anti-Rat (Rattus) DFFA antibody for Immunohistochemistry

Recommended DFFA Antibody (supplied by: Log in to see )

Antigen
DNA Fragmentation Factor, 45kDa, alpha Polypeptide (DFFA) Antibodies
  • DFFA
  • DFF-45
  • DFF1
  • ICAD
  • A330085O09Rik
  • DFF35
  • Dff45
  • ICAD-L
  • ICAD-S
  • DNA fragmentation factor, 45kDa, alpha polypeptide
  • DNA fragmentation factor, alpha subunit
  • DFFA
  • dffa
  • Dffa
Epitope
AA 151-200
29
26
19
16
15
15
12
8
6
5
4
3
3
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
167
84
40
2
2
1
1
1
Host
Rabbit
193
35
2
Clonality
Polyclonal
Conjugate
This DFFA antibody is un-conjugated
3
3
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
192
70
47
37
34
28
19
13
6
4
4
3
2
2
1
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Catalog No. ABIN1534436
$ 302.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
8.870993 ABIN2736635 IF IHC WB Rabbit IgG Log in to see Polyclonal
8.870993 ABIN2428255 IHC ELISA Rabbit IgG Log in to see Polyclonal
8.870993 ABIN5543554 IHC ELISA Rabbit IgG Log in to see Polyclonal
1 ABIN1872254 IHC IP WB Rabbit IgG cleaved Log in to see Polyclonal
1 ABIN3185120 ELISA IF IHC WB Rabbit IgG Internal Region Log in to see Polyclonal
1 ABIN4305082 ICC IF IHC IHC (p) WB Rabbit Log in to see Polyclonal
1 ABIN1586053 IHC ELISA WB Rabbit N-Term Log in to see Polyclonal
1 ABIN1085306 IHC ELISA WB Rabbit IgG Log in to see Polyclonal
1 ABIN1085305 IHC ELISA WB Rabbit IgG Log in to see Polyclonal

General

Antigen DNA Fragmentation Factor, 45kDa, alpha Polypeptide (DFFA) Antibodies
Epitope AA 151-200
(29), (26), (19), (16), (15), (15), (12), (8), (6), (5), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(167), (84), (40), (2), (2), (1), (1), (1)
Host Rabbit
(193), (35), (2)
Clonality
Polyclonal
Conjugate This DFFA antibody is un-conjugated
(3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
(192), (70), (47), (37), (34), (28), (19), (13), (6), (4), (4), (3), (2), (2), (1)
Pubmed 1 reference available
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Product Details anti-DFFA Antibody

Target Details DFFA Application Details Handling References for anti-DFFA antibody (ABIN1534436) Images
Specificity

DFFA Antibody detects endogenous levels of total DFFA protein.

Purification The antibody was purified from rabbit antiserum by affinity-chromatography using immunogen.
Purity > 95 %
Immunogen The antiserum was produced against synthesized peptide derived from human DFFA.
Isotype IgG

Target Details DFFA

Product Details anti-DFFA Antibody Application Details Handling References for anti-DFFA antibody (ABIN1534436) Images back to top
Antigen
Alternative Name DFFA (DFFA Antibody Abstract)
Background

Synonyms: DFF-45, DFF1, DFF45, DNAation factor 45 kDa subunit, H13, ICAD, inhibitor of CAD

NCBI Gene Symbol: DFFA

Molecular Weight 36 kDa
Gene ID 1676
OMIM 601882
UniProt O00273
Pathways Apoptosis, Caspase Cascade in Apoptosis

Application Details

Product Details anti-DFFA Antibody Target Details DFFA Handling References for anti-DFFA antibody (ABIN1534436) Images back to top
Application Notes WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:20000
Comment

Unigene-Number: Hs.484782 (NCBI Gene Symbol: DFFA)

Restrictions For Research Use only

Handling

Product Details anti-DFFA Antibody Target Details DFFA Application Details References for anti-DFFA antibody (ABIN1534436) Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Stable at -20°C for at least 1 year.
Expiry Date 12 months

References for anti-DFFA antibody (ABIN1534436)

Product Details anti-DFFA Antibody Target Details DFFA Application Details Handling Images back to top
Background publications

Liu, Zou, Slaughter, Wang: "DFF, a heterodimeric protein that functions downstream of caspase-3 to trigger DNA fragmentation during apoptosis." in: Cell, Vol. 89, Issue 2, pp. 175-84, 1997

Images

Product Details anti-DFFA Antibody Target Details DFFA Application Details Handling References for anti-DFFA antibody (ABIN1534436) back to top
Supplier Images
Western Blotting (WB) image for anti-DNA Fragmentation Factor, 45kDa, alpha Polypeptide (DFFA) (AA 151-200) antibody (ABIN1534436) Western blot analysis of extracts from Jurkat/293/HeLa cells, using DFFA Antibody. Th...
Immunohistochemistry (IHC) image for anti-DNA Fragmentation Factor, 45kDa, alpha Polypeptide (DFFA) (AA 151-200) antibody (ABIN1534436) Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, usi...