You are viewing an incomplete version of our website. Please click to reload the website as full version.

anti-Human DIABLO antibody for Immunoprecipitation

Recommended DIABLO Antibody (supplied by: Log in to see )

Antigen
Second Mitochondria-Derived Activator of Caspase (DIABLO) Antibodies
  • 0610041G12Rik
  • 1700006L01Rik
  • AU040403
  • DBO
  • DFNA64
  • diablo-s
  • SMAC
  • Smac
  • smac
  • smac3
  • XSmac
Epitope
AA 1-55
32
16
13
11
8
6
4
4
4
3
3
2
2
2
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
139
55
35
4
4
3
2
1
1
Host
Mouse
118
30
8
1
Clonality
Monoclonal
Conjugate
This DIABLO antibody is un-conjugated
1
1
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
145
58
57
32
23
11
8
6
4
4
2
2
2
1
1
1
1
1
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

It's quick and easy to submit your validation proposal. I want to validate this product

Learn more

Catalog No. ABIN446307
Contact our Customer Service for availability and price in your country.
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.00089235196 ABIN957206 IHC (fro) IHC (p) IP WB Rabbit AA 56-73 Log in to see Polyclonal
0.00089235196 ABIN189310 ICC FACS IF IHC (p) IP IHC WB Rabbit IgG C-Term Log in to see Y12
0.00089235196 ABIN957207 IHC (fro) IHC (p) IP WB Rabbit Log in to see Polyclonal
0.00089235196 ABIN957208 IHC (fro) IHC (p) IP WB Rabbit Log in to see Polyclonal
0.00089235196 ABIN1996481 IP ELISA WB Rabbit IgG AA 56-239 Log in to see Polyclonal
0.00089235196 ABIN187400 IF IP WB Rat IgG2a Log in to see 10G7
0.00089235196 ABIN352289 IP WB Mouse IgG3 AA 1-55 Log in to see
0.00089235196 ABIN1585714 IP IHC ELISA WB Rabbit C-Term Log in to see Polyclonal
0.00089235196 ABIN218658 ICC IF IHC (p) IP ELISA WB Mouse IgG1 Log in to see
0.00089235196 ABIN252164 IHC (fro) IHC (p) IP IHC WB Rabbit IgG Log in to see Polyclonal
0.00089235196 ABIN352284 IHC (p) IP WB Rabbit IgG Log in to see Polyclonal
0.00089235196 ABIN2362660 IP IHC WB Rabbit IgG AA 56-73 Log in to see Polyclonal
0.00089235196 ABIN2505678 ICC FACS IP ELISA WB Rat IgG2a AA 55-237 Log in to see 10G7
0.00089235196 ABIN2167846 IP ELISA WB Rabbit AA 56-239, His6 Log in to see Polyclonal
0.00089235196 ABIN2362647 IP WB Mouse IgG3, IgG1 Log in to see 9E706
0.00089235196 ABIN2362658 IC IF IP IHC WB Mouse IgG1 Log in to see 4i16
0.00089235196 ABIN2362664 IP IHC WB Rabbit IgG Log in to see Polyclonal
0.00089235196 ABIN2362645 IP IHC WB Rabbit IgG Log in to see Polyclonal
0.00089235196 ABIN2362662 IP ELISA WB Rabbit AA 56-239 Log in to see Polyclonal
0.00089235196 ABIN199860 ICC FACS IP ELISA WB Rat IgG2a Log in to see

Top referenced anti-DIABLO antibody for Immunoprecipitation

  • Human Monoclonal DIABLO Primary Antibody for ICC, IF - ABIN446307 (2 Publications): Wang, Zhu, Drozda, Zhang, Stavrovskaya, Cattaneo, Ferrante, Kristal, Friedlander: Minocycline inhibits caspase-independent and -dependent mitochondrial cell death pathways in models of Huntington's disease. in Proceedings of the National Academy of Sciences of the United States of America 2003 (PubMed)

Similar anti-DIABLO Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine)
ELISA 16 Antibodies 24 Antibodies
Enzyme Immunoassay (EIA) 1 Antibodies 1 Antibodies
Flow Cytometry (FACS) 1 Antibodies 6 Antibodies
Immunocytochemistry (ICC) 3 Antibodies 6 Antibodies
Immunofluorescence (IF) 6 Antibodies 7 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 1 Antibodies 1 Antibodies
Immunohistochemistry (IHC) 23 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 10 Antibodies
Immunoprecipitation (IP) 3 Antibodies
Western Blotting (WB) 36 Antibodies
Immunochromatography (IC) 3 Antibodies

General

Antigen Second Mitochondria-Derived Activator of Caspase (DIABLO) Antibodies
Epitope AA 1-55
(32), (16), (13), (11), (8), (6), (4), (4), (4), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(139), (55), (35), (4), (4), (3), (2), (1), (1)
Host Mouse
(118), (30), (8), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This DIABLO antibody is un-conjugated
(1), (1)
Application Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
(145), (58), (57), (32), (23), (11), (8), (6), (4), (4), (2), (2), (2), (1), (1), (1), (1), (1)
Pubmed 2 references available
Supplier Log in to see

Product Details anti-DIABLO Antibody

Target Details DIABLO Application Details Handling References for anti-DIABLO Antibody (ABIN446307) Images
Purification Ascites
Immunogen Recombinant mature human Smac (lacks amino acids 1-55 present in Smac precursor).
Clone 78-1-118
Isotype IgG3, IgG1

Target Details DIABLO

Product Details anti-DIABLO Antibody Application Details Handling References for anti-DIABLO Antibody (ABIN446307) Images back to top
Antigen
Alternative Name SMAC/Diablo (DIABLO Antibody Abstract)
Background Smac/DIABLO, a mitochondrial protein, activates various forms of apoptosis. Thisactivation may be due to the neutralization of one or more members of the IAP family ofapoptosis inhibitory proteins. Smac exits the mitochondria and enter the cytosol duringcertain apoptosis triggered events. Mitochondrial-mediated apoptosis is important inanimal development and tissue homeostasis, with alterations resulting in a range ofmalignant disorders. Upon apoptotic stimuli, the mitochondrial proteins cytochrome cand Smac/DIABLO are released into the cytosol. The release of these proteins,however, occurs via different mechanisms (2,3). Alternate Names: anti-DIABLO-S antibody, anti-FLJ10537 antibody,, anti-FLJ25049 antibody, anti-SMACantibody, anti-SMAC3 antibody.
Gene Symbol: DIABLO
Gene ID 56616, 66593, 288753
UniProt Q9NR28
Pathways Apoptosis, Caspase Cascade in Apoptosis

Application Details

Product Details anti-DIABLO Antibody Target Details DIABLO Handling References for anti-DIABLO Antibody (ABIN446307) Images back to top
Application Notes This Smac (78-1-118) antibody is useful for Immunoprecipitation and Western Blot, where a band is seen at ~25kDa. For better results IP prior to Western blot is recommended.
Recommended dilutions: Immunocytochemistry/Immunofluorescence, Immunoprecipitation 1:10-1:500, Western Blot 1:500
Protocol Immunoprecipitation protocol specific for Smac Antibody Procedure Guide for NB 500-213 Monoclonal anti-Survivin (clone 58.22)Immunoprecipitation Procedure1) Lyse cells plated in a 60mm dish:a) 300 µL CHAPS buffer [50mM Tris-HCl, pH 7.5/50mM NaCl/1mM EDTA/1 % NP-40/0.1 % CHAPS/1mM NaVO4/1mM PMSF]b) rock for 20 minutes at 4C2) Harvest lysate and spin down the insoluble material at 14K rpm. 3) Collect soluble fraction.
4) Pre-clear lysate with 40 µL of 50:50 slurry of Protein A beads, rocking for 1 hour at 4C.
) Spin down beads at 2K rpm, at 4C.
) Collect pre-cleared lysate.
) Incubate lysate with 5-7ug of anti-Survivin overnight, rocking at 4C. 8) Add 50 µL of Protein A 50:50 slurry for 2 hours, rocking at 4C.
) Wash beads with 200 µL of CHAPS buffer, three times.
) Denature immune complex by adding 2x Sample
Restrictions For Research Use only

Handling

Product Details anti-DIABLO Antibody Target Details DIABLO Application Details References for anti-DIABLO Antibody (ABIN446307) Images back to top
Format Liquid
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid freeze-thaw cycles
Storage -20 °C
Storage Comment Aliquot and store at -20 °C or -80 °C.

References for anti-DIABLO Antibody (ABIN446307)

Product Details anti-DIABLO Antibody Target Details DIABLO Application Details Handling Images back to top
Product cited in:

Wang, Zhu, Drozda, Zhang, Stavrovskaya, Cattaneo, Ferrante, Kristal, Friedlander: "Minocycline inhibits caspase-independent and -dependent mitochondrial cell death pathways in models of Huntington's disease." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 100, Issue 18, pp. 10483-7, 2003

Zhang, Wang, Narayanan, Zhang, Huo, Reed, Friedlander: "Fundamental role of the Rip2/caspase-1 pathway in hypoxia and ischemia-induced neuronal cell death." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 100, Issue 26, pp. 16012-7, 2003