anti-Mouse (Murine) DIABLO antibody for Immunoprecipitation

Recommended DIABLO Antibody (supplied by: Log in to see )

Antigen
Second Mitochondria-Derived Activator of Caspase (DIABLO) Antibodies
  • smac
  • smac3
  • diablo-s
  • 0610041G12Rik
  • 1700006L01Rik
  • AU040403
  • Smac
  • DFNA64
  • SMAC
  • DBO
  • XSmac
  • diablo, IAP-binding mitochondrial protein
  • diablo homolog (Drosophila)
  • diablo
  • Diablo
  • DIABLO
Reactivity
Human, Mouse (Murine), Rat (Rattus)
185
70
56
4
4
3
3
3
3
2
1
1
Host
Mouse
137
52
7
1
Clonality
Monoclonal
Conjugate
This DIABLO antibody is un-conjugated
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
183
73
66
50
30
19
8
8
7
4
4
3
2
2
1
1
1
1
1
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Catalog No. ABIN153019
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN4354647 ELISA FACS ICC IF IHC IHC (fro) IHC (p) IP WB Rat IgG2a AA 55-237 Log in to see 9H10 3
1 ABIN4354645 ELISA ICC IF IHC IHC (p) IP WB Rabbit C-Term Log in to see Polyclonal 1
1 ABIN4354640 IHC IHC (p) IP WB Rabbit Log in to see Polyclonal 1
1 ABIN252164 IHC IHC (fro) IHC (p) IP SimWes WB Rabbit Log in to see Polyclonal
1 ABIN187401 IHC (fro) IF IHC (p) IP WB Rat IgG2a Log in to see 9H10
1 ABIN1585714 IP IHC ELISA WB Rabbit C-Term Log in to see Polyclonal
1 ABIN4354644 ELISA IHC IHC (p) IP WB Rabbit C-Term Log in to see Polyclonal
1 ABIN957207 IHC (fro) IHC (p) IP WB Rabbit Log in to see Polyclonal
1 ABIN957208 IHC (fro) IHC (p) IP WB Rabbit Log in to see Polyclonal
1 ABIN352289 IP WB Mouse IgG3 AA 1-55 Log in to see
1 ABIN352284 IHC (p) IP WB Rabbit IgG Log in to see Polyclonal
1 ABIN2362653 IC FACS IP IHC ELISA WB Rat IgG2a AA 55-237 Log in to see 6D767
1 ABIN2362652 IC FACS IP ELISA WB Rat IgG2a AA 55-237 Log in to see 6D766
1 ABIN4354639 IHC IHC (p) IP WB Rabbit Log in to see Polyclonal
1 ABIN187400 IF IP WB Rat IgG2a Log in to see 10G7
1 ABIN199860 ICC FACS IP ELISA WB Rat IgG2a Log in to see
1 ABIN199861 ICC FACS IP IHC ELISA WB Rat IgG2a Log in to see

General

Antigen Second Mitochondria-Derived Activator of Caspase (DIABLO) Antibodies
Reactivity Human, Mouse (Murine), Rat (Rattus)
(185), (70), (56), (4), (4), (3), (3), (3), (3), (2), (1), (1)
Host Mouse
(137), (52), (7), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This DIABLO antibody is un-conjugated
(2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
(183), (73), (66), (50), (30), (19), (8), (8), (7), (4), (4), (3), (2), (2), (1), (1), (1), (1), (1)
Pubmed 9 references available
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Product Details anti-DIABLO Antibody

Target Details DIABLO Application Details Handling ProductDetails: References for anti-DIABLO antibody (ABIN153019) Images
Purification Unpurified
Immunogen Recombinant mature human Smac (lacks amino acids 1-55 present in Smac precursor). [UniProt# Q9NR28]
Clone 78-1-118
Isotype IgG3, IgG1

Target Details DIABLO

Product Details anti-DIABLO Antibody Application Details Handling ProductDetails: References for anti-DIABLO antibody (ABIN153019) Images back to top
Antigen
Alternative Name SMAC/Diablo (DIABLO Antibody Abstract)
Background Gene Symbol: DIABLO
Molecular Weight Theoretical MW: 25 kDa
Gene ID 56616
UniProt Q9NR28
Pathways Apoptosis, Caspase Cascade in Apoptosis, Positive Regulation of Endopeptidase Activity

Application Details

Product Details anti-DIABLO Antibody Target Details DIABLO Handling ProductDetails: References for anti-DIABLO antibody (ABIN153019) Images back to top
Application Notes Western Blot 1:500, Immunocytochemistry/Immunofluorescence, Immunoprecipitation 1:10-1:500This Smac (78-1-118) antibody is useful for Immunoprecipitation and Western Blot, where a band is seen at ~25 kDa. For better results IP prior to Western blot is recommended. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Protocol Immunoprecipitation protocol specific for Smac Antibody Procedure Guide for NB 500-213 Monoclonal anti-Survivin (clone 58.22)Immunoprecipitation Procedure1) Lyse cells plated in a 60mm dish:a) 300 µL CHAPS buffer [50mM Tris-HCl, pH 7.5/50mM NaCl/1mM EDTA/1 % NP-40/0.1 % CHAPS/1mM NaVO4/1mM PMSF]b) rock for 20 minutes at 4C2) Harvest lysate and spin down the insoluble material at 14K rpm. 3) Collect soluble fraction.
4) Pre-clear lysate with 40 µL of 50:50 slurry of Protein A beads, rocking for 1 hour at 4C.
) Spin down beads at 2K rpm, at 4C.
) Collect pre-cleared lysate.
) Incubate lysate with 5-7ug of anti-Survivin overnight, rocking at 4C. 8) Add 50 µL of Protein A 50:50 slurry for 2 hours, rocking at 4C.
) Wash beads with 200 µL of CHAPS buffer, three times.
) Denature immune complex by adding 2x Sample
Restrictions For Research Use only

Handling

Product Details anti-DIABLO Antibody Target Details DIABLO Application Details ProductDetails: References for anti-DIABLO antibody (ABIN153019) Images back to top
Format Liquid
Buffer Ascites
Buffer contains: 0.1 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles
Storage -80 °C,-20 °C
Storage Comment Aliquot and store at -20°C or -80°C. Avoid freeze-thaw cycles.

ProductDetails: References for anti-DIABLO antibody (ABIN153019)

Product Details anti-DIABLO Antibody Target Details DIABLO Application Details Handling Images back to top
Product cited in:

Hwang, Dornbos, Steidemann, Dunivin, Rizzo, LaPres: "Mitochondrial-targeted aryl hydrocarbon receptor and the impact of 2,3,7,8-tetrachlorodibenzo-p-dioxin on cellular respiration and the mitochondrial proteome." in: Toxicology and applied pharmacology, 2016 (Sample species: Mouse (Murine)). Further details: Western Blotting

Sirianni, Jiang, Zeng, Mao, Zhou, Sugarbaker, Zhang, Li, Friedlander, Wang: "N-acetyl-l-tryptophan, but not N-acetyl-d-tryptophan, rescues neuronal cell death in models of amyotrophic lateral sclerosis." in: Journal of neurochemistry, Vol. 134, Issue 5, pp. 956-68, 2015 (Sample species: Mouse (Murine)). Further details: Western Blotting

Zhang, Cook, Kim, Baranov, Jiang, Smith, Cormier, Bennett, Browser, Day, Carlisle, Ferrante, Wang, Friedlander: "Melatonin inhibits the caspase-1/cytochrome c/caspase-3 cell death pathway, inhibits MT1 receptor loss and delays disease progression in a mouse model of amyotrophic lateral sclerosis." in: Neurobiology of disease, Vol. 55, pp. 26-35, 2013 (Sample species: Mouse (Murine)). Further details: Western Blotting

Wang, Sirianni, Pei, Cormier, Smith, Jiang, Zhou, Wang, Zhao, Yano, Kim, Li, Kristal, Ferrante, Friedlander: "The melatonin MT1 receptor axis modulates mutant Huntingtin-mediated toxicity." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 31, Issue 41, pp. 14496-507, 2011

Zhang, Wang, Wang, Narayanan, Stavrovskaya, Kristal, Friedlander: "Nortriptyline protects mitochondria and reduces cerebral ischemia/hypoxia injury." in: Stroke; a journal of cerebral circulation, Vol. 39, Issue 2, pp. 455-62, 2008

Wang, Zhu, Pei, Drozda, Stavrovskaya, Del Signore, Cormier, Shimony, Wang, Ferrante, Kristal, Friedlander: "Inhibitors of cytochrome c release with therapeutic potential for Huntington's disease." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 28, Issue 38, pp. 9473-85, 2008 Further details: Western Blotting

Liu, Deng, Lehal, Kim, Zacksenhaus: "Identification of tumorsphere- and tumor-initiating cells in HER2/Neu-induced mammary tumors." in: Cancer research, Vol. 67, Issue 18, pp. 8671-81, 2007

Wang, Zhu, Drozda, Zhang, Stavrovskaya, Cattaneo, Ferrante, Kristal, Friedlander: "Minocycline inhibits caspase-independent and -dependent mitochondrial cell death pathways in models of Huntington's disease." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 100, Issue 18, pp. 10483-7, 2003 Further details: Immunocytochemistry,Western Blotting,Immunofluorescence

Zhang, Wang, Narayanan, Zhang, Huo, Reed, Friedlander: "Fundamental role of the Rip2/caspase-1 pathway in hypoxia and ischemia-induced neuronal cell death." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 100, Issue 26, pp. 16012-7, 2003 (Sample species: Mouse (Murine)). Further details: Western Blotting

Images

Product Details anti-DIABLO Antibody Target Details DIABLO Application Details Handling ProductDetails: References for anti-DIABLO antibody (ABIN153019) back to top
Supplier Images
Western Blotting (WB) image for anti-Second Mitochondria-Derived Activator of Caspase (DIABLO) antibody (ABIN153019) Western Blot: Smac Antibody (78-1-118) [ABIN153019] - HeLa whole cell extract lysates.