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anti-Mouse (Murine) FASL antibody for Immunohistochemistry (Frozen Sections)

Recommended FASL Antibody (supplied by: Log in to see )

Antigen
Fas Ligand (TNF Superfamily, Member 6) (FASL) Antibodies
  • ALPS1B
  • APT1LG1
  • Apt1Lg1
  • APTL
  • CD95-L
  • CD95L
  • CD178
  • FAS
  • Fas-L
  • FASL
  • fasl
  • Fasl
  • Faslg
  • FASLG
  • gld
  • TNFRSF6
  • Tnfsf6
  • TNFSF6
  • zgc:162027
Reactivity
Mouse (Murine)
275
137
65
3
3
1
1
1
1
1
Host
Rat
146
127
61
28
12
6
Clonality
Monoclonal
Conjugate
This FASL antibody is un-conjugated
32
26
18
5
3
3
3
1
1
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
197
162
87
63
57
49
30
29
17
11
11
11
11
6
3
3
2
2
1
1
1
1
1
1
1
Supplier
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Catalog No. ABIN187288
$ 569.80
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.000884117 ABIN1867872 ICC IHC (fro) IHC (p) ELISA WB Rabbit IgG AA 132-279 Log in to see Polyclonal
0.000884117 ABIN184442 IHC (fro) WB Rabbit Log in to see Polyclonal

Similar anti-FASL Antibodies

Application / Reactivity Mouse (Murine)
Blocking Reagent (BR) 3 Antibodies
ELISA 8 Antibodies
Flow Cytometry (FACS) 90 Antibodies
Functional Studies (Func) 6 Antibodies
Immunochromatography (IC) 3 Antibodies
Immunocytochemistry (ICC) 8 Antibodies
Immunofluorescence (IF) 5 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies
Immunohistochemistry (IHC) 27 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 3 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 17 Antibodies
Immunoprecipitation (IP) 2 Antibodies
Mass Cytometry (CyTOF) 1 Antibodies
Neutralization (Neut) 3 Antibodies
Western Blotting (WB) 45 Antibodies

General

Antigen Fas Ligand (TNF Superfamily, Member 6) (FASL) Antibodies
Reactivity Mouse (Murine)
(275), (137), (65), (3), (3), (1), (1), (1), (1), (1)
Host Rat
(146), (127), (61), (28), (12), (6)
Clonality (Clone)
Monoclonal   ( )
Conjugate This FASL antibody is un-conjugated
(32), (26), (18), (5), (3), (3), (3), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(197), (162), (87), (63), (57), (49), (30), (29), (17), (11), (11), (11), (11), (6), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1)
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Product Details anti-FASL Antibody

Target Details FASL Application Details Handling Images
Specificity Fas Ligand (FasL) is a type II membrane protein and a member of the tumor necrosis factor family. When interacting with the Fas receptor, the trimeric FasL induces apoptosis. It is responsible for the activation-induced apoptosis of peripheral T cells and functions as an effector molecule for cytotoxic T cells. FasL is expressed in activated lymphocytes, Sertoli cells, and some tumor cells. Species Reactivity: Mouse. Others not tested.
Immunogen Synthetic peptide corresponding to aa 196-220 of the mouse Fas Ligand protein
Clone A11
Isotype IgM

Target Details FASL

Product Details anti-FASL Antibody Application Details Handling Images back to top
Antigen
Alternative Name CD178 / Fas Ligand (FASL Antibody Abstract)
Research Area CD Antigens, Surface Receptors of Immune Cells
Pathways Apoptosis, EGFR Signaling Pathway

Application Details

Product Details anti-FASL Antibody Target Details FASL Handling Images back to top
Application Notes Flow cytometry Immunohistochemistry: Excellent for this application. Frozen Sections- Use 20 µg/mL, incubate 1 hr at room temp. Method: Cryostat sections (6-8 µm) were air dried, fixed in 4% paraformaldehyde in PBS and used immediately. Sections were incubated for 1 hr at RT with 20 µg/mL of A11 antibody in PBS, 0.2% BSA, 0.05% Tween 20. After rinsing in PBS, a FITC-conjugated anti-rat IgM was added for 30 min, slides washed again in PBS and visualized using a fluorescence microscope. Paraffin-Embedded Sections- Dewax in xylene (2 x 10 min), EtOH 100% (2 x 10 min), block endogenous peroxidase (0.6% H2O2 in Methanol 10 min), rehydrate through graded EtOH (100%, 95%, 70%), rinse in H2O, unmask epitopes by heating slides in citrate buffer (0.01 M, pH 6.0) 2 x 5 min in microwave oven (650-750W), cool down to RT 20 min, wash 1x in PBS, incubate with A11 20 µg/mL antibody, wash in PBS, incubate in anti-rat IgM HRP, wash in PBS, develop in appropriate substrate solution (DAB or AEC). The optimal dilution for a specific application should be determined by the researcher.
Restrictions For Research Use only

Handling

Product Details anti-FASL Antibody Target Details FASL Application Details Images back to top
Format Liquid
Buffer 100 µL of 1 mg/mL monoclonal antibody in PBS with 0.02% sodium azide. Purity 95% by SDS-PAGE.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C