anti-Mouse (Murine) FASL antibody for Immunohistochemistry (Frozen Sections)

Recommended FASL Antibody (supplied by: Log in to see )

Antigen
Fas Ligand (TNF Superfamily, Member 6) (FASL) Antibodies
  • fasl
  • FASL
  • FASLG
  • FAS
  • TNFRSF6
  • zgc:162027
  • TNFSF6
  • ALPS1B
  • APT1LG1
  • APTL
  • CD178
  • CD95-L
  • CD95L
  • Fas-L
  • Faslg
  • Tnfsf6
  • gld
  • Apt1Lg1
  • Fasl
  • Fas ligand (TNF superfamily, member 6)
  • Fas (TNF receptor superfamily, member 6)
  • fas ligand
  • faslg
  • FAS
  • FASLG
  • fas
  • LOC100344073
  • Fasl
  • Faslg
Epitope
AA 196-220
26
19
17
16
12
9
8
7
7
6
5
5
5
4
3
3
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
Reactivity
Mouse (Murine)
404
171
89
36
7
5
5
3
3
2
1
1
Host
Rat
240
175
49
32
28
8
1
Clonality
Monoclonal
Conjugate
This FASL antibody is un-conjugated
43
30
26
11
11
8
8
8
8
7
7
7
7
7
6
5
5
4
3
2
2
2
2
2
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
296
244
111
95
78
75
38
34
28
27
13
12
10
10
5
4
3
3
2
2
1
1
1
1
1
1
1
Supplier
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Catalog No. ABIN4310543
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN187288 IHC (fro) IHC (p) Rat IgM Log in to see A11

Top referenced anti-FASL antibody for Immunohistochemistry (Frozen Sections)

Similar anti-FASL Antibodies

Application / Reactivity Mouse (Murine)
Blocking Reagent (BR) 3 Antibodies
ELISA 10 Antibodies
Flow Cytometry (FACS) 103 Antibodies
Functional Studies (Func) 9 Antibodies
Immunochromatography (IC) 3 Antibodies
Immunocytochemistry (ICC) 9 Antibodies
Immunofluorescence (IF) 6 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 1 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 13 Antibodies
Immunohistochemistry (IHC) 28 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 2 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 27 Antibodies
Immunoprecipitation (IP) 6 Antibodies
Mass Cytometry (CyTOF) 1 Antibodies
Neutralization (Neut) 4 Antibodies
Western Blotting (WB) 58 Antibodies

General

Antigen Fas Ligand (TNF Superfamily, Member 6) (FASL) Antibodies
Epitope AA 196-220
(26), (19), (17), (16), (12), (9), (8), (7), (7), (6), (5), (5), (5), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Mouse (Murine)
(404), (171), (89), (36), (7), (5), (5), (3), (3), (2), (1), (1)
Host Rat
(240), (175), (49), (32), (28), (8), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This FASL antibody is un-conjugated
(43), (30), (26), (11), (11), (8), (8), (8), (8), (7), (7), (7), (7), (7), (6), (5), (5), (4), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(296), (244), (111), (95), (78), (75), (38), (34), (28), (27), (13), (12), (10), (10), (5), (4), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Pubmed 10 references available
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Product Details anti-FASL Antibody

Target Details FASL Application Details Handling ProductDetails: References for anti-FASL antibody (ABIN4310543) Images
Purification Protein G purified
Immunogen Synthetic peptide corresponding to aa 196-220 of mouse FasL (CD95L, APO-1L, CD178).
Clone A11
Isotype IgM

Target Details FASL

Product Details anti-FASL Antibody Application Details Handling ProductDetails: References for anti-FASL antibody (ABIN4310543) Images back to top
Antigen
Alternative Name Fas Ligand/TNFSF6 (FASL Antibody Abstract)
Background Gene Symbol: FASLG
Gene ID 356
Pathways Apoptosis, EGFR Signaling Pathway, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity

Application Details

Product Details anti-FASL Antibody Target Details FASL Handling ProductDetails: References for anti-FASL antibody (ABIN4310543) Images back to top
Application Notes Flow Cytometry 1:10-1:1000, Immunohistochemistry 1:10-1:500, Immunohistochemistry-Paraffin 1:10-1:500, Immunohistochemistry-Frozen 1:10-1:500Immunohistochemical localization of FasL in adult mouse testis using the A11 antibody. Method: Cryostat sections (6-8um) were air dried, fixed in 4 % paraformaldehyde in PBS and used immediately. Sections were incubated for 1 hr at room temperature (RT) with 20 μg/mL of A11 antibody in PBS, 0.2 % BSA, 0.05 % Tween 20. After rinsing in PBS, a FITC-conjugated anti-rat IgM was added for 30 min., slides washed again in PBS and visualized using a fluorescence microscope. Paraffin sections: Dewax in xylene (2x10 min), EtOH 100 % (2x10 min), block endogeneous peroxidase (0.6 % H2O2 in Methanol 10 min), rehydrate through graded EtOH (100 %, 95 %, 70 %), rinse in H20, unmask epitopes by heating slides in citrate buffer (0.01M, pH 6.0) 2x5 min in microwave oven (650-750W), cool down to RT 20 min, wash 1x in PBS, incubate with A11 20 μg/mL antibody, wash in PBS, incubate in anti-rat IgM HRP (ALX-211-055), wash in PBS, develop in appropriate substrate solution (DAB or AEC).
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only

Handling

Product Details anti-FASL Antibody Target Details FASL Application Details ProductDetails: References for anti-FASL antibody (ABIN4310543) Images back to top
Format Liquid
Concentration 1.0 mg/mL
Buffer PBS
Buffer contains: 0.02 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store at 4°C. Do not freeze.

ProductDetails: References for anti-FASL antibody (ABIN4310543)

Product Details anti-FASL Antibody Target Details FASL Application Details Handling Images back to top
Product cited in:

Viard-Leveugle, Bullani, Meda, Micheau, Limat, Saurat, Tschopp, French: "Intracellular localization of keratinocyte Fas ligand explains lack of cytolytic activity under physiological conditions." in: The Journal of biological chemistry, Vol. 278, Issue 18, pp. 16183-8, 2003

Sata, Luo, Walsh: "Fas ligand overexpression on allograft endothelium inhibits inflammatory cell infiltration and transplant-associated intimal hyperplasia." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 166, Issue 11, pp. 6964-71, 2001

Refaeli, Van Parijs, London, Tschopp, Abbas: "Biochemical mechanisms of IL-2-regulated Fas-mediated T cell apoptosis." in: Immunity, Vol. 8, Issue 5, pp. 615-23, 1998

Li, Rosen, Ronen, Behrens, Krammer, Clark, Berke: "The regulation of CD95 ligand expression and function in CTL." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 161, Issue 8, pp. 3943-9, 1998

Saas, Walker, Hahne, Quiquerez, Schnuriger, Perrin, French, Van Meir, de Tribolet, Tschopp, Dietrich: "Fas ligand expression by astrocytoma in vivo: maintaining immune privilege in the brain?" in: The Journal of clinical investigation, Vol. 99, Issue 6, pp. 1173-8, 1997

Hahne, Peitsch, Irmler, Schröter, Lowin, Rousseau, Bron, Renno, French, Tschopp: "Characterization of the non-functional Fas ligand of gld mice." in: International immunology, Vol. 7, Issue 9, pp. 1381-6, 1996

French, Hahne, Viard, Radlgruber, Zanone, Becker, Müller, Tschopp: "Fas and Fas ligand in embryos and adult mice: ligand expression in several immune-privileged tissues and coexpression in adult tissues characterized by apoptotic cell turnover." in: The Journal of cell biology, Vol. 133, Issue 2, pp. 335-43, 1996

Renno, Hahne, Tschopp, MacDonald: "Peripheral T cells undergoing superantigen-induced apoptosis in vivo express B220 and upregulate Fas and Fas ligand." in: The Journal of experimental medicine, Vol. 183, Issue 2, pp. 431-7, 1996

Hahne, Rimoldi, Schröter, Romero, Schreier, French, Schneider, Bornand, Fontana, Lienard, Cerottini, Tschopp: "Melanoma cell expression of Fas(Apo-1/CD95) ligand: implications for tumor immune escape." in: Science (New York, N.Y.), Vol. 274, Issue 5291, pp. 1363-6, 1996

Knowles, Moncada: "Nitric oxide synthases in mammals." in: The Biochemical journal, Vol. 298 ( Pt 2), pp. 249-58, 1994

Images

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Supplier Images
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-FASL antibody (Fas Ligand (TNF Superfamily, Member 6)) (AA 196-220) (ABIN4310543) Immunohistochemistry-Paraffin: Fas Ligand Antibody (A11) [NBP1-97519] - Immunohistoch...