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|Antigen||Second Mitochondria-Derived Activator of Caspase (DIABLO) Antibodies|
|Reactivity||Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This DIABLO antibody is un-conjugated Alternatives|
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
|9 references available|
|Supplier||Log in to see|
Product Details anti-DIABLO AntibodyTarget Details DIABLO Application Details Handling References for anti-DIABLO antibody (ABIN153019) Images
|Immunogen||Recombinant mature human Smac (lacks amino acids 1-55 present in Smac precursor). [UniProt# Q9NR28]|
Target Details DIABLOProduct Details anti-DIABLO Antibody Application Details Handling References for anti-DIABLO antibody (ABIN153019) Images back to top
|Alternative Name||SMAC/Diablo (DIABLO Antibody Abstract)|
|Background||Gene Symbol: DIABLO|
|Molecular Weight||Theoretical MW: 25 kDa|
|Pathways||Apoptosis, Caspase Cascade in Apoptosis, Positive Regulation of Endopeptidase Activity|
Application DetailsProduct Details anti-DIABLO Antibody Target Details DIABLO Handling References for anti-DIABLO antibody (ABIN153019) Images back to top
|Application Notes||Western Blot 1:500, Immunocytochemistry/Immunofluorescence, Immunoprecipitation 1:10-1:500This Smac (78-1-118) antibody is useful for Immunoprecipitation and Western Blot, where a band is seen at ~25 kDa. For better results IP prior to Western blot is recommended. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.|
The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.
Immunoprecipitation protocol specific for Smac Antibody Procedure Guide for NB 500-213 Monoclonal anti-Survivin (clone 58.22)Immunoprecipitation Procedure1) Lyse cells plated in a 60mm dish:a) 300 µL CHAPS buffer [50mM Tris-HCl, pH 7.5/50mM NaCl/1mM EDTA/1 % NP-40/0.1 % CHAPS/1mM NaVO4/1mM PMSF]b) rock for 20 minutes at 4C2) Harvest lysate and spin down the insoluble material at 14K rpm. 3) Collect soluble fraction.
4) Pre-clear lysate with 40 µL of 50:50 slurry of Protein A beads, rocking for 1 hour at 4C.
) Spin down beads at 2K rpm, at 4C.
) Collect pre-cleared lysate.
) Incubate lysate with 5-7ug of anti-Survivin overnight, rocking at 4C. 8) Add 50 µL of Protein A 50:50 slurry for 2 hours, rocking at 4C.
) Wash beads with 200 µL of CHAPS buffer, three times.
) Denature immune complex by adding 2x Sample
|Restrictions||For Research Use only|
HandlingProduct Details anti-DIABLO Antibody Target Details DIABLO Application Details References for anti-DIABLO antibody (ABIN153019) Images back to top
Buffer contains: 0.1 % Sodium Azide
|Precaution of Use||This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid freeze-thaw cycles|
|Storage||-80 °C,-20 °C|
|Storage Comment||Aliquot and store at -20°C or -80°C. Avoid freeze-thaw cycles.|
References for anti-DIABLO antibody (ABIN153019)Product Details anti-DIABLO Antibody Target Details DIABLO Application Details Handling Images back to top
|Product cited in:||
Hwang, Dornbos, Steidemann, Dunivin, Rizzo, LaPres: "Mitochondrial-targeted aryl hydrocarbon receptor and the impact of 2,3,7,8-tetrachlorodibenzo-p-dioxin on cellular respiration and the mitochondrial proteome." in: Toxicology and applied pharmacology, 2016 (Sample species: Mouse (Murine)). Further details: Western Blotting
Sirianni, Jiang, Zeng, Mao, Zhou, Sugarbaker, Zhang, Li, Friedlander, Wang: "N-acetyl-l-tryptophan, but not N-acetyl-d-tryptophan, rescues neuronal cell death in models of amyotrophic lateral sclerosis." in: Journal of neurochemistry, Vol. 134, Issue 5, pp. 956-68, 2015 (Sample species: Mouse (Murine)). Further details: Western Blotting
Zhang, Cook, Kim, Baranov, Jiang, Smith, Cormier, Bennett, Browser, Day, Carlisle, Ferrante, Wang, Friedlander: "Melatonin inhibits the caspase-1/cytochrome c/caspase-3 cell death pathway, inhibits MT1 receptor loss and delays disease progression in a mouse model of amyotrophic lateral sclerosis." in: Neurobiology of disease, Vol. 55, pp. 26-35, 2013 (Sample species: Mouse (Murine)). Further details: Western Blotting
Wang, Sirianni, Pei, Cormier, Smith, Jiang, Zhou, Wang, Zhao, Yano, Kim, Li, Kristal, Ferrante, Friedlander: "The melatonin MT1 receptor axis modulates mutant Huntingtin-mediated toxicity." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 31, Issue 41, pp. 14496-507, 2011
Zhang, Wang, Wang, Narayanan, Stavrovskaya, Kristal, Friedlander: "Nortriptyline protects mitochondria and reduces cerebral ischemia/hypoxia injury." in: Stroke; a journal of cerebral circulation, Vol. 39, Issue 2, pp. 455-62, 2008
Wang, Zhu, Pei, Drozda, Stavrovskaya, Del Signore, Cormier, Shimony, Wang, Ferrante, Kristal, Friedlander: "Inhibitors of cytochrome c release with therapeutic potential for Huntington's disease." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 28, Issue 38, pp. 9473-85, 2008 Further details: Western Blotting
Liu, Deng, Lehal, Kim, Zacksenhaus: "Identification of tumorsphere- and tumor-initiating cells in HER2/Neu-induced mammary tumors." in: Cancer research, Vol. 67, Issue 18, pp. 8671-81, 2007
Wang, Zhu, Drozda, Zhang, Stavrovskaya, Cattaneo, Ferrante, Kristal, Friedlander: "Minocycline inhibits caspase-independent and -dependent mitochondrial cell death pathways in models of Huntington's disease." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 100, Issue 18, pp. 10483-7, 2003 Further details: Immunocytochemistry,Western Blotting,Immunofluorescence
Zhang, Wang, Narayanan, Zhang, Huo, Reed, Friedlander: "Fundamental role of the Rip2/caspase-1 pathway in hypoxia and ischemia-induced neuronal cell death." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 100, Issue 26, pp. 16012-7, 2003 (Sample species: Mouse (Murine)). Further details: Western Blotting
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