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anti-Mouse (Murine) DIABLO antibody for ELISA

Recommended DIABLO Antibody (supplied by: Log in to see )

Antigen
Second Mitochondria-Derived Activator of Caspase (DIABLO) Antibodies
  • 0610041G12Rik
  • 1700006L01Rik
  • AU040403
  • DBO
  • DFNA64
  • diablo-s
  • SMAC
  • Smac
  • smac
  • smac3
  • XSmac
Epitope
AA 56-239
34
32
13
11
8
6
5
4
4
3
3
2
2
2
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
167
59
39
4
4
3
2
1
1
Host
Rabbit
127
49
9
1
Clonality
Polyclonal
Conjugate
This DIABLO antibody is un-conjugated
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), ELISA, Western Blotting (WB)
174
77
66
42
28
15
10
9
5
4
2
2
2
1
1
1
1
1
Supplier
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Catalog No. ABIN3044515
$ 240.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0012121403 ABIN1820692 IHC ELISA WB Rabbit IgG C-Term Log in to see Polyclonal
0.0012121403 ABIN1585714 IP IHC ELISA WB Rabbit C-Term Log in to see Polyclonal
0.0012121403 ABIN2428719 IHC ELISA WB Rabbit IgG Log in to see Polyclonal
0.0012121403 ABIN2422157 IHC ELISA WB Rabbit IgG Log in to see Polyclonal
0.0012121403 ABIN1085183 IHC ELISA WB Rabbit IgG Log in to see Polyclonal
0.0012121403 ABIN1085184 IHC ELISA WB Rabbit IgG Log in to see Polyclonal
0.0012121403 ABIN1030686 IHC ELISA WB Rabbit C-Term Log in to see Polyclonal
0.0012121403 ABIN1030687 IHC ELISA WB Rabbit C-Term Log in to see Polyclonal
0.0012121403 ABIN2505677 ICC FACS IP IHC ELISA WB Rat IgG2a AA 55-237 Log in to see 9H10
0.0012121403 ABIN2505678 ICC FACS IP ELISA WB Rat IgG2a AA 55-237 Log in to see 10G7
0.0012121403 ABIN2362643 Neut ELISA WB Rabbit IgG AA 222-237, C-Term Log in to see Polyclonal
0.0012121403 ABIN2362652 IC FACS IP ELISA WB Rat IgG2a AA 55-237 Log in to see 6D766
0.0012121403 ABIN2362653 IC FACS IP IHC ELISA WB Rat IgG2a AA 55-237 Log in to see 6D767
0.0012121403 ABIN2505676 IHC ELISA WB Rabbit AA 225-239, N-Term Log in to see Polyclonal
0.0012121403 ABIN2505675 IHC ELISA WB Rabbit AA 222-237, N-Term Log in to see Polyclonal
0.0012121403 ABIN1886858 IHC ELISA WB Rabbit AA 222-237 Log in to see Polyclonal
0.0012121403 ABIN1886859 IHC ELISA WB Rabbit AA 225-239 Log in to see Polyclonal
0.0012121403 ABIN2362642 IHC ELISA WB Rabbit IgG C-Term Log in to see Polyclonal
0.0012121403 ABIN4354647 ELISA FACS ICC IF IHC IHC (fro) IHC (p) IP WB Rat IgG2a AA 55-237 Log in to see 9H10
0.0012121403 ABIN1585254 IHC ELISA WB Rabbit Log in to see Polyclonal

Similar anti-DIABLO Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine)
ELISA 17 Antibodies 26 Antibodies
Enzyme Immunoassay (EIA) 1 Antibodies 1 Antibodies
Flow Cytometry (FACS) 1 Antibodies 7 Antibodies
Immunocytochemistry (ICC) 5 Antibodies 8 Antibodies
Immunofluorescence (IF) 8 Antibodies 9 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 1 Antibodies 1 Antibodies
Immunohistochemistry (IHC) 28 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 15 Antibodies
Immunoprecipitation (IP) 5 Antibodies
Western Blotting (WB) 41 Antibodies
Immunochromatography (IC) 3 Antibodies

General

Antigen Second Mitochondria-Derived Activator of Caspase (DIABLO) Antibodies
Epitope AA 56-239
(34), (32), (13), (11), (8), (6), (5), (4), (4), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(167), (59), (39), (4), (4), (3), (2), (1), (1)
Host Rabbit
(127), (49), (9), (1)
Clonality
Polyclonal
Conjugate This DIABLO antibody is un-conjugated
(2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), ELISA, Western Blotting (WB)
(174), (77), (66), (42), (28), (15), (10), (9), (5), (4), (2), (2), (2), (1), (1), (1), (1), (1)
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Product Details anti-DIABLO Antibody

Target Details DIABLO Application Details Handling Images
Cross-Reactivity (Details) No cross reactivity with other proteins.
Characteristics Rabbit IgG polyclonal antibody for Diablo homolog, mitochondrial(DIABLO) detection. Tested with WB, IHC-P, ELISA in Human,Mouse,Rat.
Gene Name: diablo, IAP-binding mitochondrial protein
Protein Name: Diablo homolog, mitochondrial
Purification Immunogen affinity purified.
Immunogen E. coli-derived human Smac/Diablo recombinant protein (Position: A56-D239). Human Smac/Diablo shares 88.6% amino acid (aa) sequence identity with human Smac/Diablo.
Isotype IgG

Target Details DIABLO

Product Details anti-DIABLO Antibody Application Details Handling Images back to top
Antigen
Alternative Name DIABLO (DIABLO Antibody Abstract)
Background Diablo homolog (DIABLO), also referred to as second mitochondria-derived activator of caspases or SMAC, is a mitochondrial protein that in humans is encoded by the DIABLO (direct IAP binding protein with low pI) gene on chromosome 12. The encoded mitochondrial protein enters the cytosol when cells undergo apoptosis, and allows activation of caspases by binding to inhibitor of apoptosis proteins. Overexpression of the encoded protein sensitizes tumor cells to apoptosis. A mutation in this gene is associated with young-adult onset of nonsyndromic deafness-64. Alternative splicing results in multiple transcript variants encoding different isoforms.
Gene ID 56616
Research Area Apoptosis/Necrosis, DNA/RNA
Pathways Apoptosis, Caspase Cascade in Apoptosis

Application Details

Product Details anti-DIABLO Antibody Target Details DIABLO Handling Images back to top
Application Notes WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Rat
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Mouse, Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
ELISA: Concentration: 0.1-0.5 μg/mL, Tested Species: Human
Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.
Comment

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions For Research Use only

Handling

Product Details anti-DIABLO Antibody Target Details DIABLO Application Details Images back to top
Format Lyophilized
Reconstitution Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
Concentration 500 μg/mL
Buffer Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C,4 °C
Storage Comment At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Images

Product Details anti-DIABLO Antibody Target Details DIABLO Application Details Handling back to top
Supplier Images
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-DIABLO antibody (Second Mitochondria-Derived Activator of Caspase) (AA 56-239) (ABIN3044515) Smac/Diablo was detected in paraffin-embedded sections of human testis tissues using ...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-DIABLO antibody (Second Mitochondria-Derived Activator of Caspase) (AA 56-239) (ABIN3044515) Smac/Diablo was detected in paraffin-embedded sections of rat testis tissues using ra...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-DIABLO antibody (Second Mitochondria-Derived Activator of Caspase) (AA 56-239) (ABIN3044515) Smac/Diablo was detected in paraffin-embedded sections of mouse testis tissues using ...
Western Blotting (WB) image for anti-DIABLO antibody (Second Mitochondria-Derived Activator of Caspase) (AA 56-239) (ABIN3044515) Western blot analysis of Smac/Diablo expression in rat testis extract (lane 1), 22RV1...