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anti-Human FASL antibody for Enzyme Immunoassay

Recommended FASL Antibody (supplied by: Log in to see )

Antigen
Fas Ligand (TNF Superfamily, Member 6) (FASL) Antibodies
  • ALPS1B
  • APT1LG1
  • Apt1Lg1
  • APTL
  • CD95-L
  • CD95L
  • CD178
  • FAS
  • Fas-L
  • FASL
  • fasl
  • Fasl
  • Faslg
  • FASLG
  • gld
  • TNFRSF6
  • Tnfsf6
  • TNFSF6
  • zgc:162027
Reactivity
Human
274
138
65
3
3
1
1
1
1
1
Host
Mouse
146
126
61
29
12
6
Clonality
Monoclonal
Conjugate
This FASL antibody is un-conjugated
32
26
18
5
3
3
3
1
1
1
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Flow Cytometry (FACS), Immunoprecipitation (IP)
196
162
87
62
57
50
30
29
17
12
11
11
10
6
3
3
2
2
1
1
1
1
1
1
1
Supplier
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Catalog No. ABIN120317
$ 720.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008833851 ABIN120318 EIA FACS IP Mouse IgG1 Log in to see 1OF2 2
0.0008833851 ABIN1105941 EIA Hamster IgG Log in to see 4H9 6
0.0008833851 ABIN120316 EIA Func FACS IP Mouse IgG1 Log in to see 1OF2 2
0.0008833851 ABIN320287 EIA IP Mouse IgG1 Log in to see 14C2 1
0.0008833851 ABIN120319 EIA FACS IP Mouse IgG1 Log in to see 14C2 2
0.0008833851 ABIN120320 EIA FACS IP Mouse IgG1 Log in to see 14C2 1
0.0008833851 ABIN263613 EIA Func FACS Hamster IgG Log in to see 4A5 6
0.0008833851 ABIN187308 EIA Func FACS IHC (fro) IHC (p) IP Mouse IgG1 Log in to see 5G51 1
0.0008833851 ABIN187307 EIA Func FACS IHC (fro) IHC (p) IP Mouse IgG1 Log in to see 2C101
0.0008833851 ABIN2717381 EIA Rabbit IgG Log in to see Polyclonal

Top referenced anti-FASL antibody for Enzyme Immunoassay

Similar anti-FASL Antibodies

Application / Reactivity Human
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Cell-ELISA (cELISA) 1 Antibodies
Cellular Assay (CA) 1 Antibodies
Dot Blot (DB) 1 Antibodies
ELISA 82 Antibodies
ELISA (Capture) 1 Antibodies
ELISpot 1 Antibodies
Enzyme Immunoassay (EIA) 11 Antibodies
Flow Cytometry (FACS) 120 Antibodies
Functional Studies (Func) 23 Antibodies
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Immunofluorescence (IF) 16 Antibodies
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Immunohistochemistry (IHC) 40 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 8 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 42 Antibodies
Immunoprecipitation (IP) 66 Antibodies

General

Antigen Fas Ligand (TNF Superfamily, Member 6) (FASL) Antibodies
Reactivity Human
(274), (138), (65), (3), (3), (1), (1), (1), (1), (1)
Host Mouse
(146), (126), (61), (29), (12), (6)
Clonality (Clone)
Monoclonal   ( )
Conjugate This FASL antibody is un-conjugated
(32), (26), (18), (5), (3), (3), (3), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Flow Cytometry (FACS), Immunoprecipitation (IP)
(196), (162), (87), (62), (57), (50), (30), (29), (17), (12), (11), (11), (10), (6), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Pubmed 2 references available
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Product Details anti-FASL Antibody

Target Details FASL Application Details Handling References for anti-FASL Antibody (ABIN120317) Images
Specificity This antibody recognises the CD178 cell surface antigen. Clone 10F2 is reported to block the activity of Fas ligand. We recommend the use of SM2268LE for such studies.
Purification Affinity Chromatography on Protein G
Immunogen Spleen cells from immunised BALB/c mice were fused with cells of the P3U1 myeloma cell line.
Clone 1OF2
Isotype IgG1

Target Details FASL

Product Details anti-FASL Antibody Application Details Handling References for anti-FASL Antibody (ABIN120317) Images back to top
Antigen
Alternative Name CD178 / Fas Ligand (FASL Antibody Abstract)
Background CD178 is a 40 kDa glycoprotein also known as Fas ligand (CD95L). CD178 is a member of the TNF family, which is expressed by activated T lymphocytes and NK cells. The protein may exist as either a membrane bound or a cleaved soluble form. CD178 plays an important role in T cell development and cytotoxicity. Binding of CD178 to Fas (CD95) results in the induction of apoptosis.Synonyms: APT1LG1, APTL, Apoptosis antigen ligand, CD95L protein, FASL, FASLG, Fas antigen ligand, TNFSF6, Tumor necrosis factor ligand superfamily member 6
Gene ID 356
NCBI Accession NP_000630
UniProt P48023
Research Area CD Antigens, Surface Receptors of Immune Cells
Pathways Apoptosis, EGFR Signaling Pathway

Application Details

Product Details anti-FASL Antibody Target Details FASL Handling References for anti-FASL Antibody (ABIN120317) Images back to top
Application Notes Flow Cytometry: 1/25 - 1/50, Use 10 μL of the suggested working dilution to label 1x10^6 cells in 100 μL. ELISA. Immunoprecipitation.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-FASL Antibody Target Details FASL Application Details References for anti-FASL Antibody (ABIN120317) Images back to top
Concentration 1.0 mg/mL
Buffer PBS, pH 7.4 containing 0.09 % Sodium Azide as preservative
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

References for anti-FASL Antibody (ABIN120317)

Product Details anti-FASL Antibody Target Details FASL Application Details Handling Images back to top
Background publications

Legembre, Moreau, Daburon, Moreau, Taupin: "Potentiation of Fas-mediated apoptosis by an engineered glycosylphosphatidylinositol-linked Fas." in: Cell death and differentiation, Vol. 9, Issue 3, pp. 329-39, 2002

Ferry-Dumazet, Garnier, Mamani-Matsuda, Vercauteren, Belloc, Billiard, Dupouy, Thiolat, Kolb, Marit, Reiffers, Mossalayi: "Resveratrol inhibits the growth and induces the apoptosis of both normal and leukemic hematopoietic cells." in: Carcinogenesis, Vol. 23, Issue 8, pp. 1327-33, 2002

Images

Product Details anti-FASL Antibody Target Details FASL Application Details Handling References for anti-FASL Antibody (ABIN120317) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-FASL antibody (Fas Ligand (TNF Superfamily, Member 6)) (ABIN120317) Staining of CD178 transfected CHO cells with MOUSE ANTI HUMAN CD178 (ABIN120317)