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|Application / Reactivity||Human|
|Blocking Antibody (Inhibition)||2 Antibodies|
|Cell-ELISA (cELISA)||1 Antibodies|
|Cellular Assay (CA)||1 Antibodies|
|Dot Blot (DB)||1 Antibodies|
|ELISA (Capture)||1 Antibodies|
|Enzyme Immunoassay (EIA)||11 Antibodies|
|Flow Cytometry (FACS)||120 Antibodies|
|Functional Studies (Func)||23 Antibodies|
|Immunoassay (IA)||1 Antibodies|
|Immunochromatography (IC)||3 Antibodies|
|Immunocytochemistry (ICC)||21 Antibodies|
|Immunofluorescence (IF)||16 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||11 Antibodies|
|Immunohistochemistry (IHC)||40 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||8 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||42 Antibodies|
|Immunoprecipitation (IP)||66 Antibodies|
|Antigen||Fas Ligand (TNF Superfamily, Member 6) (FASL) Antibodies|
|Conjugate||This FASL antibody is conjugated to PE Alternatives|
Flow Cytometry (FACS), Mass Cytometry (CyTOF)
|7 references available|
|Supplier||Log in to see|
Product Details anti-FASL AntibodyTarget Details FASL Application Details Handling References for anti-FASL Antibody (ABIN2663099) Images
|Purification||The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody.|
Target Details FASLProduct Details anti-FASL Antibody Application Details Handling References for anti-FASL Antibody (ABIN2663099) Images back to top
|Alternative Name||CD178 (FASL Antibody Abstract)|
|Background||CD178 is a 38-42 kD type II glycoprotein also known as Fas ligand and CD95L. CD178 belongs to the TNF superfamily and is expressed on activated T lymphocytes, NK cells, monocytes, and granulocytes. CD178 is also expressed on parenchymal cells of the retina and cornea, retinal pigment epithelial cells, and testis. The extracellular region of FasL can be cleaved by matrix metalloproteinases (MMPs) to give rise to a 26 kD soluble protein. CD178 binds to CD95, a member of the TNFR superfamily, to induce apoptosis. CD95/CD95L interactions play an important role in the maintenance of peripheral tolerance and survival.|
|Pathways||Apoptosis, EGFR Signaling Pathway|
Application DetailsProduct Details anti-FASL Antibody Target Details FASL Handling References for anti-FASL Antibody (ABIN2663099) Images back to top
|Application Notes||Optimal working dilution should be determined by the investigator.|
|Restrictions||For Research Use only|
HandlingProduct Details anti-FASL Antibody Target Details FASL Application Details References for anti-FASL Antibody (ABIN2663099) Images back to top
|Buffer||Phosphate-buffered solution, pH 7.2, containing 0.09 % sodium azide and 0.2 % (w/v) BSA .|
|Precaution of Use||This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Protect from prolonged exposure to light. Do not freeze.|
|Storage Comment||The antibody solution should be stored undiluted between 2°C and 8°C.|
References for anti-FASL Antibody (ABIN2663099)Product Details anti-FASL Antibody Target Details FASL Application Details Handling Images back to top
Li, DAlessio, Pober: "Lipopolysaccharide can trigger a cathepsin B-dependent programmed death response in human endothelial cells." in: The American journal of pathology, Vol. 175, Issue 3, pp. 1124-35, 2009 (PubMed).
Strauss, Bergmann, Whiteside: "Human circulating CD4+CD25highFoxp3+ regulatory T cells kill autologous CD8+ but not CD4+ responder cells by Fas-mediated apoptosis." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 182, Issue 3, pp. 1469-80, 2009 (PubMed).
Andreola, Rivoltini, Castelli et al.: "Induction of lymphocyte apoptosis by tumor cell secretion of FasL-bearing microvesicles." in: The Journal of experimental medicine, Vol. 195, Issue 10, pp. 1303-16, 2002 (PubMed).
Herr, Posovsky, Böhler et al.: "mAb33 from transduction laboratories specifically binds human CD95-L." in: Cell death and differentiation, Vol. 7, Issue 1, pp. 129-30, 2000 (PubMed).
Bossi, Griffiths: "Degranulation plays an essential part in regulating cell surface expression of Fas ligand in T cells and natural killer cells." in: Nature medicine, Vol. 5, Issue 1, pp. 90-6, 1999 (PubMed).
Tanaka, Suda, Takahashi et al.: "Expression of the functional soluble form of human fas ligand in activated lymphocytes." in: The EMBO journal, Vol. 14, Issue 6, pp. 1129-35, 1995 (PubMed).
Kayagaki, Kawasaki, Ebata et al.: "Metalloproteinase-mediated release of human Fas ligand." in: The Journal of experimental medicine, Vol. 182, Issue 6, pp. 1777-83, 1996 (PubMed).
ImagesProduct Details anti-FASL Antibody Target Details FASL Application Details Handling References for anti-FASL Antibody (ABIN2663099) back to top