You are viewing an incomplete version of our website. Please click to reload the website as full version.

anti-Human DIABLO antibody for Immunocytochemistry

Recommended DIABLO Antibody (supplied by: Log in to see )

Antigen
Second Mitochondria-Derived Activator of Caspase (DIABLO) Antibodies
  • 0610041G12Rik
  • 1700006L01Rik
  • AU040403
  • DBO
  • DFNA64
  • diablo-s
  • SMAC
  • Smac
  • smac
  • smac3
  • XSmac
Reactivity
Human
169
62
42
4
4
3
2
1
1
1
Host
Rabbit
128
50
9
1
Clonality
Polyclonal
Conjugate
This DIABLO antibody is un-conjugated
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
176
78
67
44
30
16
11
9
6
4
2
2
2
1
1
1
1
1
1
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

It's quick and easy to submit your validation proposal. I want to validate this product

Learn more

Available images

Catalog No. ABIN252299
Contact our Customer Service for availability and price in your country.
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0009990651 ABIN153019 ICC IF IP WB Mouse IgG3, IgG1 Log in to see 78-1-118 9
0.0009990651 ABIN218658 ICC IF IHC (p) IP ELISA WB Mouse IgG1 Log in to see
0.0009990651 ABIN4354645 ELISA ICC IF IHC IHC (p) WB Rabbit Log in to see Polyclonal 1
0.0009990651 ABIN2505678 ICC FACS IP ELISA WB Rat IgG2a AA 55-237 Log in to see 10G7
0.0009990651 ABIN4354641 ICC IF IHC (p) WB Rabbit Log in to see Polyclonal
0.0009990651 ABIN2974020 ICC IF IHC WB Rabbit IgG Log in to see Polyclonal
0.0009990651 ABIN4354646 ICC IF IHC IHC (p) WB Rabbit IgG Log in to see Polyclonal
0.0009990651 ABIN199860 ICC FACS IP ELISA WB Rat IgG2a Log in to see

Top referenced anti-DIABLO antibody for Immunocytochemistry

Similar anti-DIABLO Antibodies

Application / Reactivity Human
Cellular Assay (CA) 1 Antibodies
Dot Blot (DB) 2 Antibodies
ELISA 70 Antibodies
Enzyme Immunoassay (EIA) 2 Antibodies
Flow Cytometry (FACS) 4 Antibodies
Immunoassay (IA) 1 Antibodies
Immunochromatography (IC) 2 Antibodies
Immunocytochemistry (ICC) 9 Antibodies
Immunofluorescence (IF) 16 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 1 Antibodies
Immunohistochemistry (IHC) 56 Antibodies
Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)) 1 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 4 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 39 Antibodies
Immunoprecipitation (IP) 24 Antibodies
Immunostaining (ISt) 1 Antibodies
Neutralization (Neut) 1 Antibodies
Western Blotting (WB) 159 Antibodies

General

Antigen Second Mitochondria-Derived Activator of Caspase (DIABLO) Antibodies
Reactivity Human
(169), (62), (42), (4), (4), (3), (2), (1), (1), (1)
Host Rabbit
(128), (50), (9), (1)
Clonality
Polyclonal
Conjugate This DIABLO antibody is un-conjugated
(2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(176), (78), (67), (44), (30), (16), (11), (9), (6), (4), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Pubmed 16 references available
Supplier Log in to see

Product Details anti-DIABLO Antibody

Target Details DIABLO Application Details Handling References for anti-DIABLO Antibody (ABIN252299) Images
Specificity Smac
Purification Protein G purified
Immunogen This rabbit polyclonal antibody was developed against two synthetic peptides corresponding to amino acids 199 to 212 and amino acids 226 to 239 of human Smac (Genbank accession no. AAF87716).
Isotype IgG

Target Details DIABLO

Product Details anti-DIABLO Antibody Application Details Handling References for anti-DIABLO Antibody (ABIN252299) Images back to top
Antigen
Alternative Name SMAC/Diablo (DIABLO Antibody Abstract)
Background Gene Symbol: DIABLO
Gene ID 56616
UniProt Q9NR28
Pathways Apoptosis, Caspase Cascade in Apoptosis

Application Details

Product Details anti-DIABLO Antibody Target Details DIABLO Handling References for anti-DIABLO Antibody (ABIN252299) Images back to top
Application Notes Western Blot 0.5-2 μg/mL, Immunocytochemistry/Immunofluorescence 1:10-1:500, Immunohistochemistry-Paraffin 1:10-1:500
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only

Handling

Product Details anti-DIABLO Antibody Target Details DIABLO Application Details References for anti-DIABLO Antibody (ABIN252299) Images back to top
Format Liquid
Concentration 0.5 mg/mL
Buffer PBS
Buffer contains: 0.02 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles
Storage 4 °C,-20 °C
Storage Comment Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

References for anti-DIABLO Antibody (ABIN252299)

Product Details anti-DIABLO Antibody Target Details DIABLO Application Details Handling Images back to top
Product cited in:

Qiu, Liu, Sebastini, Sun, Wang, Zhang, Yu: "An apoptosis-independent role of SMAC in tumor suppression." in: Oncogene, Vol. 32, Issue 19, pp. 2380-9, 2013 (Sample species: Mouse (Murine)). Further details: Western Blotting,Immunohistochemistry (Paraffin-embedded Sections)

Endo, Kohnoe, Watanabe, Tashiro, Sakata, Morita, Kakeji, Maehara: "Clinical significance of Smac/DIABLO expression in colorectal cancer." in: Oncology reports, Vol. 21, Issue 2, pp. 351-5, 2009 (Sample species: Human).

Conus, Perozzo, Reinheckel, Peters, Scapozza, Yousefi, Simon: "Caspase-8 is activated by cathepsin D initiating neutrophil apoptosis during the resolution of inflammation." in: The Journal of experimental medicine, Vol. 205, Issue 3, pp. 685-98, 2008

Liu, Biddle, Hanks, Brouha, Yan, Lee, Leachman, Grossman: "Activation of dual apoptotic pathways in human melanocytes and protection by survivin." in: The Journal of investigative dermatology, Vol. 126, Issue 10, pp. 2247-56, 2006 (Sample species: Human). Further details: Western Blotting

Ren, Akyurek, Schlette, Rassidakis, Medeiros: "Expression of Smac/DIABLO in B-cell non-Hodgkin and Hodgkin lymphomas." in: Human pathology, Vol. 37, Issue 11, pp. 1407-13, 2006 Further details: Western Blotting,Immunohistochemistry (Paraffin-embedded Sections)

Mizutani, Nakanishi, Yamamoto, Li, Matsubara, Mikami, Okihara, Kawauchi, Bonavida, Miki: "Downregulation of Smac/DIABLO expression in renal cell carcinoma and its prognostic significance." in: Journal of clinical oncology : official journal of the American Society of Clinical Oncology, Vol. 23, Issue 3, pp. 448-54, 2005 (Sample species: Human). Further details: Western Blotting

Lee, Lee, Su, Huang, Liu, Lin, Won: "Justicidin A decreases the level of cytosolic Ku70 leading to apoptosis in human colorectal cancer cells." in: Carcinogenesis, Vol. 26, Issue 10, pp. 1716-30, 2005 Further details: Western Blotting

Berthier, Lemaire-Ewing, Prunet, Montange, Vejux, Pais de Barros, Monier, Gambert, Lizard, Néel: "7-Ketocholesterol-induced apoptosis. Involvement of several pro-apoptotic but also anti-apoptotic calcium-dependent transduction pathways." in: The FEBS journal, Vol. 272, Issue 12, pp. 3093-104, 2005 Further details: Western Blotting

Qin, Ziffra, Stennett, Bodner, Bonish, Chaturvedi, Bennett, Pollock, Trent, Hendrix, Rizzo, Miele, Nickoloff: "Proteasome inhibitors trigger NOXA-mediated apoptosis in melanoma and myeloma cells." in: Cancer research, Vol. 65, Issue 14, pp. 6282-93, 2005

Wu, Parungo, Wu, Kang, Laham, Sellke, Simons, Li: "PR39 inhibits apoptosis in hypoxic endothelial cells: role of inhibitor apoptosis protein-2." in: Circulation, Vol. 109, Issue 13, pp. 1660-7, 2004 Further details: Western Blotting

Qin, Stennett, Bacon, Bodner, Hendrix, Seftor, Seftor, Margaryan, Pollock, Curtis, Trent, Bennett, Miele, Nickoloff: "p53-independent NOXA induction overcomes apoptotic resistance of malignant melanomas." in: Molecular cancer therapeutics, Vol. 3, Issue 8, pp. 895-902, 2004 Further details: Western Blotting

Hu, Han, Couvillon, Exton: "Critical role of endogenous Akt/IAPs and MEK1/ERK pathways in counteracting endoplasmic reticulum stress-induced cell death." in: The Journal of biological chemistry, Vol. 279, Issue 47, pp. 49420-9, 2004 Further details: Immunocytochemistry,Immunofluorescence

Liu, Brouha, Grossman: "Rapid induction of mitochondrial events and caspase-independent apoptosis in Survivin-targeted melanoma cells." in: Oncogene, Vol. 23, Issue 1, pp. 39-48, 2004

Yoo, Kim, Kim, Park, Park, Jeon, Jung, Lee, Lee: "Immunohistochemical analysis of Smac/DIABLO expression in human carcinomas and sarcomas." in: APMIS : acta pathologica, microbiologica, et immunologica Scandinavica, Vol. 111, Issue 3, pp. 382-8, 2003

Liu, Dai, Chen, Durrant, Freeman, Liu, Grossman, Lee: "Phospholipid scramblase 3 controls mitochondrial structure, function, and apoptotic response." in: Molecular cancer research : MCR, Vol. 1, Issue 12, pp. 892-902, 2003

Vucic, Deshayes, Ackerly, Pisabarro, Kadkhodayan, Fairbrother, Dixit: "SMAC negatively regulates the anti-apoptotic activity of melanoma inhibitor of apoptosis (ML-IAP)." in: The Journal of biological chemistry, Vol. 277, Issue 14, pp. 12275-9, 2002

Images

Product Details anti-DIABLO Antibody Target Details DIABLO Application Details Handling References for anti-DIABLO Antibody (ABIN252299) back to top
Supplier Images
Western Blotting (WB) image for anti-DIABLO antibody (Second Mitochondria-Derived Activator of Caspase) (ABIN252299) Western blot analysis of Smac/DIABLO in HeLa cell lysate.
Immunofluorescence (IF) image for anti-DIABLO antibody (Second Mitochondria-Derived Activator of Caspase) (ABIN252299) Immunocytochemistry/Immunofluorescence: SMAC/Diablo Antibody [ABIN2522991] - Analysis...