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anti-Mouse (Murine) FASL antibody for Flow Cytometry

Recommended FASL Antibody (supplied by: Log in to see )

Antigen
Fas Ligand (TNF Superfamily, Member 6) (FASL) Antibodies
  • ALPS1B
  • APT1LG1
  • Apt1Lg1
  • APTL
  • CD95-L
  • CD95L
  • CD178
  • FAS
  • Fas-L
  • FASL
  • fasl
  • Fasl
  • Faslg
  • FASLG
  • gld
  • TNFRSF6
  • Tnfsf6
  • TNFSF6
  • zgc:162027
Epitope
AA 132-146
29
25
14
12
11
9
8
6
6
5
3
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
Reactivity
Mouse (Murine)
275
137
65
3
3
1
1
1
1
1
Host
Hamster
146
127
60
29
12
6
Clonality
Monoclonal
Conjugate
This FASL antibody is un-conjugated
32
26
18
5
3
3
3
1
1
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS)
196
162
87
63
57
50
30
29
17
12
11
11
11
6
3
3
2
2
1
1
1
1
1
1
1
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Catalog No. ABIN1105951
$ 434.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.30463836 ABIN1105946 FACS Biotin Hamster IgG Log in to see FLIM58 14
0.30463836 ABIN1105942 FACS Hamster IgG Log in to see FLIM58 14
0.30463836 ABIN1105948 FACS FITC Hamster IgG Log in to see FLIM58 14
0.30463836 ABIN614579 FACS PE Hamster IgG Log in to see MFL4 3
0.30463836 ABIN614573 FACS FITC Hamster IgG Log in to see MFL4 3
0.30463836 ABIN1176980 BR FACS Armenian Hamster IgG3, kappa Log in to see MFL4 11
0.30463836 ABIN2660963 FACS Biotin Hamster IgG Log in to see MFL3 8
0.30463836 ABIN2660964 FACS Biotin Hamster IgG Log in to see MFL4 6
0.30463836 ABIN2663097 FACS PE Hamster IgG Log in to see MFL3 9
0.30463836 ABIN262442 FACS Biotin Armenian Hamster IgG Log in to see MFL4
0.30463836 ABIN2665465 FACS Hamster IgG Log in to see MFL3 9
0.30463836 ABIN2665563 FACS CyTOF IHC Hamster IgG Log in to see MFL3 9
0.30463836 ABIN2665564 FACS IF IHC Hamster IgG Log in to see MFL4 6
0.30463836 ABIN2168297 FACS PE Hamster IgG Log in to see
0.30463836 ABIN2168298 FACS PE Hamster IgG Log in to see
0.30463836 ABIN262444 FACS Biotin Mouse IgG2b kappa Log in to see Kay-10
0.30463836 ABIN262443 FACS IHC (p) IHC Mouse IgG2b kappa Log in to see Kay-10
0.30463836 ABIN447421 FACS FITC Armenian Hamster IgG Log in to see MFL4
0.30463836 ABIN2164963 FACS Hamster IgG Log in to see
0.30463836 ABIN187289 FACS IF WB Rat IgG2a Log in to see H11

Top referenced anti-FASL antibody for Flow Cytometry

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Application / Reactivity Mouse (Murine)
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Functional Studies (Func) 6 Antibodies
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General

Antigen Fas Ligand (TNF Superfamily, Member 6) (FASL) Antibodies
Epitope AA 132-146
(29), (25), (14), (12), (11), (9), (8), (6), (6), (5), (3), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Mouse (Murine)
(275), (137), (65), (3), (3), (1), (1), (1), (1), (1)
Host Hamster
(146), (127), (60), (29), (12), (6)
Clonality (Clone)
Monoclonal   ( )
Conjugate This FASL antibody is un-conjugated
(32), (26), (18), (5), (3), (3), (3), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS)
(196), (162), (87), (63), (57), (50), (30), (29), (17), (12), (11), (11), (11), (6), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Pubmed 5 references available
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Product Details anti-FASL Antibody

Target Details FASL Application Details Handling References for anti-FASL Antibody (ABIN1105951) Images
Sequence PSTPSEKKEP RSVAH
Specificity This antibody reacts with mouse FasL of B6, C3H and MRL mice as well as Balb/c and DBA/1 mice. It also detects gld-type mouse FasL mutant by flow cytometric analysis. We have tested the reactivity on flow cytometry using mouse cell line W40LFL, which is overexpressor of mouse FasL extracellular region (See ref. 1) for more information about this cell line).
Cross-Reactivity (Details) Species reactivity (tested):Mouse
Purification Protein A agarose
Immunogen Synthetic peptide corresponding to the amino acids 132-146 of mouse Fas ligand
Clone FLIM4
Isotype IgG

Target Details FASL

Product Details anti-FASL Antibody Application Details Handling References for anti-FASL Antibody (ABIN1105951) Images back to top
Antigen
Alternative Name CD178 / Fas Ligand (FASL Antibody Abstract)
Background Fas ligand (FasL/CD178/CD95L) is a type II-membrane protein, whose N terminus is in the cytoplasm and its C-terminal region extends into the extracellular space. Its receptor Fas, also termed Apo-1 or CD95, is a cell-surface type I-membrane protein and a member of the tumor necrosis factor (TNF) and nerve growth factor (NGF) receptor family. It is known that engagement of Fas by FasL results in apoptotic cell death. Binding of a trimeric FasL to Fas induces trimerization of Fas, and FADD/MORT1 binds to the trimerized Fas cytoplasmic region through the interaction of the respective death domains. Caspase-8 is then recruited to FADD/MORT1 through binding of each death effector domains (DED), which in turn may induce self-activation of the protease domain and eventually leads cell to apoptosis. FasL is predominantly expressed in activated T lymphocytes and Natural Killer (NK) cells, although it is expressed in the tissues of the immune-privilege sites such as the testis and eye. Furthermore, a variety of cancers such as melanomas were found to express FasL in addition to Fas. On the other hand, Fas is expressed in various tissues with abundant expression in the thymus, liver, heart, and kidney. It is reported that Fas/FasL system is involved in the deletion of activated or autoreactive T and B cells. Mature T cells of normal mice are known to die, after they accomplish their tasks. On the contrary, mature T cells from lpr and gld mice, which are deficient of Fas and FasL respectively, do not die after activation, and activated cells accumulate in the lymph nodes and spleens of these mice. Moreover T cell hybridomas activated in the presence of Fas-neutralizing molecule, do not die. In addition to T cells, the Fas-deficient mice accumulate B cells and have elevated levels of immunoglobulins (Ig) of various classes that include anti-ssDNA and anti-dsDNA antibodies. FasL can be used as an immune-suppressive agent. Rejection of transplants is mediated by activated T cells that may express functional Fas. If a transplanted tissue is engineered to express FasL or is co-transplanted with FasL expressing cells, the transplant may be tolerated. Also Fas/ FasL system has been shown to play a role in human diseases such as fulminant hepatitis, AIDS, cancers and other diseases involving CTL-induced tissue destruction.Synonyms: APT1LG1, APTL, Apoptosis antigen ligand, CD95L protein, FASL, FASLG, Fas antigen ligand, TNFSF6, Tumor necrosis factor ligand superfamily member 6
Gene ID 356
NCBI Accession NP_000630
UniProt P48023
Research Area CD Antigens, Surface Receptors of Immune Cells
Pathways Apoptosis, EGFR Signaling Pathway

Application Details

Product Details anti-FASL Antibody Target Details FASL Handling References for anti-FASL Antibody (ABIN1105951) Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-FASL Antibody Target Details FASL Application Details References for anti-FASL Antibody (ABIN1105951) Images back to top
Concentration 1.0 mg/mL
Buffer PBS containing 50 % glycerol, pH 7.2. No preservative is contained.
Preservative Without preservative
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C
Storage Comment Store (in aliquots) at -20 °C.

References for anti-FASL Antibody (ABIN1105951)

Product Details anti-FASL Antibody Target Details FASL Application Details Handling Images back to top
Background publications

Miwa, Hashimoto, Yatomi, Nakamura, Nagata, Suda: "Therapeutic effect of an anti-Fas ligand mAb on lethal graft-versus-host disease." in: International immunology, Vol. 11, Issue 6, pp. 925-31, 1999

Waring, Müllbacher: "Cell death induced by the Fas/Fas ligand pathway and its role in pathology." in: Immunology and cell biology, Vol. 77, Issue 4, pp. 312-7, 1999

Zeytun, Hassuneh, Nagarkatti, Nagarkatti: "Fas-Fas ligand-based interactions between tumor cells and tumor-specific cytotoxic T lymphocytes: a lethal two-way street." in: Blood, Vol. 90, Issue 5, pp. 1952-9, 1997

Hueber, Zörnig, Lyon, Suda, Nagata, Evan: "Requirement for the CD95 receptor-ligand pathway in c-Myc-induced apoptosis." in: Science (New York, N.Y.), Vol. 278, Issue 5341, pp. 1305-9, 1997

Kayagaki, Yamaguchi, Nagao, Matsuo, Maeda, Okumura, Yagita: "Polymorphism of murine Fas ligand that affects the biological activity." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 94, Issue 8, pp. 3914-9, 1997

Images

Product Details anti-FASL Antibody Target Details FASL Application Details Handling References for anti-FASL Antibody (ABIN1105951) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-FASL antibody (Fas Ligand (TNF Superfamily, Member 6)) (AA 132-146) (ABIN1105951) anti-Fas Ligand (TNF Superfamily, Member 6) (FASL) (AA 132-146) antibody