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anti-Rat (Rattus) DFFB antibody for Immunohistochemistry (Paraffin-embedded Sections)

Recommended DFFB Antibody (supplied by: Log in to see )

Antigen
DNA Fragmentation Factor, 40kDa, beta Polypeptide (Caspase-Activated DNase) (DFFB) Antibodies
  • 40kDa
  • 5730477D02Rik
  • CAD
  • Cad
  • CPAN
  • DFF-40
  • DFF2
  • DFF40
  • Didff
  • LOC100223514
Reactivity
Mouse (Murine), Rat (Rattus)
126
71
51
1
Host
Rabbit
131
28
Clonality
Polyclonal
Conjugate
This DFFB antibody is un-conjugated
6
5
5
3
3
3
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
130
42
25
22
22
20
17
8
4
3
3
1
Supplier
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Catalog No. ABIN499515
$ 401.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.00089690165 ABIN724085 IF (p) IHC (p) WB Rabbit IgG AA 190-240 Log in to see Polyclonal
0.00089690165 ABIN303254 IF IHC (p) WB Rabbit Center Log in to see Polyclonal
0.00089690165 ABIN724087 IHC (p) WB Biotin Rabbit IgG AA 190-240 Log in to see Polyclonal
0.00089690165 ABIN724094 IHC (p) WB HRP Rabbit IgG AA 190-240 Log in to see Polyclonal
0.00089690165 ABIN1733625 ICC IHC (p) WB Rabbit IgG Internal Region Log in to see Polyclonal
0.00089690165 ABIN893938 IHC (p) WB Biotin Rabbit IgG Log in to see Polyclonal
0.00089690165 ABIN893946 IHC (p) WB HRP Rabbit IgG Log in to see Polyclonal
0.00089690165 ABIN872776 IF (p) IHC (p) WB Rabbit IgG Log in to see Polyclonal

Top referenced anti-DFFB antibody for Immunohistochemistry (Paraffin-embedded Sections)

Similar anti-DFFB Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine)
ELISA 11 Antibodies 13 Antibodies
Enzyme Immunoassay (EIA) 3 Antibodies 4 Antibodies
Immunocytochemistry (ICC) 12 Antibodies 12 Antibodies
Immunofluorescence (IF) 6 Antibodies 6 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 22 Antibodies 22 Antibodies
Immunohistochemistry (IHC) 1 Antibodies 7 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 9 Antibodies 12 Antibodies
Western Blotting (WB) 31 Antibodies 51 Antibodies

General

Antigen DNA Fragmentation Factor, 40kDa, beta Polypeptide (Caspase-Activated DNase) (DFFB) Antibodies
Reactivity Mouse (Murine), Rat (Rattus)
(126), (71), (51), (1)
Host Rabbit
(131), (28)
Clonality
Polyclonal
Conjugate This DFFB antibody is un-conjugated
(6), (5), (5), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(130), (42), (25), (22), (22), (20), (17), (8), (4), (3), (3), (1)
Pubmed 5 references available
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Product Details anti-DFFB Antibody

Target Details DFFB Application Details Handling References for anti-DFFB Antibody (ABIN499515) Images
Specificity CAD antibody was raised against a peptide corresponding to 17 amino acids near the center of murine CAD.
Purification Affinity chromatography purified via peptide column
Immunogen Human CAD (I17) Peptide
Isotype IgG

Target Details DFFB

Product Details anti-DFFB Antibody Application Details Handling References for anti-DFFB Antibody (ABIN499515) Images back to top
Antigen
Alternative Name DFFB / CAD (DFFB Antibody Abstract)
Background Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. These death signals finally cause the degradation of chromosomal DNA by activated DNase. A mouse DNase that causes DNA fragmentation was identified recently and designated CAD (for caspase activated deoxyribonuclease). The human homologue of mouse CAD was more recently identified by two groups independently and termed CPAN and DFF40. Human DFF45 and its mouse homologue ICAD are the inhibitors of CPAN/DFF40 and CAD, respectively. Upon cleavage of DFF45/ICAD by activated caspase, DFF40/CAD is released and activated and eventually causes the degradation of DNA in the nuclei. Activation of CAD/DFF40, which causes DNA degradation, is the hallmark of apoptotic cell death.Synonyms: CPAN, Caspase-activated DNase, Caspase-activated deoxyribonuclease, Caspase-activated nuclease, DFF-40, DFF2, DFF40, DNA fragmentation factor 40 kDa subunit, DNA fragmentation factor subunit beta
Gene ID 13368
UniProt O54788
Pathways Apoptosis, Caspase Cascade in Apoptosis

Application Details

Product Details anti-DFFB Antibody Target Details DFFB Handling References for anti-DFFB Antibody (ABIN499515) Images back to top
Application Notes ELISA. Western Blot: CAD antibody can be used for detection of CAD at 0.5 - 1 μg/mL. A 40 kDaband should be detected. Immunohistochemistry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-DFFB Antibody Target Details DFFB Application Details References for anti-DFFB Antibody (ABIN499515) Images back to top
Buffer PBS containing 0.02 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store the antibody undiluted at 2-8 °C.

References for anti-DFFB Antibody (ABIN499515)

Product Details anti-DFFB Antibody Target Details DFFB Application Details Handling Images back to top
Background publications

Nagai, Dohmae, Nishimura, Kato, Hirata, Takahashi: "Operando XAFS study of catalytic NO reduction over Cu/CeO2: the effect of copper-ceria interaction under periodic operation." in: Physical chemistry chemical physics : PCCP, Vol. 15, Issue 22, pp. 8461-5, 2013

Enari, Sakahira, Yokoyama, Okawa, Iwamatsu, Nagata: "A caspase-activated DNase that degrades DNA during apoptosis, and its inhibitor ICAD." in: Nature, Vol. 391, Issue 6662, pp. 43-50, 1998

Sakahira, Enari, Nagata: "Cleavage of CAD inhibitor in CAD activation and DNA degradation during apoptosis." in: Nature, Vol. 391, Issue 6662, pp. 96-9, 1998

Liu, Li, Widlak, Zou, Luo, Garrard, Wang: "The 40-kDa subunit of DNA fragmentation factor induces DNA fragmentation and chromatin condensation during apoptosis." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 95, Issue 15, pp. 8461-6, 1998

Halenbeck, MacDonald, Roulston, Chen, Conroy, Williams: "CPAN, a human nuclease regulated by the caspase-sensitive inhibitor DFF45." in: Current biology : CB, Vol. 8, Issue 9, pp. 537-40, 1998

Images

Product Details anti-DFFB Antibody Target Details DFFB Application Details Handling References for anti-DFFB Antibody (ABIN499515) back to top
Supplier Images
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-DFFB antibody (DNA Fragmentation Factor, 40kDa, beta Polypeptide (Caspase-Activated DNase)) (ABIN499515) Immunohistochemistry of CAD in mouse kidney tissue with CAD antibody at 2 μg/ml.
Western Blotting (WB) image for anti-DFFB antibody (DNA Fragmentation Factor, 40kDa, beta Polypeptide (Caspase-Activated DNase)) (ABIN499515) Western blot analysis of CAD in mouse kidney tissue lysate with AP30174PU-N CAD antib...