anti-Pig (Porcine) DIABLO antibody for Western Blotting

Recommended DIABLO Antibody (supplied by: Log in to see )

Antigen
Second Mitochondria-Derived Activator of Caspase (DIABLO) Antibodies
  • smac
  • smac3
  • diablo-s
  • 0610041G12Rik
  • 1700006L01Rik
  • AU040403
  • Smac
  • DFNA64
  • SMAC
  • DBO
  • XSmac
  • diablo, IAP-binding mitochondrial protein
  • diablo homolog (Drosophila)
  • diablo
  • Diablo
  • DIABLO
Epitope
C-Term
39
31
13
11
8
6
4
4
4
3
2
2
2
1
1
1
1
Reactivity
Cow (Bovine), Dog (Canine), Horse (Equine), Human, Pig (Porcine)
185
71
57
4
3
3
2
2
2
2
1
1
Host
Rabbit
136
53
7
1
Clonality
Polyclonal
Conjugate
This DIABLO antibody is un-conjugated
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (IHC), Western Blotting (WB)
183
73
65
50
31
20
9
8
7
4
4
3
2
2
1
1
1
1
1
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Catalog No. ABIN2792127
$ 229.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
2.906538 ABIN925989 WB Rabbit C-Term Log in to see Polyclonal
1 ABIN1585714 IP IHC ELISA WB Rabbit C-Term Log in to see Polyclonal

General

Antigen Second Mitochondria-Derived Activator of Caspase (DIABLO) Antibodies
Epitope C-Term
(39), (31), (13), (11), (8), (6), (4), (4), (4), (3), (2), (2), (2), (1), (1), (1), (1)
Reactivity Cow (Bovine), Dog (Canine), Horse (Equine), Human, Pig (Porcine)
(185), (71), (57), (4), (3), (3), (2), (2), (2), (2), (1), (1)
Host Rabbit
(136), (53), (7), (1)
Clonality
Polyclonal
Conjugate This DIABLO antibody is un-conjugated
(2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunohistochemistry (IHC), Western Blotting (WB)
(183), (73), (65), (50), (31), (20), (9), (8), (7), (4), (4), (3), (2), (2), (1), (1), (1), (1), (1)
Pubmed 1 reference available
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Product Details anti-DIABLO Antibody

Target Details DIABLO Application Details Handling References for anti-DIABLO antibody (ABIN2792127) Images
Sequence LVKLQVEEVH QLSRKAETKL AEAQIEELRQ KTQEEGEERA ESEQEAYLRE
Predicted Reactivity Cow: 85%, Dog: 92%, Horse: 85%, Human: 100%, Pig: 85%
Characteristics This is a rabbit polyclonal antibody against DIABLO. It was validated on Western Blot using a cell lysate as a positive control.
Purification Protein A purified
Immunogen The immunogen is a synthetic peptide directed towards the C terminal region of human DIABLO

Target Details DIABLO

Product Details anti-DIABLO Antibody Application Details Handling References for anti-DIABLO antibody (ABIN2792127) Images back to top
Antigen
Alternative Name DIABLO (DIABLO Antibody Abstract)
Background DIABLO is an inhibitor of apoptosis protein (IAP)-binding protein. The encoded mitochondrial protein enters the cytosol when cells undergo apoptosis, and it moderates the caspase inhibition of IAPs. Multiple polyadenylation sites have been found for this gene. Several alternatively spliced transcript variants that encode distinct isoforms have been described for this gene but the validity of some transcripts, and their predicted ORFs, has not been determined conclusively.
Alias Symbols: SMAC, SMAC3, DFNA64, DIABLO-S
Protein Interaction Partner: BIRC7, ARL6IP1, UBC, XIAP, BIRC2, BIRC6, BIRC3, MDM2, GABPA, AP1B1, SSRP1, ABCE1, GMPPB, LIMCH1, BMH1, AREL1, SLC25A47, MAML2, UBL4A, NR4A1, ARNT, MALT1, BIRC8, HTRA2, CDC40, DIABLO, ELAVL1, UBE2K, ZNF746, TOLLIP, UFM1, TRAF2, TRAF1, EEF1A1, Ngfrap1, BIRC
Protein Size: 186
Molecular Weight 21 kDa
Gene ID 56616
NCBI Accession NM_138930, NP_620308
UniProt Q502X2
Research Area Apoptosis/Necrosis, DNA/RNA
Pathways Apoptosis, Caspase Cascade in Apoptosis, Positive Regulation of Endopeptidase Activity

Application Details

Product Details anti-DIABLO Antibody Target Details DIABLO Handling References for anti-DIABLO antibody (ABIN2792127) Images back to top
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 186 AA

Restrictions For Research Use only

Handling

Product Details anti-DIABLO Antibody Target Details DIABLO Application Details References for anti-DIABLO antibody (ABIN2792127) Images back to top
Format Liquid
Concentration Lot specific
Buffer Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-DIABLO antibody (ABIN2792127)

Product Details anti-DIABLO Antibody Target Details DIABLO Application Details Handling Images back to top
Background publications

Samuel, Welsh, Lober, Togo, Zapata, Reed: "Distinct BIR domains of cIAP1 mediate binding to and ubiquitination of tumor necrosis factor receptor-associated factor 2 and second mitochondrial activator of caspases." in: The Journal of biological chemistry, Vol. 281, Issue 2, pp. 1080-90, 2006

Images

Product Details anti-DIABLO Antibody Target Details DIABLO Application Details Handling References for anti-DIABLO antibody (ABIN2792127) back to top
Supplier Images
Western Blotting (WB) image for anti-Second Mitochondria-Derived Activator of Caspase (DIABLO) (C-Term) antibody (ABIN2792127) WB Suggested Anti-DIABLO Antibody Titration: 0.3125ug/ml
ELISA Titer: