34), (28), (19), (13), (6), (4), (4), (3), (2), (2), (1)
Pubmed 2 references available
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Product Details anti-DFFA Antibody

Target Details DFFA Application Details Handling References for anti-DFFA antibody (ABIN2779329) Images
Sequence MEVTGDAGVP ESGEIRTLKP CLLRRNYSRE QHGVAASCLE DLRSKACDIL
Predicted Reactivity Cow: 92%, Dog: 83%, Guinea Pig: 92%, Horse: 92%, Human: 100%, Mouse: 83%, Rat: 92%
Characteristics This is a rabbit polyclonal antibody against DFFA. It was validated on Western Blot using a cell lysate as a positive control.
Purification Protein A purified
Immunogen The immunogen is a synthetic peptide directed towards the N terminal region of human DFFA

Target Details DFFA

Product Details anti-DFFA Antibody Application Details Handling References for anti-DFFA antibody (ABIN2779329) Images back to top
Antigen
Alternative Name DFFA (DFFA Antibody Abstract)
Background Apoptosis is a cell death process that removes toxic and/or useless cells during mammalian development. The apoptotic process is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. DNA fragmentation factor (DFF) is a heterodimeric protein of 40-kD (DFFB) and 45-kD (DFFA) subunits. DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF becomes activated when DFFA is cleaved by caspase-3. The cleaved fragments of DFFA dissociate from DFFB, the active component of DFF. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis.Apoptosis is a cell death process that removes toxic and/or useless cells during mammalian development. The apoptotic process is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. DNA fragmentation factor (DFF) is a heterodimeric protein of 40-kD (DFFB) and 45-kD (DFFA) subunits. DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF becomes activated when DFFA is cleaved by caspase-3. The cleaved fragments of DFFA dissociate from DFFB, the active component of DFF. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.
Alias Symbols: DFF-45, DFF1, Ion ChannelAD, ICAD
Protein Interaction Partner: DFFB, UBC, TUBB, ATG3, ISOC1, BZW2, MAT2B, FERMT2, TUBA1A, CNBP, RPS6KA3, PPP5C, PFAS, MAT2A, EIF6, GAPDH, EIF5, CTPS1, CKB, GZMB, CASP7, CASP3, GZMM, TOM1L2, SEPT5, YWHAZ, YWHAG, PRKAA2, STRN4, NECAB2, CIDEB, EWSR1, NAP1L5, TMBIM4, USP15, HERC1, MCC, BAK
Protein Size: 331
Molecular Weight 37 kDa
Gene ID 1676
NCBI Accession NM_004401, NP_004392
UniProt O00273
Pathways Apoptosis, Caspase Cascade in Apoptosis

Application Details

Product Details anti-DFFA Antibody Target Details DFFA Handling References for anti-DFFA antibody (ABIN2779329) Images back to top
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 331 AA

Restrictions For Research Use only

Handling

Product Details anti-DFFA Antibody Target Details DFFA Application Details References for anti-DFFA antibody (ABIN2779329) Images back to top
Format Liquid
Concentration Lot specific
Buffer Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-DFFA antibody (ABIN2779329)

Product Details anti-DFFA Antibody Target Details DFFA Application Details Handling Images back to top
Background publications

Yan, Fu, Li, Blechl, Tranquilli, Bonafede, Sanchez, Valarik, Yasuda, Dubcovsky: "The wheat and barley vernalization gene VRN3 is an orthologue of FT." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 103, Issue 51, pp. 19581-6, 2006

Yan, Wang, Peng, Hu, Wang, Zhang, Chen, Bedford, Dewhirst, Li: "A unique role of the DNA fragmentation factor in maintaining genomic stability." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 103, Issue 5, pp. 1504-9, 2006

Images

Product Details anti-DFFA Antibody Target Details DFFA Application Details Handling References for anti-DFFA antibody (ABIN2779329) back to top
Supplier Images
Western Blotting (WB) image for anti-DNA Fragmentation Factor, 45kDa, alpha Polypeptide (DFFA) (N-Term) antibody (ABIN2779329) WB Suggested Anti-DFFA Antibody Titration: 5.0ug/ml ELISA Titer: 1:312500 Positive Co...