anti-Human DFFB antibody for Immunohistochemistry (Paraffin-embedded Sections)

Recommended DFFB Antibody (supplied by: Log in to see )

Antigen
DNA Fragmentation Factor, 40kDa, beta Polypeptide (Caspase-Activated DNase) (DFFB) Antibodies
  • LOC100223514
  • CAD
  • CPAN
  • DFF-40
  • DFF2
  • DFF40
  • Cad
  • 40kDa
  • 5730477D02Rik
  • Didff
  • DNA fragmentation factor, 40kDa, beta polypeptide (caspase-activated DNase)
  • DNA fragmentation factor, beta subunit
  • DFFB
  • LOC100223514
  • Dffb
Epitope
AA 1-30, N-Term
18
17
15
8
5
5
4
4
4
4
3
3
3
3
2
1
1
1
1
1
1
1
Reactivity
Human
127
77
60
Host
Rabbit
129
29
Clonality
Polyclonal
Conjugate
This DFFB antibody is un-conjugated
6
5
5
3
3
3
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
128
38
26
26
21
20
17
10
5
3
1
1
1
Supplier
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Catalog No. ABIN951896
$ 390.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN653798 FACS IHC (p) WB Rabbit Ig AA 1-30, N-Term Log in to see Polyclonal 1
1 ABIN214484 ICC IHC (p) WB Rabbit Internal Region Log in to see Polyclonal
1 ABIN303254 IF IHC (p) WB Rabbit Center Log in to see Polyclonal
1 ABIN1733625 ICC IHC (p) WB Rabbit IgG Internal Region Log in to see Polyclonal
1 ABIN893946 IHC (p) WB HRP Rabbit IgG Log in to see Polyclonal
1 ABIN1101129 IHC (p) WB Rabbit IgG Log in to see Polyclonal
1 ABIN893938 IHC (p) WB Biotin Rabbit IgG Log in to see Polyclonal
1 ABIN872776 IF (p) IHC (p) WB Rabbit IgG Log in to see Polyclonal
1 ABIN1733626 IHC (p) WB Rabbit IgG Log in to see Polyclonal
1 ABIN1813546 FACS IHC (p) WB Rabbit AA 1-30 Log in to see Polyclonal
1 ABIN2620019 FACS IHC (p) WB Rabbit AA 203-238 Log in to see Polyclonal
1 ABIN2764718 IHC (p) WB Rabbit IgG Log in to see Polyclonal
1 ABIN2768562 IHC (p) IHC WB Rabbit Internal Region Log in to see Polyclonal

General

Antigen DNA Fragmentation Factor, 40kDa, beta Polypeptide (Caspase-Activated DNase) (DFFB) Antibodies
Epitope AA 1-30, N-Term
(18), (17), (15), (8), (5), (5), (4), (4), (4), (4), (3), (3), (3), (3), (2), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(127), (77), (60)
Host Rabbit
(129), (29)
Clonality
Polyclonal
Conjugate This DFFB antibody is un-conjugated
(6), (5), (5), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(128), (38), (26), (26), (21), (20), (17), (10), (5), (3), (1), (1), (1)
Pubmed 4 references available
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Product Details anti-DFFB Antibody

Target Details DFFB Application Details Handling References for anti-DFFB antibody (ABIN951896) Images
Specificity This antibody recognizes Human DFFB / CAD (N-term).
Purification Affinity Chromatography on Protein A
Immunogen KLH conjugated synthetic peptide between 1~30 amino acids from the N-terminal region of Human DFFB / CAD. Genename: DFFB
Isotype Ig

Target Details DFFB

Product Details anti-DFFB Antibody Application Details Handling References for anti-DFFB antibody (ABIN951896) Images back to top
Antigen
Alternative Name DFFB / CAD (DFFB Antibody Abstract)
Background Apoptosis is a cell death process that removes toxic and/or useless cells during mammalian development. The apoptotic process is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. DNA fragmentation factor (DFF) is a heterodimeric protein of 40-kD (DFFB) and 45-kD (DFFA) subunits. DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF becomes activated when DFFA is cleaved by caspase-3. The cleaved fragments of DFFA dissociate from DFFB, the active component of DFF. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosisSynonyms: CPAN, Caspase-activated DNase, Caspase-activated deoxyribonuclease, Caspase-activated nuclease, DFF-40, DFF2, DFF40, DNA fragmentation factor 40 kDa subunit, DNA fragmentation factor subunit beta
Molecular Weight 39110 Da
Gene ID 1677
NCBI Accession NP_004393
Pathways Apoptosis, Caspase Cascade in Apoptosis

Application Details

Product Details anti-DFFB Antibody Target Details DFFB Handling References for anti-DFFB antibody (ABIN951896) Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-DFFB Antibody Target Details DFFB Application Details References for anti-DFFB antibody (ABIN951896) Images back to top
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS, 0.09 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

References for anti-DFFB antibody (ABIN951896)

Product Details anti-DFFB Antibody Target Details DFFB Application Details Handling Images back to top
Background publications

Hanus, Kalinowska-Herok, Widlak: "The major apoptotic endonuclease DFF40/CAD is a deoxyribose-specific and double-strand-specific enzyme." in: Apoptosis : an international journal on programmed cell death, Vol. 13, Issue 3, pp. 377-82, 2008

Kalinowska-Herok, Wid��ak: "High mobility group proteins stimulate DNA cleavage by apoptotic endonuclease DFF40/CAD due to HMG-box interactions with DNA." in: Acta biochimica Polonica, Vol. 55, Issue 1, pp. 21-6, 2008

Neimanis, Albig, Doenecke, Kahle: "Sequence elements in both subunits of the DNA fragmentation factor are essential for its nuclear transport." in: The Journal of biological chemistry, Vol. 282, Issue 49, pp. 35821-30, 2007

Hristoskova, Holzgreve, Hahn, Rusterholz: "The chromatin of differentiating erythroblasts is cleaved into large size fragments independent of caspase activated DNase and apoptosis inducing factor." in: Journal of cellular physiology, Vol. 213, Issue 2, pp. 490-4, 2007

Images

Product Details anti-DFFB Antibody Target Details DFFB Application Details Handling References for anti-DFFB antibody (ABIN951896) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-DNA Fragmentation Factor, 40kDa, beta Polypeptide (Caspase-Activated DNase) (DFFB) (AA 1-30), (N-Term) antibody (ABIN951896) Flow cytometric analysis of 293 cells using DFFB / CAD Antibody (N-term) Cat.-No AP51...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-DNA Fragmentation Factor, 40kDa, beta Polypeptide (Caspase-Activated DNase) (DFFB) (AA 1-30), (N-Term) antibody (ABIN951896) Formalin fixed and paraffin embedded prostate carcinoma reacted with DFFB / CAD Antib...
Western Blotting (WB) image for anti-DNA Fragmentation Factor, 40kDa, beta Polypeptide (Caspase-Activated DNase) (DFFB) (AA 1-30), (N-Term) antibody (ABIN951896) Western blot analysis of DFFB / CAD Antibody (N-term) in 293 cell line lysates (35ug/...