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anti-Mouse (Murine) FASL antibody for Immunofluorescence

Recommended FASL Antibody (supplied by: Log in to see )

Antigen
Fas Ligand (TNF Superfamily, Member 6) (FASL) Antibodies
  • fasl
  • FASL
  • FASLG
  • FAS
  • TNFRSF6
  • zgc:162027
  • TNFSF6
  • ALPS1B
  • APT1LG1
  • APTL
  • CD178
  • CD95-L
  • CD95L
  • Fas-L
  • Faslg
  • Tnfsf6
  • gld
  • Apt1Lg1
  • Fasl
  • Fas ligand (TNF superfamily, member 6)
  • Fas (TNF receptor superfamily, member 6)
  • fas ligand
  • faslg
  • FAS
  • FASLG
  • fas
  • LOC100344073
  • Fasl
  • Faslg
Reactivity
Human, Mouse (Murine)
375
184
90
22
7
5
4
3
2
1
1
Host
Rat
210
174
59
33
32
8
1
Clonality
Monoclonal
Conjugate
This FASL antibody is un-conjugated
42
32
28
9
9
7
6
6
6
5
5
5
5
5
5
4
3
3
3
2
2
2
2
2
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunofluorescence (IF), Western Blotting (WB)
311
217
105
87
78
63
40
30
28
26
13
12
10
9
5
4
3
3
2
2
1
1
1
1
1
1
1
Supplier
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Available images

Catalog No. ABIN187289
$ 587.40
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN265415 IF IHC (p) WB Rabbit Log in to see Polyclonal
1 ABIN3184595 ELISA IF IHC WB Rabbit IgG Internal Region Log in to see Polyclonal
1 ABIN187305 FACS IF WB Biotin Rat IgG2a Log in to see H11
1 ABIN2665564 FACS IF IHC Hamster IgG Log in to see MFL4 6
1 ABIN2891403 IF IHC WB Rabbit Pro137 Log in to see Polyclonal

Top referenced anti-FASL antibody for Immunofluorescence

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General

Antigen Fas Ligand (TNF Superfamily, Member 6) (FASL) Antibodies
Reactivity Human, Mouse (Murine)
(375), (184), (90), (22), (7), (5), (4), (3), (2), (1), (1)
Host Rat
(210), (174), (59), (33), (32), (8), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This FASL antibody is un-conjugated
(42), (32), (28), (9), (9), (7), (6), (6), (6), (5), (5), (5), (5), (5), (5), (4), (3), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS), Immunofluorescence (IF), Western Blotting (WB)
(311), (217), (105), (87), (78), (63), (40), (30), (28), (26), (13), (12), (10), (9), (5), (4), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1)
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Product Details anti-FASL Antibody

Target Details FASL Application Details Handling Images
Specificity Species Reactivity: Mouse. Others not tested.
Purity > 95 % by SDS-PAGE.
Immunogen Synthetic peptide corresponding to aa 196-220 of the mouse Fas Ligand protein (CD95L, APO-1L, CD178).
Clone H11
Isotype IgG2a

Target Details FASL

Product Details anti-FASL Antibody Application Details Handling Images back to top
Antigen
Alternative Name CD178 / Fas Ligand (FASL Antibody Abstract)
Background Fas Ligand (FasL) is a type II membrane protein and a member of the tumor necrosis factor family. FasL is released by cleavage by metalloproteinases. When interacting with the Fas receptor, the trimeric FasL induces apoptosis. It is responsible for the activation-induced cell death of peripheral T cells and functions as an effector molecule for cytotoxic T cells. FasL is expressed in activated lymphocytes, Sertoli cells, and in certain tumor cells.
Research Area CD Antigens, Surface Receptors of Immune Cells
Pathways Apoptosis, EGFR Signaling Pathway, Production of Molecular Mediator of Immune Response

Application Details

Product Details anti-FASL Antibody Target Details FASL Handling Images back to top
Application Notes Immunocytochemistry: Use anti-Fas Ligand, clone H11 unlabeled (Cat. No. MC-106), biotin-labeled (Cat. No. MC-126), or FITC-labeled (Cat. No. MC-136). Flow Cytometry: Use Anti-Fas Ligand, clone H11 unlabeled (Cat. No. MC-106), biotin-labeled (Cat. No. MC-126), or FITC-labeled (Cat. No. MC-136). [ Note: Activated T cells do not express high levels of surface Fas Ligand.] Western Blot: Excellent for this application. Use 2 µg/mL. Use unlabeled (Cat. No. MC-106) or biotin-labeled (Cat. No. MC-126). The optimal dilution for a specific application should be determined by the researcher. Figure 1. Immunocytochemistry: Detection of FasL in 293T cells transfected with a FasL expression vector. Method: Transfected cells were plated onto polylysine treated glass slides, fixed and permeabilized in methanol at -20 °C for 5 min, then in acetone at -20 °C for 30 sec. After 3 washes in PBS, 0.1% BSA, slides were incubated for 1 hr at RT with 20 µg/mL of biotinylated H11 antibody (MC-126) in PBS, 0.1% BSA. After rinsing in PBS, FITC-conjugated streptavidin was added for 30 min. Slides were washed again in PBS and visualized using a fluorescence microscope. Method: FasL transfected 293T cells (5x105) were incubated on ice for 30 min in 50 µL FACS buffer (PBS, 5% fetal calf serum, 0.02% sodium azide) containing 1 µg of FITC-labeled H11 antibody (MC-136). After washing in FACS buffer, cells were analyzed by flow cytometry. Do not exclude pre-apoptotic cells during data acquisition.
Restrictions For Research Use only

Handling

Product Details anti-FASL Antibody Target Details FASL Application Details Images back to top
Format Liquid
Buffer 100 µL of 1 mg/mL antibody in PBS with 0.02% sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C

Images

Product Details anti-FASL Antibody Target Details FASL Application Details Handling back to top
Supplier Images
Flow Cytometry (FACS) image for anti-FASL antibody (Fas Ligand (TNF Superfamily, Member 6)) (ABIN187289) anti-Fas Ligand (TNF Superfamily, Member 6) (FASL) antibody
Immunofluorescence (IF) image for anti-FASL antibody (Fas Ligand (TNF Superfamily, Member 6)) (ABIN187289) anti-Fas Ligand (TNF Superfamily, Member 6) (FASL) antibody (Image 2)
Western Blotting (WB) image for anti-FASL antibody (Fas Ligand (TNF Superfamily, Member 6)) (ABIN187289) anti-Fas Ligand (TNF Superfamily, Member 6) (FASL) antibody (Image 3)