anti-Human DFFB antibody for Flow Cytometry

Recommended DFFB Antibody (supplied by: Log in to see )

Antigen
DNA Fragmentation Factor, 40kDa, beta Polypeptide (Caspase-Activated DNase) (DFFB) Antibodies
  • LOC100223514
  • CAD
  • CPAN
  • DFF-40
  • DFF2
  • DFF40
  • Cad
  • 40kDa
  • 5730477D02Rik
  • Didff
  • DNA fragmentation factor, 40kDa, beta polypeptide (caspase-activated DNase)
  • DNA fragmentation factor, beta subunit
  • DFFB
  • LOC100223514
  • Dffb
Epitope
AA 1-30, N-Term
18
17
15
8
5
5
4
4
4
4
3
3
3
3
2
1
1
1
1
1
1
1
Reactivity
Human
127
77
60
Host
Rabbit
129
29
Clonality
Polyclonal
Conjugate
This DFFB antibody is un-conjugated
6
5
5
3
3
3
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
128
38
26
26
21
20
17
10
5
3
1
1
1
Options
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Catalog No. ABIN653798
$ 324.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
14.05956 ABIN951896 FACS IHC (p) WB Rabbit Ig AA 1-30, N-Term Log in to see Polyclonal 4
1 ABIN2620018 ELISA FACS IHC WB Rabbit IgG AA 1-30 Log in to see Polyclonal
1 ABIN1965006 FACS IHC ELISA WB APC Rabbit IgG AA 1-30 Log in to see Polyclonal
1 ABIN1969103 FACS IHC ELISA WB Biotin Rabbit IgG AA 1-30 Log in to see Polyclonal
1 ABIN1962629 FACS IHC ELISA WB Alkaline Phosphatase (AP) Rabbit IgG AA 1-30 Log in to see Polyclonal
1 ABIN1971420 FACS IHC ELISA WB FITC Rabbit IgG AA 1-30 Log in to see Polyclonal
1 ABIN1973897 FACS IHC ELISA WB HRP Rabbit IgG AA 1-30 Log in to see Polyclonal
1 ABIN1976020 FACS IHC ELISA WB PE Rabbit IgG AA 1-30 Log in to see Polyclonal
1 ABIN2252778 FACS IHC ELISA WB PE Rabbit IgG N-Term, AA 1-30 Log in to see Polyclonal
1 ABIN2252774 FACS IHC ELISA WB APC Rabbit IgG N-Term, AA 1-30 Log in to see Polyclonal
1 ABIN2252772 FACS IHC ELISA WB Rabbit IgG N-Term, AA 1-30 Log in to see Polyclonal
1 ABIN2252776 FACS IHC ELISA WB FITC Rabbit IgG N-Term, AA 1-30 Log in to see Polyclonal
1 ABIN2252773 FACS IHC ELISA WB Alkaline Phosphatase (AP) Rabbit IgG N-Term, AA 1-30 Log in to see Polyclonal
1 ABIN2252775 FACS IHC ELISA WB Biotin Rabbit IgG N-Term, AA 1-30 Log in to see Polyclonal
1 ABIN1813546 FACS IHC (p) WB Rabbit AA 1-30 Log in to see Polyclonal
1 ABIN2620019 FACS IHC (p) WB Rabbit AA 203-238 Log in to see Polyclonal
1 ABIN2793806 FACS IHC WB Rabbit Ig Fraction N-Term Log in to see Polyclonal

General

Antigen DNA Fragmentation Factor, 40kDa, beta Polypeptide (Caspase-Activated DNase) (DFFB) Antibodies
Epitope AA 1-30, N-Term
(18), (17), (15), (8), (5), (5), (4), (4), (4), (4), (3), (3), (3), (3), (2), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(127), (77), (60)
Host Rabbit
(129), (29)
Clonality (Clone)
Polyclonal   ( )
Conjugate This DFFB antibody is un-conjugated
(6), (5), (5), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(128), (38), (26), (26), (21), (20), (17), (10), (5), (3), (1), (1), (1)
Pubmed 1 reference available
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Product Details anti-DFFB Antibody

Target Details DFFB Application Details Handling References for anti-DFFB antibody (ABIN653798) Images
Specificity This DFFB antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1~30 amino acids from the N-terminal region of human DFFB.
Purification This antibody is purified through a protein A column, followed by peptide affinity purification.
Immunogen This DFFB antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 AA from the N-terminal region of human DFFB.
Clone RB24419
Isotype Ig

Target Details DFFB

Product Details anti-DFFB Antibody Application Details Handling References for anti-DFFB antibody (ABIN653798) Images back to top
Antigen
Alternative Name DFFB (DFFB Antibody Abstract)
Background Apoptosis is a cell death process that removes toxic and/or useless cells during mammalian development. The apoptotic process is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. DNA fragmentation factor (DFF) is a heterodimeric protein of 40-kD (DFFB) and 45-kD (DFFA) subunits. DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF becomes activated when DFFA is cleaved by caspase-3. The cleaved fragments of DFFA dissociate from DFFB, the active component of DFF. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis.
Synonyms: Caspase-activated nuclease, CPAN, Caspase-activated deoxyribonuclease, Caspase-activated DNase, CAD, DNA fragmentation factor 40 kDa subunit, DFF-40, DNA fragmentation factor subunit beta,DFF40, DFF2, CAD, DFFB
Molecular Weight 39110 DA
Gene ID 1677
UniProt O76075
Research Area Apoptosis/Necrosis, Metabolism, Cell Structure
Pathways Apoptosis, Caspase Cascade in Apoptosis

Application Details

Product Details anti-DFFB Antibody Target Details DFFB Handling References for anti-DFFB antibody (ABIN653798) Images back to top
Application Notes WB = 1:1000, IHC (p) = 1:10-50, FACS = 1:10-50
Restrictions For Research Use only

Handling

Product Details anti-DFFB Antibody Target Details DFFB Application Details References for anti-DFFB antibody (ABIN653798) Images back to top
Format Liquid
Concentration 0.5 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months

References for anti-DFFB antibody (ABIN653798)

Product Details anti-DFFB Antibody Target Details DFFB Application Details Handling Images back to top
Background publications

Hristoskova, Holzgreve, Hahn, Rusterholz: "The chromatin of differentiating erythroblasts is cleaved into large size fragments independent of caspase activated DNase and apoptosis inducing factor." in: Journal of cellular physiology, Vol. 213, Issue 2, pp. 490-4, 2007

Images

Product Details anti-DFFB Antibody Target Details DFFB Application Details Handling References for anti-DFFB antibody (ABIN653798) back to top
Supplier Images
Western Blotting (WB) image for anti-DNA Fragmentation Factor, 40kDa, beta Polypeptide (Caspase-Activated DNase) (DFFB) (AA 1-30), (N-Term) antibody (ABIN653798) Western blot analysis of DFFB Antibody (N-term) (ABIN653798) in 293 cell line lysates...
Immunohistochemistry (IHC) image for anti-DNA Fragmentation Factor, 40kDa, beta Polypeptide (Caspase-Activated DNase) (DFFB) (AA 1-30), (N-Term) antibody (ABIN653798) DFFB Antibody (N-term) (ABIN653798) IHC analysis in formalin fixed and paraffin embed...
Flow Cytometry (FACS) image for anti-DNA Fragmentation Factor, 40kDa, beta Polypeptide (Caspase-Activated DNase) (DFFB) (AA 1-30), (N-Term) antibody (ABIN653798) DFFB Antibody (N-term) (ABIN653798) flow cytometric analysis of 293 cells (right hist...