anti-Rat (Rattus) DFFB antibody for Immunohistochemistry (Paraffin-embedded Sections)

Recommended DFFB Antibody (supplied by: Log in to see )

Antigen
DNA Fragmentation Factor, 40kDa, beta Polypeptide (Caspase-Activated DNase) (DFFB) Antibodies
  • LOC100223514
  • CAD
  • CPAN
  • DFF-40
  • DFF2
  • DFF40
  • Cad
  • 40kDa
  • 5730477D02Rik
  • Didff
  • DNA fragmentation factor, 40kDa, beta polypeptide (caspase-activated DNase)
  • DNA fragmentation factor, beta subunit
  • DFFB
  • LOC100223514
  • Dffb
Epitope
Center
19
18
15
8
5
5
4
4
4
3
3
3
3
3
2
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
126
75
58
Host
Rabbit
128
29
Clonality
Polyclonal
Conjugate
This DFFB antibody is un-conjugated
6
5
5
3
3
3
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Application
Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
127
37
26
26
20
20
18
8
5
3
1
1
1
Supplier
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Available images

Catalog No. ABIN303254
$ 500.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN214484 ICC IHC (p) WB Rabbit Internal Region Log in to see Polyclonal
1 ABIN5554637 EIA IHC (p) WB Rabbit IgG Log in to see Polyclonal
1 ABIN724085 IF (p) IHC (p) WB Rabbit IgG AA 190-240 Log in to see Polyclonal
1 ABIN1733625 ICC IHC (p) WB Rabbit IgG Internal Region Log in to see Polyclonal
1 ABIN724094 IHC (p) WB HRP Rabbit IgG AA 190-240 Log in to see Polyclonal
1 ABIN724087 IHC (p) WB Biotin Rabbit IgG AA 190-240 Log in to see Polyclonal
1 ABIN893946 IHC (p) WB HRP Rabbit IgG Log in to see Polyclonal
1 ABIN893938 IHC (p) WB Biotin Rabbit IgG Log in to see Polyclonal
1 ABIN872776 IF (p) IHC (p) WB Rabbit IgG Log in to see Polyclonal

Similar anti-DFFB Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine) Human
ELISA 10 Antibodies 11 Antibodies 36 Antibodies
Enzyme Immunoassay (EIA) 3 Antibodies 4 Antibodies
Immunocytochemistry (ICC) 12 Antibodies 12 Antibodies
Immunofluorescence (IF) 6 Antibodies 6 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 26 Antibodies 26 Antibodies
Immunohistochemistry (IHC) 3 Antibodies 6 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 10 Antibodies 13 Antibodies
Western Blotting (WB) 34 Antibodies 51 Antibodies
Dot Blot (DB) 3 Antibodies

General

Antigen DNA Fragmentation Factor, 40kDa, beta Polypeptide (Caspase-Activated DNase) (DFFB) Antibodies
Epitope Center
(19), (18), (15), (8), (5), (5), (4), (4), (4), (3), (3), (3), (3), (3), (2), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(126), (75), (58)
Host Rabbit
(128), (29)
Clonality
Polyclonal
Conjugate This DFFB antibody is un-conjugated
(6), (5), (5), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(127), (37), (26), (26), (20), (20), (18), (8), (5), (3), (1), (1), (1)
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Product Details anti-DFFB Antibody

Target Details DFFB Application Details Handling Images
Purification Immunoaffinity Chromatography.
Immunogen Synthetic Peptide corresponding to amino acids near the center of murine CAD.

Target Details DFFB

Product Details anti-DFFB Antibody Application Details Handling Images back to top
Antigen
Alternative Name DFFB / CAD (DFFB Antibody Abstract)
Background Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. These death signals finally cause the degradation of chromosomal DNA by activated DNase. A mouse DNase that causes DNA fragmentation was identified recently and designated CAD (for caspase activated deoxyribonuclease). The human homologue of mouse CAD was more recently identified by two groups independently and termed CPAN and DFF40. Human DFF45 and its mouse homologue ICAD are the inhibitors of CPAN/DFF40 and CAD, respectively (1, 2, 5). Upon cleavage of DFF45/ICAD by activated caspase, DFF40/CAD is released and activated and eventually causes the degradation of DNA in the nuclei. Activation of CAD/DFF40, which causes DNA degradation, is the hallmark of apoptotic cell death.Synonyms: CPAN, Caspase-activated DNase, Caspase-activated deoxyribonuclease, Caspase-activated nuclease, DFF-40, DFF2, DFF40, DNA fragmentation factor 40 kDa subunit, DNA fragmentation factor subunit beta
Gene ID 1677
NCBI Accession NP_004393
UniProt O76075
Pathways Apoptosis, Caspase Cascade in Apoptosis

Application Details

Product Details anti-DFFB Antibody Target Details DFFB Handling Images back to top
Application Notes Immunohistochemistry on Paraffin Sections (5 μg/mL). Immunocytochemistry. Western Blot (1/500-1/1000).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-DFFB Antibody Target Details DFFB Application Details Images back to top
Buffer Phosphate Buffered Saline PBS containing 0.02 % Sodium Azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C to -70 °C for longer. Dilute only prior to immediate use.

Images

Product Details anti-DFFB Antibody Target Details DFFB Application Details Handling back to top
Supplier Images
 image for anti-DFFB antibody (DNA Fragmentation Factor, 40kDa, beta Polypeptide (Caspase-Activated DNase)) (Center) (ABIN303254) anti-DNA Fragmentation Factor, 40kDa, beta Polypeptide (Caspase-Activated DNase) (DFFB) (Center) antibody