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anti-Human DFFB antibody for Dot Blot

Recommended DFFB Antibody (supplied by: Log in to see )

Antigen
DNA Fragmentation Factor, 40kDa, beta Polypeptide (Caspase-Activated DNase) (DFFB) Antibodies
  • 40kDa
  • 5730477D02Rik
  • CAD
  • Cad
  • CPAN
  • DFF-40
  • DFF2
  • DFF40
  • Didff
  • LOC100223514
Reactivity
Human
101
69
49
1
Host
Mouse
126
9
Clonality
Monoclonal
Conjugate
This DFFB antibody is un-conjugated
5
5
4
2
2
2
2
2
2
2
2
2
2
2
2
Application
Dot Blot (DB)
107
38
25
22
21
18
17
5
4
3
2
1
Supplier
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Available images

Catalog No. ABIN931021
$ 285.71
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008906197 ABIN1831882 DB Mouse IgG1 Log in to see 2263C6a
0.0008906197 ABIN2598333 DB WB Mouse IgG1 Log in to see 2263C6a

Similar anti-DFFB Antibodies

Application / Reactivity Human
Dot Blot (DB) 3 Antibodies
ELISA 35 Antibodies
Enzyme Immunoassay (EIA) 3 Antibodies
Flow Cytometry (FACS) 17 Antibodies
Immunochromatography (IC) 3 Antibodies
Immunocytochemistry (ICC) 18 Antibodies
Immunofluorescence (IF) 4 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies
Immunohistochemistry (IHC) 19 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 14 Antibodies
Western Blotting (WB) 84 Antibodies

General

Antigen DNA Fragmentation Factor, 40kDa, beta Polypeptide (Caspase-Activated DNase) (DFFB) Antibodies
Reactivity Human
(101), (69), (49), (1)
Host Mouse
(126), (9)
Clonality (Clone)
Monoclonal   ( )
Conjugate This DFFB antibody is un-conjugated
(5), (5), (4), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2)
Application Dot Blot (DB)
(107), (38), (25), (22), (21), (18), (17), (5), (4), (3), (2), (1)
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Product Details anti-DFFB Antibody

Target Details DFFB Application Details Handling Images
Cross-Reactivity Human
Cross-Reactivity (Details) Other species not studied.
Purification Protein G affinity chromatography
Immunogen DFFB antibody was raised in mouse using recombinant Human Dna Fragmentation Factor, 40 Kda, Beta Polypeptide (Caspase-Activated Dnase) (Dffb)
Clone 2263C6a
Isotype IgG1

Target Details DFFB

Product Details anti-DFFB Antibody Application Details Handling Images back to top
Antigen
Alternative Name DFFB (DFFB Antibody Abstract)
Background DNA fragmentation factor subunit beta is a protein that in humans is encoded by the DFFB gene. Apoptosis is a cell death process that removes toxic and/or useless cells during mammalian development. The apoptotic process is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. DNA fragmentation factor (DFF) is a heterodimeric protein of 40kDa (DFFB) and 45kDa (DFFA) subunits. DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF becomes activated when DFFA is cleaved by caspase-3. The cleaved fragments of DFFA dissociate from DFFB, the active component of DFF. Synonyms: Monoclonal DFFB antibody, Anti-DFFB antibody, DNA fragmentation factor subunit beta antibody, CAD antibody, CPAN antibody, DFF2 antibody, DFF40 antibody, DFF-40 antibody.
Research Area Chromatin and Nuclear Signaling, Cell Cycle, Apoptosis/Necrosis, DNA/RNA
Pathways Apoptosis, Caspase Cascade in Apoptosis

Application Details

Product Details anti-DFFB Antibody Target Details DFFB Handling Images back to top
Application Notes Optimal conditions should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-DFFB Antibody Target Details DFFB Application Details Images back to top
Concentration 100 µg/mL
Buffer DFFB antibody in PBS (3.0 mM KCl, 1.5 mM KH2 PO4 , 140 mM NaCl, 8.0 mM Na2 HPO4 (pH 7.4)) containing 1 % bovine serum albumin (BSA) and 0.05 % sodium azide (NaN3 ).
Preservative Sodium azide
Precaution of Use This product contains Sodium Azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
Handling Advice Avoid repeated freeze/thaw cycles.
Dilute only prior to immediate use.
Storage 4 °C
Storage Comment Store at 2-8 °C for up to one year. We recommend long term storage at -20 °C.

Images

Product Details anti-DFFB Antibody Target Details DFFB Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-DFFB antibody (DNA Fragmentation Factor, 40kDa, beta Polypeptide (Caspase-Activated DNase)) (ABIN931021) anti-DNA Fragmentation Factor, 40kDa, beta Polypeptide (Caspase-Activated DNase) (DFFB) antibody