anti-Mouse (Murine) DFFB antibody for Enzyme Immunoassay

Recommended DFFB Antibody (supplied by: Log in to see )

Antigen
DNA Fragmentation Factor, 40kDa, beta Polypeptide (Caspase-Activated DNase) (DFFB) Antibodies
  • LOC100223514
  • CAD
  • CPAN
  • DFF-40
  • DFF2
  • DFF40
  • Cad
  • 40kDa
  • 5730477D02Rik
  • Didff
  • DNA fragmentation factor, 40kDa, beta polypeptide (caspase-activated DNase)
  • DNA fragmentation factor, beta subunit
  • DFFB
  • LOC100223514
  • Dffb
Epitope
Intermediate Domain
19
18
15
8
5
5
4
4
4
4
3
3
3
3
2
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
127
76
59
Host
Rabbit
129
29
Clonality
Polyclonal
Conjugate
This DFFB antibody is un-conjugated
6
5
5
3
3
3
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Immunofluorescence (IF), Western Blotting (WB)
128
38
26
26
21
21
18
9
4
3
1
1
1
Supplier
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Catalog No. ABIN5554750
$ 375.38
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN5554638 EIA IHC (p) WB Rabbit IgG C-Term Log in to see Polyclonal
1 ABIN5554748 EIA IF WB Rabbit IgG Log in to see Polyclonal
1 ABIN5554637 EIA IHC (p) WB Rabbit IgG Log in to see Polyclonal

General

Antigen DNA Fragmentation Factor, 40kDa, beta Polypeptide (Caspase-Activated DNase) (DFFB) Antibodies
Epitope Intermediate Domain
(19), (18), (15), (8), (5), (5), (4), (4), (4), (4), (3), (3), (3), (3), (2), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(127), (76), (59)
Host Rabbit
(129), (29)
Clonality
Polyclonal
Conjugate This DFFB antibody is un-conjugated
(6), (5), (5), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Immunofluorescence (IF), Western Blotting (WB)
(128), (38), (26), (26), (21), (21), (18), (9), (4), (3), (1), (1), (1)
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Product Details anti-DFFB Antibody

Target Details DFFB Application Details Handling Images
Specificity DFF40 antibody was raised against a peptide corresponding to amino acids 203 to 218 of human DFF40.
Purification Affinity chromatography purified via peptide column
Immunogen Human DFF40 (Intermediate Domain) Peptide
Isotype IgG

Target Details DFFB

Product Details anti-DFFB Antibody Application Details Handling Images back to top
Antigen
Alternative Name DFFB / CAD (DFFB Antibody Abstract)
Background Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. These death signals finally cause the degradation of chromosomal DNA by activated DNase. A mouse DNase that causes DNA fragmentation was identified recently and designated CAD for caspase activated deoxyribonuclease (1,2). The human homologue of mouse CAD was more recently identified by three groups independently and termed CPAN, DFF40, and human CAD, respectively, (3-5). DFF45/ICAD is the inhibitory protein of DFF40/CAD (1,2,6) and forms complex with DFF40/CAD. Upon cleavage of DFF45/ICAD by activated caspase, DFF40/CAD is released and activated and eventually causes the degradation of DNA in the nuclei. Activation of DFF40/CAD, which causes DNA degradation, is the hallmark of apoptotic cell death.Synonyms: CPAN, Caspase-activated DNase, Caspase-activated deoxyribonuclease, Caspase-activated nuclease, DFF-40, DFF2, DFF40, DNA fragmentation factor 40 kDa subunit, DNA fragmentation factor subunit beta
Gene ID 1677
NCBI Accession NP_004393
UniProt O76075
Pathways Apoptosis, Caspase Cascade in Apoptosis

Application Details

Product Details anti-DFFB Antibody Target Details DFFB Handling Images back to top
Application Notes ELISA. Western Blot: 1/500. A 40 kDa band can be detected. Immunocytochemistry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-DFFB Antibody Target Details DFFB Application Details Images back to top
Buffer PBS containing 0.02 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store the antibody undiluted at 2-8 °C.