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anti-Mouse (Murine) DIABLO antibody for Flow Cytometry

Recommended DIABLO Antibody (supplied by: Log in to see )

Antigen
Second Mitochondria-Derived Activator of Caspase (DIABLO) Antibodies
  • 0610041G12Rik
  • 1700006L01Rik
  • AU040403
  • DBO
  • DFNA64
  • diablo-s
  • SMAC
  • Smac
  • smac
  • smac3
  • XSmac
Epitope
C-Term
32
16
13
11
8
6
4
4
4
3
3
2
2
2
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
139
55
35
4
4
3
2
1
1
Host
Rabbit
118
30
8
1
Clonality
Monoclonal
Conjugate
This DIABLO antibody is un-conjugated
1
1
Application
Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
145
58
57
32
23
11
8
6
4
4
2
2
2
1
1
1
1
1
Supplier
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Available images

Catalog No. ABIN189310
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0010666461 ABIN2505677 ICC FACS IP IHC ELISA WB Rat IgG2a AA 55-237 Log in to see 9H10
0.0010666461 ABIN2505678 ICC FACS IP ELISA WB Rat IgG2a AA 55-237 Log in to see 10G7
0.0010666461 ABIN2362652 IC FACS IP ELISA WB Rat IgG2a AA 55-237 Log in to see 6D766
0.0010666461 ABIN2362653 IC FACS IP IHC ELISA WB Rat IgG2a AA 55-237 Log in to see 6D767
0.0010666461 ABIN199861 ICC FACS IP IHC ELISA WB Rat IgG2a Log in to see

Similar anti-DIABLO Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine)
ELISA 16 Antibodies 24 Antibodies
Enzyme Immunoassay (EIA) 1 Antibodies 1 Antibodies
Flow Cytometry (FACS) 1 Antibodies 6 Antibodies
Immunocytochemistry (ICC) 3 Antibodies 6 Antibodies
Immunofluorescence (IF) 6 Antibodies 7 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 1 Antibodies 1 Antibodies
Immunohistochemistry (IHC) 23 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 10 Antibodies
Immunoprecipitation (IP) 3 Antibodies
Western Blotting (WB) 36 Antibodies
Immunochromatography (IC) 3 Antibodies

General

Antigen Second Mitochondria-Derived Activator of Caspase (DIABLO) Antibodies
Epitope C-Term
(32), (16), (13), (11), (8), (6), (4), (4), (4), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(139), (55), (35), (4), (4), (3), (2), (1), (1)
Host Rabbit
(118), (30), (8), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This DIABLO antibody is un-conjugated
(1), (1)
Application Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
(145), (58), (57), (32), (23), (11), (8), (6), (4), (4), (2), (2), (2), (1), (1), (1), (1), (1)
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Product Details anti-DIABLO Antibody

Target Details DIABLO Application Details Handling Images
Purification Protein A purified
Immunogen A synthetic peptide corresponding to C-terminal residues human Smac/DIABLO was used as immunogen.
Clone Y12
Isotype IgG

Target Details DIABLO

Product Details anti-DIABLO Antibody Application Details Handling Images back to top
Antigen
Alternative Name DIABLO / SMAC (DIABLO Antibody Abstract)
Background Smac/Diablo (Second Mitochondria-derived Activator of Caspases/Direct IAP BindingProtein with Low PI) is localized to the mitochondria and involved in activatingprogrammed cell death, or apoptosis (1). In response to apoptotic stimuli, Smac/Diablois released from the mitochondria and binds anti-apoptotic IAP proteins, suppressingtheir inhibitory activity and promoting caspase activation (1,2). This interaction involvesprimarily the amino-terminal residues of Smac/Diablo with the BIR3 region of the IAPprotein (3,4). Alternate Names: anti-0610041G12Rik antibody, anti-DBOH antibody, anti-Diablo homolog (Drosophila)antibody, anti-Diablo homolog antibody, anti-Diablo homolog mitochondrial antibody,anti-DIABLO S antibody, anti-Direct IAP binding protein with low pI antibody,anti-FLJ10537 antibody, anti-FLJ25049 antibody, anti-Mitochondrial Smac proteinantibody, anti-Second mitochondria derived activator of caspase antibody, anti-SMAC 3antibody, anti-Smac protein antibody, anti-SMAC3 antibody.
Gene Symbol: DIABLO
Gene ID 56616, 66593, 288753
Pathways Apoptosis, Caspase Cascade in Apoptosis

Application Details

Product Details anti-DIABLO Antibody Target Details DIABLO Handling Images back to top
Application Notes This product is useful for: Western Blot, Immunohistochemistry-Paraffin, Immunocytochemistry, Immunoprecipitation, Flow Cytometry. In Western blot this antibody detects a band at approximately 21/27kDa.
Recommended dilutions: Flow Cytometry 1:10-50, Immunocytochemistry/Immunofluorescence 1:10, Immunohistochemistry 1:10-1:500, Immunohistochemistry-Paraffin 1:10, Immunoprecipitation 1:10-80, Western Blot 1:1000
Protocol Protocol specific for Smac Antibody Immunohistochemistry Protocol for Paraffin-embedded Tissues
1. Solutions and reagents1.
. Xylene1.
. Ethanol, anhydrous denatured, histological grade (100 %, 95 %, 70 %)1.
. Washing buffer:TBST washing buffer: 1XTBS/0.1 % Tween-20To prepare stock solution of 10X TBS: add 24.2 g Trizma base and 80 g sodium chloride to 1L of dH2O. Adjust pH to 7.6.Working solution. 1XTBST/0.1 % Tween-20: add 100ml 10XTBS to 900 mL dH2O. Add 1 mL Tween-20 and mix well.1.
. Distilled water (dH2O)1.
. Antigen Retrieval Solution:0.01M Sodium Citrate
Restrictions For Research Use only

Handling

Product Details anti-DIABLO Antibody Target Details DIABLO Application Details Images back to top
Format Liquid
Buffer PBS 49 % , Glycerol 50 % , BSA 0.05 % , Sodium Azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid freeze-thaw cycles
Storage -20 °C
Storage Comment -20 °C.

Images

Product Details anti-DIABLO Antibody Target Details DIABLO Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-DIABLO antibody (Second Mitochondria-Derived Activator of Caspase) (C-Term) (ABIN189310) Western blot analysis on Jurkat cell lysate using anti-Smac/Diablo RabMAb (catalog #1...
Immunohistochemistry (IHC) image for anti-DIABLO antibody (Second Mitochondria-Derived Activator of Caspase) (C-Term) (ABIN189310) Immunohistochemical analysis of paraffin-embedded human skin cancer tissue using anti...