anti-Mouse (Murine) DFFB antibody for Western Blotting

Recommended DFFB Antibody (supplied by: Log in to see )

Antigen
DNA Fragmentation Factor, 40kDa, beta Polypeptide (Caspase-Activated DNase) (DFFB) Antibodies
  • LOC100223514
  • CAD
  • CPAN
  • DFF-40
  • DFF2
  • DFF40
  • Cad
  • 40kDa
  • 5730477D02Rik
  • Didff
  • DNA fragmentation factor, 40kDa, beta polypeptide (caspase-activated DNase)
  • DNA fragmentation factor, beta subunit
  • DFFB
  • LOC100223514
  • Dffb
Epitope
AA 203-218
19
18
15
8
5
5
4
4
4
3
3
3
3
3
2
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
127
76
59
Host
Rabbit
129
29
Clonality
Polyclonal
Conjugate
This DFFB antibody is un-conjugated
6
5
5
3
3
3
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Application
Western Blotting (WB)
128
38
26
26
21
21
18
10
5
3
1
1
1
Options
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Catalog No. ABIN967293
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
3.2182703 ABIN2707475 WB Rabbit N-Term Log in to see Polyclonal
3.2182703 ABIN4305065 ELISA ICC IF WB Rabbit Center Log in to see Polyclonal
3.2182703 ABIN4903473 WB Rabbit IgG Log in to see Polyclonal
3.2182703 ABIN4305068 ELISA ICC IF WB Rabbit Log in to see Polyclonal
3.2182703 ABIN1030918 ICC ELISA WB Rabbit Middle Region Log in to see Polyclonal
3.2182703 ABIN1030919 ICC ELISA WB Rabbit Middle Region Log in to see Polyclonal
3.2182703 ABIN187738 WB Rabbit AA 314-329 Log in to see Polyclonal
3.2182703 ABIN187737 WB Rabbit Log in to see Polyclonal
3.2182703 ABIN189872 WB Rabbit AA 314-329 Log in to see Polyclonal
3.2182703 ABIN189883 WB Rabbit AA 147-164 Log in to see Polyclonal
3.2182703 ABIN189899 WB Rabbit AA 203-218 Log in to see Polyclonal
3.2182703 ABIN189950 WB Rabbit AA 205-222 Log in to see Polyclonal
1 ABIN319000 IHC (p) WB Rabbit IgG AA 205-222 Log in to see Polyclonal 2
1 ABIN724085 IF (p) IHC (p) WB Rabbit IgG AA 190-240 Log in to see Polyclonal
1 ABIN214484 ICC IHC (p) WB Rabbit Internal Region Log in to see Polyclonal
1 ABIN5554750 EIA IF WB Rabbit IgG Intermediate Domain Log in to see Polyclonal
1 ABIN5554638 EIA IHC (p) WB Rabbit IgG C-Term Log in to see Polyclonal
1 ABIN5554748 EIA IF WB Rabbit IgG Log in to see Polyclonal
1 ABIN724094 IHC (p) WB HRP Rabbit IgG AA 190-240 Log in to see Polyclonal
1 ABIN5629854 ELISA ICC IF WB Rabbit IgG AA 130-180 Log in to see Polyclonal

General

Antigen DNA Fragmentation Factor, 40kDa, beta Polypeptide (Caspase-Activated DNase) (DFFB) Antibodies
Epitope AA 203-218
(19), (18), (15), (8), (5), (5), (4), (4), (4), (3), (3), (3), (3), (3), (2), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(127), (76), (59)
Host Rabbit
(129), (29)
Clonality
Polyclonal
Conjugate This DFFB antibody is un-conjugated
(6), (5), (5), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Western Blotting (WB)
(128), (38), (26), (26), (21), (21), (18), (10), (5), (3), (1), (1), (1)
Pubmed 4 references available
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Product Details anti-DFFB Antibody

Target Details DFFB Application Details Handling References for anti-DFFB antibody (ABIN967293) Images
Brand BD Pharmingen™
Cross-Reactivity Mouse (Murine), Rat (Rattus)
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
3. Please refer to us for technical protocols.
Purification The polyclonal antibody was purified from antiserum by affinity chromatography.
Immunogen Human DFF40 peptide aa. 203-218
Isotype Ig Fraction

Target Details DFFB

Product Details anti-DFFB Antibody Application Details Handling References for anti-DFFB antibody (ABIN967293) Images back to top
Antigen
Alternative Name DFF40/CAD (DFFB Antibody Abstract)
Background The DNA fragmentation factor (DFF) is a complex composed of two subunits, DFF40/CAD and DFF45. DFF40/CAD is the nuclease responsible for DNA fragmentation during apoptosis. DFF45 is a molecular chaperone that inhibits the activity of DFF40/CAD. During apoptosis, activated caspase-3 cleaves DFF45 (45 kDa) into 30 and 11 kDa fragments, resulting in the release and activation of DFF40. Activated DFF40 causes DNA fragmentation which has long been considered to be a hallmark of apoptotic cell death. DFF40 migrates at approximately 40 kDa in SDS/PAGE. The antibodies recognize human, mouse, and rat DFF40/CAD. A peptide corresponding to amino acids (a.a.) 203 to 218 of human DFF40 was used as the immunogen.
Molecular Weight 40 kDa
Research Area Apoptosis/Necrosis, DNA/RNA
Pathways Apoptosis, Caspase Cascade in Apoptosis

Application Details

Product Details anti-DFFB Antibody Target Details DFFB Handling References for anti-DFFB antibody (ABIN967293) Images back to top
Comment

Related Products: ABIN967390, ABIN968537

Restrictions For Research Use only

Handling

Product Details anti-DFFB Antibody Target Details DFFB Application Details References for anti-DFFB antibody (ABIN967293) Images back to top
Format Liquid
Concentration 0.5 mg/mL
Buffer Aqueous buffered solution containing ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store undiluted at 4°C. The polyclonal antibody was purified from antiserum by affinity chromatography.

References for anti-DFFB antibody (ABIN967293)

Product Details anti-DFFB Antibody Target Details DFFB Application Details Handling Images back to top
Product cited in:

Liu, Zou, Widlak, Garrard, Wang: "Activation of the apoptotic endonuclease DFF40 (caspase-activated DNase or nuclease). Oligomerization and direct interaction with histone H1." in: The Journal of biological chemistry, Vol. 274, Issue 20, pp. 13836-40, 1999

Enari, Sakahira, Yokoyama, Okawa, Iwamatsu, Nagata: "A caspase-activated DNase that degrades DNA during apoptosis, and its inhibitor ICAD." in: Nature, Vol. 391, Issue 6662, pp. 43-50, 1998

Liu, Li, Widlak, Zou, Luo, Garrard, Wang: "The 40-kDa subunit of DNA fragmentation factor induces DNA fragmentation and chromatin condensation during apoptosis." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 95, Issue 15, pp. 8461-6, 1998

Mukae, Enari, Sakahira, Fukuda, Inazawa, Toh, Nagata: "Molecular cloning and characterization of human caspase-activated DNase." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 95, Issue 16, pp. 9123-8, 1998

Images

Product Details anti-DFFB Antibody Target Details DFFB Application Details Handling References for anti-DFFB antibody (ABIN967293) back to top
Supplier Images
Western Blotting (WB) image for anti-DNA Fragmentation Factor, 40kDa, beta Polypeptide (Caspase-Activated DNase) (DFFB) (AA 203-218) antibody (ABIN967293) Western blot analysis of DFF40/CAD. A Jurkat cell lysate (Human T-cell leukemia, ATCC...