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|Application / Reactivity||Human||Mouse (Murine)||Rat (Rattus)||Monkey|
|Western Blotting (WB)||195 Antibodies||67 Antibodies||22 Antibodies||7 Antibodies|
|Proximity Ligation Assay (PLA)||1 Antibodies|
|Immunoprecipitation (IP)||24 Antibodies||7 Antibodies||7 Antibodies||2 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||34 Antibodies||6 Antibodies||3 Antibodies|
|Immunohistochemistry (IHC)||57 Antibodies||39 Antibodies||10 Antibodies||4 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||1 Antibodies||1 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||1 Antibodies||1 Antibodies|
|Antigen||Fas (TNFRSF6)-Associated Via Death Domain (FADD) Antibodies|
|Reactivity||Human, Monkey, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This FADD antibody is un-conjugated Alternatives|
Immunoprecipitation (IP), Western Blotting (WB)
|3 references available|
|Supplier||Log in to see|
Product Details anti-FADD AntibodyTarget Details FADD Application Details Handling References for anti-FADD Antibody (ABIN222894) Images
|Blocking Peptide||Blocking peptide for this product available: ABIN412728|
|Specificity||The antibody detects an ~28 kDa band, corresponding to the expected molecular mass of FADD on immunoblots.|
|Immunogen||Synthetic peptide surrounding amino acid 128 of human FADD|
Target Details FADDProduct Details anti-FADD Antibody Application Details Handling References for anti-FADD Antibody (ABIN222894) Images back to top
|Alternative Name||FADD (FADD Antibody Abstract)|
Fas-associating death domain containing protein (FADD), also known as mediator of receptor induced toxicity (MORT1), is a 23 kDa cytosolic protein with an apparent molecular weight of 28 kDa on SDS-PAGE. The protein is involved in both Fas and TNF-R induced cell death pathway. FADD specifically binds to Fas to initiate the apoptotic signalling cascade. Overexpression of FADD induces apoptosis.
Synonyms: FAS-associated death domain protein, GIG3, MGC8528, MORT1
|Research Area||Cancer, Apoptosis/Necrosis|
|Pathways||Apoptosis, TLR Signaling|
Application DetailsProduct Details anti-FADD Antibody Target Details FADD Handling References for anti-FADD Antibody (ABIN222894) Images back to top
Western blot analysis (0.5-4 μg/mL) and can also be used in Immunoprecipitation.
However, the optimal conditions should be determined individually.
Country of Animal Origin: China
|Restrictions||For Research Use only|
HandlingProduct Details anti-FADD Antibody Target Details FADD Application Details References for anti-FADD Antibody (ABIN222894) Images back to top
|Buffer||100 μg (0.2 mg/mL) affinity purified rabbit anti-FADD polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30 % glycerol, 0.5 % BSA, 0.01 % thimerosal.|
|Precaution of Use||This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
The antibody solution should be gently mixed before use.
Avoid freeze-thaw cycles.
|Storage Comment||For long-term storage, aliquot and freeze at -70 °C.|
|Expiry Date||12 months|
References for anti-FADD Antibody (ABIN222894)Product Details anti-FADD Antibody Target Details FADD Application Details Handling Images back to top
|Product cited in:||
Jin, Ojcius, Sun, Dong, Luo, Mao, Yan: "Leptospira interrogans induces apoptosis in macrophages via caspase-8- and caspase-3-dependent pathways." in: Infection and immunity, Vol. 77, Issue 2, pp. 799-809, 2009
Chen, Texada, Duggan, Liang, Reden, Kooragayala, Langford: "Surface calreticulin mediates muramyl dipeptide-induced apoptosis in RK13 cells." in: The Journal of biological chemistry, Vol. 280, Issue 23, pp. 22425-36, 2005
Piazzolla, Meissl, Kucerova, Rubiolo, Baccarini: "Raf-1 sets the threshold of Fas sensitivity by modulating Rok-alpha signaling." in: The Journal of cell biology, Vol. 171, Issue 6, pp. 1013-22, 2005
ImagesProduct Details anti-FADD Antibody Target Details FADD Application Details Handling References for anti-FADD Antibody (ABIN222894) back to top