You are viewing an incomplete version of our website. Please click to reload the website as full version.

Human DIABLO ELISA Kit

Recommended DIABLO Product (supplied by: Log in to see )

Antigen
Second Mitochondria-Derived Activator of Caspase (DIABLO) ELISA Kits
  • 0610041G12Rik
  • 1700006L01Rik
  • AU040403
  • DBO
  • DFNA64
  • diablo-s
  • SMAC
  • Smac
  • smac
  • smac3
  • XSmac
Epitope
AA 56-239
Reactivity
Human
Alternatives
Kits with alternative reactivity to:
9
1
1
1
Methode Type
Sandwich ELISA
Detection Range
31.2-2000 pg/mL
Minimum Detection Limit
31.2 pg/mL
Application
ELISA
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

It's quick and easy to submit your validation proposal. I want to validate this product

Learn more

Available images

Catalog No. ABIN1889324
$ 369.00
Plus shipping costs $45.00
Relevance Score ABIN Method Type Sample Type Detection Range Detection Minimum Supplier References Details
0.30628014 ABIN423865 Tissue Homogenate, Cell Lysate, Biological Fluids 0.15-10 ng/mL 0.15 ng/mL Log in to see
0.30628014 ABIN2708374 Sandwich ELISA Cell Culture Supernatant, Serum, Plasma 31.2-2000 pg/mL 31.2 pg/mL Log in to see
0.0012155735 ABIN813587 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate 23.44-1500 pg/mL 23.44 pg/mL Log in to see
0.0012155735 ABIN625468 Sandwich ELISA Cell Culture Supernatant, Plasma, Serum, Urine 0.1-30 ng/mL 0.1 ng/mL Log in to see
0.0012155735 ABIN2684049 Sandwich ELISA Plasma (heparin), Plasma (EDTA), Cell Culture Supernatant, Serum 31.2-2000 pg/mL 31.2 pg/mL Log in to see
0.0012155735 ABIN2851109 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids 0.156-10 ng/mL 0.156 ng/mL Log in to see
0.0012155735 ABIN2043335 Sandwich ELISA 0.15-10 ng/mL 0.15 ng/mL Log in to see
0.0012155735 ABIN2543821 Sandwich ELISA Tissue Homogenate, Cell Lysate, Biological Fluids 0.156-10 ng/mL 0.156 ng/mL Log in to see
0.0012155735 ABIN2946996 Log in to see

Top referenced DIABLO ELISA Kit for ELISA

Similar DIABLO ELISA Kits

Application / Reactivity Human
ELISA 10 ELISA Kits

General

Antigen Second Mitochondria-Derived Activator of Caspase (DIABLO) ELISA Kits
Epitope AA 56-239
Reactivity Human
Kits with alternative reactivity to:
(9), (1), (1), (1)
Methode Type Sandwich ELISA
Detection Range 31.2-2000 pg/mL
Minimum Detection Limit 31.2 pg/mL
Application ELISA
Pubmed 1 reference available
Supplier Log in to see

Product Details DIABLO ELISA Kit

Target details Application Details Handling References for DIABLO Kit (ABIN1889324) Images
Purpose For quantitative detection of human Diablo in cell culture supernates, serum and plasma(heparin, EDTA).
Brand PicoKine™
Sample Type Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
Detection Method Colorimetric
Specificity Natural and recombinant human Diablo
Cross-Reactivity (Details) There is no detectable cross-reactivity with other relevant proteins.
Sensitivity <10pg/mL
Components
  • 96-well plate precoated with anti- human Diablo antibod - 1
  • Lyophilized recombinant human Diablo standar - 10ng/tubex2
  • Biotinylated anti- human Diablo antibod - 130μl(dilution 1:100)
  • Avidin-Biotin-Peroxidase Complex (ABC - 130μl(dilution 1:100)
  • Sample diluent buffe - 30 ml
  • Antibody diluent buffe - 12ml
  • ABC diluent buffe - 12ml
  • TMB color developing agen - 10ml
  • TMB stop solutio - 10ml
Material not included Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
Immunogen Expression system for standard: E.coli
Immunogen sequence: A56-D239

Target details

Product Details DIABLO ELISA Kit Application Details Handling References for DIABLO Kit (ABIN1889324) Images back to top
Antigen
Alternative Name DIABLO (DIABLO ELISA Kit Abstract)
Background Diablo homolog, mitochondrial, also known as second mitochondria-derived activator of caspases(SMAC), is a protein that in humans is encoded by the DIABLO gene(direct IAP binding protein with low pI). It is mapped to 12q24.31. DIABLO can bind mammalian IAP homolog A(MIHA, or API3) and can also interact with MIHB, MIHC, and OpIAP, the baculoviral IAP. This gene encodes an inhibitor of apoptosis protein(IAP)-binding protein. The encoded mitochondrial protein enters the cytosol when cells undergo apoptosis, and allows activation of caspases by binding to inhibitor of apoptosis proteins. Over expression of the encoded protein sensitizes tumor cells to apoptosis. A mutation in this gene is associated with young-adult onset of nonsyndromic deafness-64.
Gene ID 56616
UniProt Q9NR28
Research Area Apoptosis/Necrosis, DNA/RNA
Pathways Apoptosis, Caspase Cascade in Apoptosis

Application Details

Product Details DIABLO ELISA Kit Target details Handling References for DIABLO Kit (ABIN1889324) Images back to top
Application Notes Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
Plate Pre-coated,Strips (12 x 8)
Protocol human Diablo ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Diablo has been precoated onto 96-well plates. Standards(E.coli, A56-D239) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Diablo is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Diablo amount of sample captured in plate.
Assay Procedure Aliquot 0.1 mL per well of the 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL human Diablo standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human Diablo standard solution and each sample be measured in duplicate.
Assay Precision
  • Sample 1: n=16, Mean(pg/ml): 112, Standard deviation: 6.27, CV(%): 5.6
  • Sample 2: n=16, Mean(pg/ml): 545, Standard deviation: 23.98, CV(%): 4.4
  • Sample 3: n=16, Mean(pg/ml): 1134, Standard deviation: 58.97, CV(%): 5.2,
  • Sample 1: n=24, Mean(pg/ml): 129, Standard deviation: 7.99, CV(%): 6.2
  • Sample 2: n=24, Mean(pg/ml): 577, Standard deviation: 40.97, CV(%): 7.1
  • Sample 3: n=24, Mean(pg/ml): 1133, Standard deviation: 77.04, CV(%): 6.8
Restrictions For Research Use only

Handling

Product Details DIABLO ELISA Kit Target details Application Details References for DIABLO Kit (ABIN1889324) Images back to top
Handling Advice Avoid multiple freeze-thaw cycles
Storage -20 °C,4 °C
Storage Comment Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
Expiry Date 12 months

References for DIABLO Kit (ABIN1889324)

Product Details DIABLO ELISA Kit Target details Application Details Handling Images back to top
Background publications

McNeish, Bell, McKay, Tenev, Marani, Lemoine: "Expression of Smac/DIABLO in ovarian carcinoma cells induces apoptosis via a caspase-9-mediated pathway." in: Experimental cell research, Vol. 286, Issue 2, pp. 186-98, 2003

Images

Product Details DIABLO ELISA Kit Target details Application Details Handling References for DIABLO Kit (ABIN1889324) back to top
Supplier Images
ELISA image for DIABLO ELISA Kit (Second Mitochondria-Derived Activator of Caspase) (ABIN1889324) Human Diablo/SMAC PicoKine ELISA Kit standard curve