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|Application / Reactivity||Rat (Rattus)||Mouse (Murine)|
|ELISA||18 Antibodies||27 Antibodies|
|Enzyme Immunoassay (EIA)||1 Antibodies||1 Antibodies|
|Immunochromatography (IC)||1 Antibodies||3 Antibodies|
|Immunocytochemistry (ICC)||4 Antibodies||7 Antibodies|
|Immunofluorescence (IF)||8 Antibodies||9 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||1 Antibodies|
|Immunohistochemistry (IHC)||29 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||1 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||15 Antibodies|
|Immunoprecipitation (IP)||7 Antibodies|
|Simple Western (SimWes)||1 Antibodies|
|Western Blotting (WB)||42 Antibodies|
|Flow Cytometry (FACS)||6 Antibodies|
|Antigen||Second Mitochondria-Derived Activator of Caspase (DIABLO) Antibodies|
|Epitope||AA 56-239 Alternatives|
|Reactivity||Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This DIABLO antibody is un-conjugated Alternatives|
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), ELISA, Western Blotting (WB)
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Product Details anti-DIABLO AntibodyTarget Details DIABLO Application Details Handling Images
|Cross-Reactivity (Details)||No cross reactivity with other proteins.|
Rabbit IgG polyclonal antibody for Diablo homolog, mitochondrial(DIABLO) detection. Tested with WB, IHC-P, ELISA in Human,Mouse,Rat.
Gene Name: diablo, IAP-binding mitochondrial protein
Protein Name: Diablo homolog, mitochondrial
|Purification||Immunogen affinity purified.|
|Immunogen||E. coli-derived human Smac/Diablo recombinant protein (Position: A56-D239). Human Smac/Diablo shares 88.6% amino acid (aa) sequence identity with human Smac/Diablo.|
Target Details DIABLOProduct Details anti-DIABLO Antibody Application Details Handling Images back to top
|Alternative Name||DIABLO (DIABLO Antibody Abstract)|
|Background||Diablo homolog (DIABLO), also referred to as second mitochondria-derived activator of caspases or SMAC, is a mitochondrial protein that in humans is encoded by the DIABLO (direct IAP binding protein with low pI) gene on chromosome 12. The encoded mitochondrial protein enters the cytosol when cells undergo apoptosis, and allows activation of caspases by binding to inhibitor of apoptosis proteins. Overexpression of the encoded protein sensitizes tumor cells to apoptosis. A mutation in this gene is associated with young-adult onset of nonsyndromic deafness-64. Alternative splicing results in multiple transcript variants encoding different isoforms.|
|Research Area||Apoptosis/Necrosis, DNA/RNA|
|Pathways||Apoptosis, Caspase Cascade in Apoptosis|
Application DetailsProduct Details anti-DIABLO Antibody Target Details DIABLO Handling Images back to top
WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Rat
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Mouse, Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
ELISA: Concentration: 0.1-0.5 μg/mL, Tested Species: Human
Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.
Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).
|Restrictions||For Research Use only|
HandlingProduct Details anti-DIABLO Antibody Target Details DIABLO Application Details Images back to top
|Reconstitution||Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.|
|Buffer||Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.|
|Precaution of Use||This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Storage||-20 °C,4 °C|
At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.
ImagesProduct Details anti-DIABLO Antibody Target Details DIABLO Application Details Handling back to top