anti-Human DIABLO antibody for Immunohistochemistry (Paraffin-embedded Sections)

Recommended DIABLO Antibody (supplied by: Log in to see )

Antigen
Second Mitochondria-Derived Activator of Caspase (DIABLO) Antibodies
  • smac
  • smac3
  • diablo-s
  • 0610041G12Rik
  • 1700006L01Rik
  • AU040403
  • Smac
  • DFNA64
  • SMAC
  • DBO
  • XSmac
  • diablo, IAP-binding mitochondrial protein
  • diablo homolog (Drosophila)
  • diablo
  • Diablo
  • DIABLO
Epitope
AA 56-239
38
32
13
11
8
6
4
4
4
3
2
2
2
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
184
70
56
4
4
3
3
3
3
2
1
1
Host
Rabbit
135
53
7
1
Clonality
Polyclonal
Conjugate
This DIABLO antibody is un-conjugated
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), ELISA, Western Blotting (WB)
182
72
66
48
31
20
9
8
7
4
4
3
2
2
1
1
1
1
1
Options
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Catalog No. ABIN3044515
$ 240.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
13.400779 ABIN4354645 ELISA ICC IF IHC IHC (p) IP WB Rabbit C-Term Log in to see Polyclonal 1
13.400779 ABIN4354640 IHC IHC (p) IP WB Rabbit Log in to see Polyclonal 1
13.400779 ABIN213924 IHC (p) WB Rabbit AA 222-237 Log in to see Polyclonal
13.400779 ABIN462354 IHC (p) WB Mouse IgG2b Log in to see 79-1-83
13.400779 ABIN4354644 ELISA IHC IHC (p) IP WB Rabbit C-Term Log in to see Polyclonal
13.400779 ABIN4354638 IHC IHC (p) IP WB Rabbit Log in to see Polyclonal
13.400779 ABIN4354643 IHC (p) WB Rabbit Log in to see Polyclonal
13.400779 ABIN4354646 ICC IF IHC IHC (p) WB Rabbit IgG Log in to see Polyclonal
13.400779 ABIN252164 IHC IHC (fro) IHC (p) IP SimWes WB Rabbit Log in to see Polyclonal
1 ABIN674619 IF (p) IHC (p) WB Rabbit IgG AA 177-211 Log in to see Polyclonal 1
1 ABIN252299 ICC IF IHC (p) WB Rabbit Log in to see Polyclonal 16
1 ABIN2476525 IHC (p) WB Rabbit IgG AA 225-239, C-Term Log in to see Polyclonal 2
1 ABIN492563 IHC (p) WB Mouse IgG2b Log in to see 79-1-83
1 ABIN5555253 EIA IHC (p) WB Rabbit IgG AA 222-237, C-Term Log in to see Polyclonal
1 ABIN302807 IHC (p) WB Rabbit AA 222-237 Log in to see Polyclonal
1 ABIN121596 IHC (p) WB Rabbit IgG AA 222-237 Log in to see Polyclonal
1 ABIN121595 IHC (p) WB Rabbit IgG AA 225-239 Log in to see Polyclonal
1 ABIN957206 IHC (fro) IHC (p) IP WB Rabbit AA 56-73 Log in to see Polyclonal
1 ABIN2476526 IHC (p) WB Rabbit IgG C-Term Log in to see Polyclonal 2
1 ABIN959862 IHC (p) WB Rabbit IgG AA 199-212 Log in to see Polyclonal

Top referenced anti-DIABLO antibody for Immunohistochemistry (Paraffin-embedded Sections)

Similar anti-DIABLO Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine)
ELISA 17 Antibodies 23 Antibodies
Enzyme Immunoassay (EIA) 1 Antibodies 1 Antibodies
Immunochromatography (IC) 1 Antibodies 3 Antibodies
Immunocytochemistry (ICC) 3 Antibodies 5 Antibodies
Immunofluorescence (IF) 9 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 1 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 1 Antibodies
Immunohistochemistry (IHC) 33 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 3 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 20 Antibodies
Immunoprecipitation (IP) 11 Antibodies
Simple Western (SimWes) 1 Antibodies
Western Blotting (WB) 55 Antibodies
Flow Cytometry (FACS) 5 Antibodies

General

Antigen Second Mitochondria-Derived Activator of Caspase (DIABLO) Antibodies
Epitope AA 56-239
(38), (32), (13), (11), (8), (6), (4), (4), (4), (3), (2), (2), (2), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(184), (70), (56), (4), (4), (3), (3), (3), (3), (2), (1), (1)
Host Rabbit
(135), (53), (7), (1)
Clonality
Polyclonal
Conjugate This DIABLO antibody is un-conjugated
(2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), ELISA, Western Blotting (WB)
(182), (72), (66), (48), (31), (20), (9), (8), (7), (4), (4), (3), (2), (2), (1), (1), (1), (1), (1)
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Product Details anti-DIABLO Antibody

Target Details DIABLO Application Details Handling Images
Purpose Rabbit IgG polyclonal antibody for Diablo homolog, mitochondrial(DIABLO) detection. Tested with WB, IHC-P, ELISA in Human,Mouse,Rat.
Cross-Reactivity (Details) No cross reactivity with other proteins.
Characteristics Rabbit IgG polyclonal antibody for Diablo homolog, mitochondrial(DIABLO) detection. Tested with WB, IHC-P, ELISA in Human,Mouse,Rat.
Gene Name: diablo, IAP-binding mitochondrial protein
Protein Name: Diablo homolog, mitochondrial
Purification Immunogen affinity purified.
Immunogen E. coli-derived human Smac/Diablo recombinant protein (Position: A56-D239). Human Smac/Diablo shares 88.6% amino acid (aa) sequence identity with mouse Smac/Diablo.
Isotype IgG

Target Details DIABLO

Product Details anti-DIABLO Antibody Application Details Handling Images back to top
Antigen
Alternative Name DIABLO (DIABLO Antibody Abstract)
Background Diablo homolog (DIABLO), also referred to as second mitochondria-derived activator of caspases or SMAC, is a mitochondrial protein that in humans is encoded by the DIABLO (direct IAP binding protein with low pI) gene on chromosome 12. The encoded mitochondrial protein enters the cytosol when cells undergo apoptosis, and allows activation of caspases by binding to inhibitor of apoptosis proteins. Overexpression of the encoded protein sensitizes tumor cells to apoptosis. A mutation in this gene is associated with young-adult onset of nonsyndromic deafness-64. Alternative splicing results in multiple transcript variants encoding different isoforms.

Synonyms: 0610041G12Rik antibody|DBLOH_HUMAN antibody|DBOH antibody|diablo antibody|Diablo homolog (Drosophila) antibody|Diablo homolog antibody|Diablo homolog mitochondrial antibody|DIABLO S antibody|Direct IAP binding protein with low pI antibody|Direct IAP-binding protein with low pI antibody|FLJ10537 antibody|FLJ25049 antibody|mitochondrial antibody|Mitochondrial Smac protein antibody|Second mitochondria derived activator of caspase antibody|Second mitochondria-derived activator of caspase antibody|SMAC 3 antibody|Smac antibody|Smac protein antibody|SMAC3 antibody
Gene ID 56616
Research Area Apoptosis/Necrosis, DNA/RNA
Pathways Apoptosis, Caspase Cascade in Apoptosis, Positive Regulation of Endopeptidase Activity

Application Details

Product Details anti-DIABLO Antibody Target Details DIABLO Handling Images back to top
Application Notes WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Rat
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Mouse, Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
ELISA: Concentration: 0.1-0.5 μg/mL, Tested Species: Human

Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.
Comment

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions For Research Use only

Handling

Product Details anti-DIABLO Antibody Target Details DIABLO Application Details Images back to top
Format Lyophilized
Reconstitution Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
Concentration 500 μg/mL
Buffer Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Images

Product Details anti-DIABLO Antibody Target Details DIABLO Application Details Handling back to top
Supplier Images
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-DIABLO antibody (Second Mitochondria-Derived Activator of Caspase) (AA 56-239) (ABIN3044515) Smac/Diablo was detected in paraffin-embedded sections of human testis tissues using ...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-DIABLO antibody (Second Mitochondria-Derived Activator of Caspase) (AA 56-239) (ABIN3044515) Smac/Diablo was detected in paraffin-embedded sections of rat testis tissues using ra...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-DIABLO antibody (Second Mitochondria-Derived Activator of Caspase) (AA 56-239) (ABIN3044515) Smac/Diablo was detected in paraffin-embedded sections of mouse testis tissues using ...
Western Blotting (WB) image for anti-DIABLO antibody (Second Mitochondria-Derived Activator of Caspase) (AA 56-239) (ABIN3044515) Western blot analysis of Smac/Diablo expression in rat testis extract (lane 1), 22RV1...