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anti-Mouse (Murine) DIABLO antibody for ELISA

Recommended DIABLO Antibody (supplied by: Log in to see )

Antigen
Second Mitochondria-Derived Activator of Caspase (DIABLO) Antibodies
  • smac
  • smac3
  • diablo-s
  • 0610041G12Rik
  • 1700006L01Rik
  • AU040403
  • Smac
  • DFNA64
  • SMAC
  • DBO
  • XSmac
  • diablo, IAP-binding mitochondrial protein
  • diablo homolog (Drosophila)
  • diablo
  • Diablo
  • DIABLO
Epitope
AA 56-239
35
34
14
12
8
6
5
4
4
4
3
2
2
2
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
174
63
43
4
4
3
2
1
1
1
Host
Rabbit
133
50
9
1
Clonality
Polyclonal
Conjugate
This DIABLO antibody is un-conjugated
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), ELISA, Western Blotting (WB)
178
78
69
44
32
18
12
10
6
4
2
2
2
1
1
1
1
1
1
Options
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Catalog No. ABIN3044515
$ 240.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN4354647 ELISA FACS ICC IF IHC IHC (fro) IHC (p) IP WB Rat IgG2a AA 55-237 Log in to see 9H10 3
1 ABIN1585714 IP IHC ELISA WB Rabbit C-Term Log in to see Polyclonal
1 ABIN4354645 ELISA ICC IF IHC IHC (p) IP WB Rabbit Log in to see Polyclonal 1
1 ABIN1820692 IHC ELISA WB Rabbit IgG C-Term Log in to see Polyclonal
1 ABIN2428719 IHC ELISA WB Rabbit IgG Log in to see Polyclonal
1 ABIN2422157 IHC ELISA WB Rabbit IgG Log in to see Polyclonal
1 ABIN1085184 IHC ELISA WB Rabbit IgG Log in to see Polyclonal
1 ABIN1085183 IHC ELISA WB Rabbit IgG Log in to see Polyclonal
1 ABIN1030686 IHC ELISA WB Rabbit C-Term Log in to see Polyclonal
1 ABIN1030687 IHC ELISA WB Rabbit C-Term Log in to see Polyclonal
1 ABIN2505678 ICC FACS IP ELISA WB Rat IgG2a AA 55-237 Log in to see 10G7
1 ABIN2505677 ICC FACS IP IHC ELISA WB Rat IgG2a AA 55-237 Log in to see 9H10
1 ABIN2362643 Neut ELISA WB Rabbit IgG AA 222-237, C-Term Log in to see Polyclonal
1 ABIN2362653 IC FACS IP IHC ELISA WB Rat IgG2a AA 55-237 Log in to see 6D767
1 ABIN2362652 IC FACS IP ELISA WB Rat IgG2a AA 55-237 Log in to see 6D766
1 ABIN2505676 IHC ELISA WB Rabbit AA 225-239, N-Term Log in to see Polyclonal
1 ABIN2505675 IHC ELISA WB Rabbit AA 222-237, N-Term Log in to see Polyclonal
1 ABIN4354644 ELISA IHC IHC (p) IP WB Rabbit Log in to see Polyclonal
1 ABIN2362642 IHC ELISA WB Rabbit IgG C-Term Log in to see Polyclonal
1 ABIN1886858 IHC ELISA WB Rabbit AA 222-237 Log in to see Polyclonal

Top referenced anti-DIABLO antibody for ELISA

Similar anti-DIABLO Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine)
ELISA 18 Antibodies 27 Antibodies
Enzyme Immunoassay (EIA) 1 Antibodies 1 Antibodies
Immunochromatography (IC) 1 Antibodies 3 Antibodies
Immunocytochemistry (ICC) 4 Antibodies 7 Antibodies
Immunofluorescence (IF) 8 Antibodies 9 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 1 Antibodies
Immunohistochemistry (IHC) 29 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 15 Antibodies
Immunoprecipitation (IP) 7 Antibodies
Simple Western (SimWes) 1 Antibodies
Western Blotting (WB) 42 Antibodies
Flow Cytometry (FACS) 6 Antibodies

General

Antigen Second Mitochondria-Derived Activator of Caspase (DIABLO) Antibodies
Epitope AA 56-239
(35), (34), (14), (12), (8), (6), (5), (4), (4), (4), (3), (2), (2), (2), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(174), (63), (43), (4), (4), (3), (2), (1), (1), (1)
Host Rabbit
(133), (50), (9), (1)
Clonality
Polyclonal
Conjugate This DIABLO antibody is un-conjugated
(2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), ELISA, Western Blotting (WB)
(178), (78), (69), (44), (32), (18), (12), (10), (6), (4), (2), (2), (2), (1), (1), (1), (1), (1), (1)
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Product Details anti-DIABLO Antibody

Target Details DIABLO Application Details Handling Images
Cross-Reactivity (Details) No cross reactivity with other proteins.
Characteristics Rabbit IgG polyclonal antibody for Diablo homolog, mitochondrial(DIABLO) detection. Tested with WB, IHC-P, ELISA in Human,Mouse,Rat.
Gene Name: diablo, IAP-binding mitochondrial protein
Protein Name: Diablo homolog, mitochondrial
Purification Immunogen affinity purified.
Immunogen E. coli-derived human Smac/Diablo recombinant protein (Position: A56-D239). Human Smac/Diablo shares 88.6% amino acid (aa) sequence identity with human Smac/Diablo.
Isotype IgG

Target Details DIABLO

Product Details anti-DIABLO Antibody Application Details Handling Images back to top
Antigen
Alternative Name DIABLO (DIABLO Antibody Abstract)
Background Diablo homolog (DIABLO), also referred to as second mitochondria-derived activator of caspases or SMAC, is a mitochondrial protein that in humans is encoded by the DIABLO (direct IAP binding protein with low pI) gene on chromosome 12. The encoded mitochondrial protein enters the cytosol when cells undergo apoptosis, and allows activation of caspases by binding to inhibitor of apoptosis proteins. Overexpression of the encoded protein sensitizes tumor cells to apoptosis. A mutation in this gene is associated with young-adult onset of nonsyndromic deafness-64. Alternative splicing results in multiple transcript variants encoding different isoforms.
Gene ID 56616
Research Area Apoptosis/Necrosis, DNA/RNA
Pathways Apoptosis, Caspase Cascade in Apoptosis

Application Details

Product Details anti-DIABLO Antibody Target Details DIABLO Handling Images back to top
Application Notes WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Rat
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Mouse, Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
ELISA: Concentration: 0.1-0.5 μg/mL, Tested Species: Human
Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.
Comment

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions For Research Use only

Handling

Product Details anti-DIABLO Antibody Target Details DIABLO Application Details Images back to top
Format Lyophilized
Reconstitution Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
Concentration 500 μg/mL
Buffer Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C,4 °C
Storage Comment At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Images

Product Details anti-DIABLO Antibody Target Details DIABLO Application Details Handling back to top
Supplier Images
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-DIABLO antibody (Second Mitochondria-Derived Activator of Caspase) (AA 56-239) (ABIN3044515) Smac/Diablo was detected in paraffin-embedded sections of human testis tissues using ...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-DIABLO antibody (Second Mitochondria-Derived Activator of Caspase) (AA 56-239) (ABIN3044515) Smac/Diablo was detected in paraffin-embedded sections of rat testis tissues using ra...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-DIABLO antibody (Second Mitochondria-Derived Activator of Caspase) (AA 56-239) (ABIN3044515) Smac/Diablo was detected in paraffin-embedded sections of mouse testis tissues using ...
Western Blotting (WB) image for anti-DIABLO antibody (Second Mitochondria-Derived Activator of Caspase) (AA 56-239) (ABIN3044515) Western blot analysis of Smac/Diablo expression in rat testis extract (lane 1), 22RV1...