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|Application / Reactivity||Human|
|Blocking Antibody (Inhibition)||2 Antibodies|
|Cell-ELISA (cELISA)||1 Antibodies|
|Cellular Assay (CA)||1 Antibodies|
|Cytometry by Time of Flight (CyTOF)||4 Antibodies|
|Dot Blot (DB)||1 Antibodies|
|ELISA (Capture)||1 Antibodies|
|Enzyme Immunoassay (EIA)||10 Antibodies|
|Flow Cytometry (FACS)||190 Antibodies|
|Functional Studies (Func)||18 Antibodies|
|Immunoassay (IA)||1 Antibodies|
|Immunochromatography (IC)||2 Antibodies|
|Immunocytochemistry (ICC)||30 Antibodies|
|Immunofluorescence (IF)||30 Antibodies|
|Immunofluorescence (fixed cells) (IF/ICC)||7 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||13 Antibodies|
|Immunohistochemistry (IHC)||62 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||26 Antibodies|
|Antigen||Fas Ligand (TNF Superfamily, Member 6) (FASL) Antibodies|
|Conjugate||This FASL antibody is un-conjugated Alternatives|
Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
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Product Details anti-FASL AntibodyTarget Details FASL Application Details Handling Images
|Specificity||This antibody detects Fas Ligand.|
|Cross-Reactivity (Details)||Species reactivity (tested):Human.|
|Characteristics||Synonyms: FASLG, APT1LG1, FASL, TNFSF6, CD95L protein, APTL, Tumor necrosis factor ligandsuperfamily member 6, Fas antigen ligand, Apoptosis antigen ligand|
|Purification||Affiniry Chromatography on Protein G|
|Immunogen||Recombinant Human Fas Ligand.|
Target Details FASLProduct Details anti-FASL Antibody Application Details Handling Images back to top
|Alternative Name||CD178 / Fas Ligand (FASL Antibody Abstract)|
|Background||Fas (APO-1, CD95) is a 45 kDa cell surface protein that mediates apoptosis when crosslinked with agonistic anti-Fas antibodies or Fas ligand (FasL). FasL is a tumor necrosis factor (TNF)-related type II membrane protein and is expressed in various tissue and cells. Both Fas and FasL are thought to play an important role in the apoptosis processes that takes place during T cell development.Synonyms: APT1LG1, APTL, Apoptosis antigen ligand, CD95L protein, FASL, FASLG, Fas antigen ligand, TNFSF6, Tumor necrosis factor ligand superfamily member 6|
|Research Area||CD Antigens, Surface Receptors of Immune Cells|
|Pathways||Apoptosis, EGFR Signaling Pathway, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity|
Application DetailsProduct Details anti-FASL Antibody Target Details FASL Handling Images back to top
Western Blot (1-4 μg/mL): HeLa cell lysate can be used as a Positive Control and a 40 kDaband should be detected. Immunoprecipitation. Immunohistochemistry on Frozen Sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-FASL Antibody Target Details FASL Application Details Images back to top
|Buffer||PBS, pH 7.2, 0.02 % Thimerosal, 50 % Glycerol, 1 % BSA|
|Precaution of Use||This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Storage||-20 °C/-80 °C|
Store the antibody undiluted at -20 °C or for long term storage (in aliquots) at -20 °C -70 °C. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
|Expiry Date||12 months|
ImagesProduct Details anti-FASL Antibody Target Details FASL Application Details Handling back to top