You are viewing an incomplete version of our website. Please click to reload the website as full version.

anti-Mouse (Murine) FASL antibody for Immunofluorescence

Recommended FASL Antibody (supplied by: Log in to see )

Antigen
Fas Ligand (TNF Superfamily, Member 6) (FASL) Antibodies
  • ALPS1B
  • APT1LG1
  • Apt1Lg1
  • APTL
  • CD95-L
  • CD95L
  • CD178
  • FAS
  • Fas-L
  • FASL
  • fasl
  • Fasl
  • Faslg
  • FASLG
  • gld
  • TNFRSF6
  • Tnfsf6
  • TNFSF6
  • zgc:162027
Reactivity
Human, Mouse (Murine)
274
137
65
3
3
1
1
1
1
1
Host
Rat
146
127
61
28
12
6
Clonality
Monoclonal
Conjugate
This FASL antibody is un-conjugated
32
26
18
5
3
3
3
1
1
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunofluorescence (IF), Western Blotting (WB)
196
161
87
63
57
50
30
29
16
12
11
11
11
6
3
3
2
2
1
1
1
1
1
1
1
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

It's quick and easy to submit your validation proposal. I want to validate this product

Learn more

Available images

Catalog No. ABIN187289
$ 587.40
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0010843406 ABIN265415 IF IHC (p) WB Rabbit Log in to see Polyclonal
0.0010843406 ABIN187305 FACS IF WB Biotin Rat IgG2a Log in to see H11
0.0010843406 ABIN2665564 FACS IF IHC Hamster IgG Log in to see MFL4 6
0.0010843406 ABIN3184595 ELISA IF IHC WB Rabbit IgG Internal Region Log in to see Polyclonal

Top referenced anti-FASL antibody for Immunofluorescence

Similar anti-FASL Antibodies

Application / Reactivity Mouse (Murine) Human
Blocking Reagent (BR) 3 Antibodies
ELISA 8 Antibodies
Flow Cytometry (FACS) 90 Antibodies
Functional Studies (Func) 6 Antibodies
Immunochromatography (IC) 3 Antibodies
Immunocytochemistry (ICC) 8 Antibodies
Immunofluorescence (IF) 5 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies
Immunohistochemistry (IHC) 27 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 3 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 17 Antibodies
Immunoprecipitation (IP) 2 Antibodies
Mass Cytometry (CyTOF) 1 Antibodies
Neutralization (Neut) 3 Antibodies
Western Blotting (WB) 45 Antibodies
Blocking Antibody (Inhibition) 2 Antibodies
Cell-ELISA (cELISA) 1 Antibodies
Cellular Assay (CA) 1 Antibodies
Dot Blot (DB) 1 Antibodies

General

Antigen Fas Ligand (TNF Superfamily, Member 6) (FASL) Antibodies
Reactivity Human, Mouse (Murine)
(274), (137), (65), (3), (3), (1), (1), (1), (1), (1)
Host Rat
(146), (127), (61), (28), (12), (6)
Clonality (Clone)
Monoclonal   ( )
Conjugate This FASL antibody is un-conjugated
(32), (26), (18), (5), (3), (3), (3), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS), Immunofluorescence (IF), Western Blotting (WB)
(196), (161), (87), (63), (57), (50), (30), (29), (16), (12), (11), (11), (11), (6), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Supplier Log in to see

Product Details anti-FASL Antibody

Target Details FASL Application Details Handling Images
Specificity Species Reactivity: Mouse. Others not tested.
Purity > 95 % by SDS-PAGE.
Immunogen Synthetic peptide corresponding to aa 196-220 of the mouse Fas Ligand protein (CD95L, APO-1L, CD178).
Clone H11
Isotype IgG2a

Target Details FASL

Product Details anti-FASL Antibody Application Details Handling Images back to top
Antigen
Alternative Name CD178 / Fas Ligand (FASL Antibody Abstract)
Background Fas Ligand (FasL) is a type II membrane protein and a member of the tumor necrosis factor family. FasL is released by cleavage by metalloproteinases. When interacting with the Fas receptor, the trimeric FasL induces apoptosis. It is responsible for the activation-induced cell death of peripheral T cells and functions as an effector molecule for cytotoxic T cells. FasL is expressed in activated lymphocytes, Sertoli cells, and in certain tumor cells.
Research Area CD Antigens, Surface Receptors of Immune Cells
Pathways Apoptosis, EGFR Signaling Pathway

Application Details

Product Details anti-FASL Antibody Target Details FASL Handling Images back to top
Application Notes Immunocytochemistry: Use anti-Fas Ligand, clone H11 unlabeled (Cat. No. MC-106), biotin-labeled (Cat. No. MC-126), or FITC-labeled (Cat. No. MC-136). Flow Cytometry: Use Anti-Fas Ligand, clone H11 unlabeled (Cat. No. MC-106), biotin-labeled (Cat. No. MC-126), or FITC-labeled (Cat. No. MC-136). [ Note: Activated T cells do not express high levels of surface Fas Ligand.] Western Blot: Excellent for this application. Use 2 µg/mL. Use unlabeled (Cat. No. MC-106) or biotin-labeled (Cat. No. MC-126). The optimal dilution for a specific application should be determined by the researcher. Figure 1. Immunocytochemistry: Detection of FasL in 293T cells transfected with a FasL expression vector. Method: Transfected cells were plated onto polylysine treated glass slides, fixed and permeabilized in methanol at -20 °C for 5 min, then in acetone at -20 °C for 30 sec. After 3 washes in PBS, 0.1% BSA, slides were incubated for 1 hr at RT with 20 µg/mL of biotinylated H11 antibody (MC-126) in PBS, 0.1% BSA. After rinsing in PBS, FITC-conjugated streptavidin was added for 30 min. Slides were washed again in PBS and visualized using a fluorescence microscope. Method: FasL transfected 293T cells (5x105) were incubated on ice for 30 min in 50 µL FACS buffer (PBS, 5% fetal calf serum, 0.02% sodium azide) containing 1 µg of FITC-labeled H11 antibody (MC-136). After washing in FACS buffer, cells were analyzed by flow cytometry. Do not exclude pre-apoptotic cells during data acquisition.
Restrictions For Research Use only

Handling

Product Details anti-FASL Antibody Target Details FASL Application Details Images back to top
Format Liquid
Buffer 100 µL of 1 mg/mL antibody in PBS with 0.02% sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C

Images

Product Details anti-FASL Antibody Target Details FASL Application Details Handling back to top
Supplier Images
Flow Cytometry (FACS) image for anti-FASL antibody (Fas Ligand (TNF Superfamily, Member 6)) (ABIN187289) anti-Fas Ligand (TNF Superfamily, Member 6) (FASL) antibody
Immunofluorescence (IF) image for anti-FASL antibody (Fas Ligand (TNF Superfamily, Member 6)) (ABIN187289) anti-Fas Ligand (TNF Superfamily, Member 6) (FASL) antibody (Image 2)
Western Blotting (WB) image for anti-FASL antibody (Fas Ligand (TNF Superfamily, Member 6)) (ABIN187289) anti-Fas Ligand (TNF Superfamily, Member 6) (FASL) antibody (Image 3)