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|Application / Reactivity||Rat (Rattus)||Mouse (Murine)|
|Blocking Reagent (BR)||2 Antibodies||3 Antibodies|
|ELISA||8 Antibodies||10 Antibodies|
|Flow Cytometry (FACS)||38 Antibodies||116 Antibodies|
|Functional Studies (Func)||4 Antibodies||9 Antibodies|
|Immunocytochemistry (ICC)||6 Antibodies||9 Antibodies|
|Immunofluorescence (IF)||1 Antibodies||6 Antibodies|
|Immunofluorescence (fixed cells) (IF/ICC)||1 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||13 Antibodies|
|Immunohistochemistry (IHC)||14 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||23 Antibodies|
|Immunoprecipitation (IP)||5 Antibodies|
|Western Blotting (WB)||44 Antibodies|
|Immunochromatography (IC)||3 Antibodies|
|Antigen||Fas Ligand (TNF Superfamily, Member 6) (FASL) Antibodies|
|Epitope||AA 128-279 Alternatives|
|Reactivity||Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This FASL antibody is un-conjugated Alternatives|
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
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Product Details anti-FASL AntibodyTarget Details FASL Application Details Handling Images
|Purpose||Rabbit IgG polyclonal antibody for Tumor necrosis factor ligand superfamily member 6(FASLG) detection. Tested with WB, IHC-P in Mouse,Rat.|
|Cross-Reactivity (Details)||No cross reactivity with other proteins.|
Rabbit IgG polyclonal antibody for Tumor necrosis factor ligand superfamily member 6(FASLG) detection. Tested with WB, IHC-P in Mouse,Rat.
Gene Name: Fas ligand (TNF superfamily, member 6)
Protein Name: Tumor necrosis factor ligand superfamily member 6
|Purification||Immunogen affinity purified.|
|Immunogen||E.coli-derived mouse Fas Ligand recombinant protein (Position: Q128-L279). Mouse Fas Ligand shares 82% and 93% amino acid (aa) sequences identity with human and rat, respectively.|
Target Details FASLProduct Details anti-FASL Antibody Application Details Handling Images back to top
|Alternative Name||FASLG (FASL Antibody Abstract)|
Fas ligand (FasL or CD95L) is a type-II transmembrane protein that belongs to the tumor necrosis factor (TNF) family. Fas ligand/receptor interactions play an important role in the regulation of the immune system and the progression of cancer. It is mapped to 1q24.3. FASL may contribute to the immune privilege of tumors. Pharmacologic products that render infiltrating T cells insensitive to FASL-induced killing may break the immunologic unresponsiveness to melanoma and provide a complementary approach in the therapy of malignant melanoma. FASL-mediated apoptosis is important for skin homeostasis, the dysregulation of FAS-FASL interactions may be central to the development of skin cancer. Polymorphisms affecting either the expression or biologic activities of FAS or FASL may contribute to the genetic risk of developing T-cell lymphoblastic lymphomas.
Synonyms: ALPS1B antibody|APO1L antibody|Apoptosis (APO 1) antigen ligand 1 antibody|Apoptosis antigen ligand 1 antibody|Apoptosis antigen ligand antibody|APT1LG1 antibody|APTL antibody|CD178 antibody|CD178 antigen antibody|CD95 ligand antibody|CD95-L antibody|CD95L antibody|CD95L protein antibody|Fas antigen ligand antibody|Fas L antibody|Fas ligand (TNF superfamily member 6) antibody|Fas ligand antibody|FASL antibody|Fasl Fas ligand (TNF superfamily member 6) antibody|FASLG antibody|Generalized lymphoproliferative disease antibody|Gld antibody|soluble form antibody|TNF superfamily member 6 antibody|TNFL6_HUMAN antibody|TNFSF6 antibody|Tumor necrosis factor (ligand) superfamily member 6 antibody|Tumor necrosis factor ligand superfamily member 6 antibody
|Pathways||Apoptosis, EGFR Signaling Pathway, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity|
Application DetailsProduct Details anti-FASL Antibody Target Details FASL Handling Images back to top
WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Rat, Predicted Species: Mouse, The detection limit for Fas Ligand is approximately 0.25 ng/lane under reducing conditions.
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Mouse, Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.
Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).
|Restrictions||For Research Use only|
HandlingProduct Details anti-FASL Antibody Target Details FASL Application Details Images back to top
|Reconstitution||Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.|
|Buffer||Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.|
|Precaution of Use||This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.
ImagesProduct Details anti-FASL Antibody Target Details FASL Application Details Handling back to top