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anti-Mouse (Murine) DIABLO antibody for Enzyme Immunoassay

Recommended DIABLO Antibody (supplied by: Log in to see )

Antigen
Second Mitochondria-Derived Activator of Caspase (DIABLO) Antibodies
  • 0610041G12Rik
  • 1700006L01Rik
  • AU040403
  • DBO
  • DFNA64
  • diablo-s
  • SMAC
  • Smac
  • smac
  • smac3
  • XSmac
Epitope
AA 222-237, C-Term
35
31
12
11
8
6
5
4
4
3
3
2
2
2
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
169
61
41
4
4
3
2
1
1
1
Host
Rabbit
128
50
9
1
Clonality
Polyclonal
Conjugate
This DIABLO antibody is un-conjugated
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
176
78
67
43
30
16
11
9
6
4
2
2
1
1
1
1
1
1
1
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Catalog No. ABIN500784
$ 401.50
Plus shipping costs $45.00

Top referenced anti-DIABLO antibody for Enzyme Immunoassay

Similar anti-DIABLO Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine)
ELISA 17 Antibodies 26 Antibodies
Enzyme Immunoassay (EIA) 1 Antibodies 1 Antibodies
Flow Cytometry (FACS) 1 Antibodies 7 Antibodies
Immunocytochemistry (ICC) 5 Antibodies 8 Antibodies
Immunofluorescence (IF) 8 Antibodies 9 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 1 Antibodies 1 Antibodies
Immunohistochemistry (IHC) 29 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 16 Antibodies
Immunoprecipitation (IP) 6 Antibodies
Western Blotting (WB) 42 Antibodies
Immunochromatography (IC) 3 Antibodies

General

Antigen Second Mitochondria-Derived Activator of Caspase (DIABLO) Antibodies
Epitope AA 222-237, C-Term
(35), (31), (12), (11), (8), (6), (5), (4), (4), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(169), (61), (41), (4), (4), (3), (2), (1), (1), (1)
Host Rabbit
(128), (50), (9), (1)
Clonality
Polyclonal
Conjugate This DIABLO antibody is un-conjugated
(2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(176), (78), (67), (43), (30), (16), (11), (9), (6), (4), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Pubmed 1 reference available
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Product Details anti-DIABLO Antibody

Target Details DIABLO Application Details Handling References for anti-DIABLO Antibody (ABIN500784) Images
Specificity This antibody detects DIABLO / SMAC at C-term.
Cross-Reactivity (Details) Species reactivity (tested):Human, mouse, rat
Purification Peptide affinity chromatography
Immunogen Smac antibody was raised against a peptide corresponding to amino acids 222 to 237 of murine Smac/DIABLO.
Isotype IgG

Target Details DIABLO

Product Details anti-DIABLO Antibody Application Details Handling References for anti-DIABLO Antibody (ABIN500784) Images back to top
Antigen
Alternative Name DIABLO / SMAC (DIABLO Antibody Abstract)
Background The inhibitor of apoptosis proteins (IAPs) regulate programmed cell death by inhibiting members of the caspase family of enzymes. A novel mammalian protein that binds to IAPs and neutralizes the inhibitory effect of IAPs on caspases was recently identified and designated Smac/DIABLO (1,2). Smac/DIABLO is a mitochondrial protein that is released along with cytochrome c during apoptosis and activates cytochrome c/Apaf-1/capase-9 pathway. Analysis of the structural basis of Smac/DIABLO reveals that the N-terminal amino acids are required for binding of Smac/DIABLO to IAPs and activation of caspases (3-6). Smac/DIABLO is expressed in a variety of human and mouse tissues (1,2).Synonyms: Diablo homolog mitochondrial, Direct IAP-binding protein with low pI, Second mitochondria-derived activator of caspase
Gene ID 66593
UniProt Q9JIQ3
Pathways Apoptosis, Caspase Cascade in Apoptosis

Application Details

Product Details anti-DIABLO Antibody Target Details DIABLO Handling References for anti-DIABLO Antibody (ABIN500784) Images back to top
Application Notes ELISA. Western blot: 0.5 to 1 μg/mL, 25 kDa band can be detected. Immunohistochemistry on paraffin sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-DIABLO Antibody Target Details DIABLO Application Details References for anti-DIABLO Antibody (ABIN500784) Images back to top
Buffer PBS containing 0.02 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.

References for anti-DIABLO Antibody (ABIN500784)

Product Details anti-DIABLO Antibody Target Details DIABLO Application Details Handling Images back to top
Background publications

Du, Fang, Li, Li, Wang: "Smac, a mitochondrial protein that promotes cytochrome c-dependent caspase activation by eliminating IAP inhibition." in: Cell, Vol. 102, Issue 1, pp. 33-42, 2000

Images

Product Details anti-DIABLO Antibody Target Details DIABLO Application Details Handling References for anti-DIABLO Antibody (ABIN500784) back to top
Supplier Images
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-DIABLO antibody (Second Mitochondria-Derived Activator of Caspase) (AA 222-237) (ABIN500784) Immunohistochemical staining of mouse spleen tissue using AP30815PU-N at 2 μg/ml.
Western Blotting (WB) image for anti-DIABLO antibody (Second Mitochondria-Derived Activator of Caspase) (AA 222-237) (ABIN500784) Western blot analysis of Smac in mouse heart tissue lysate in the absence (A) or pres...