You are viewing an incomplete version of our website. Please click to reload the website as full version.

anti-Rat (Rattus) FASL antibody for Blocking Reagent

Recommended FASL Antibody (supplied by: Log in to see )

Antigen
Fas Ligand (TNF Superfamily, Member 6) (FASL) Antibodies
  • ALPS1B
  • APT1LG1
  • Apt1Lg1
  • APTL
  • CD95-L
  • CD95L
  • CD178
  • FAS
  • Fas-L
  • FASL
  • fasl
  • Fasl
  • Faslg
  • FASLG
  • gld
  • TNFRSF6
  • Tnfsf6
  • TNFSF6
  • zgc:162027
Reactivity
Mouse (Murine), Rat (Rattus)
275
137
64
3
3
1
1
1
1
1
Host
Armenian Hamster
146
127
61
29
11
6
Clonality
Monoclonal
Conjugate
This FASL antibody is un-conjugated
32
26
18
5
3
3
3
1
1
1
1
1
1
1
1
1
1
1
Application
Blocking Reagent (BR), Flow Cytometry (FACS)
196
162
87
63
57
50
30
29
17
12
11
11
11
6
3
2
2
2
1
1
1
1
1
1
1
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

It's quick and easy to submit your validation proposal. I want to validate this product

Learn more

Available images

Catalog No. ABIN1176980
Contact our Customer Service for availability and price in your country.
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008723726 ABIN2689023 BR FACS Armenian Hamster IgG3, kappa Log in to see MFL4 11

Top referenced anti-FASL antibody for Blocking Reagent

Similar anti-FASL Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine)
Blocking Reagent (BR) 2 Antibodies 3 Antibodies
ELISA 5 Antibodies 8 Antibodies
Flow Cytometry (FACS) 16 Antibodies 90 Antibodies
Functional Studies (Func) 2 Antibodies 6 Antibodies
Immunocytochemistry (ICC) 2 Antibodies 8 Antibodies
Immunofluorescence (IF) 1 Antibodies 5 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies 11 Antibodies
Immunohistochemistry (IHC) 10 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 2 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 16 Antibodies
Immunoprecipitation (IP) 2 Antibodies
Western Blotting (WB) 32 Antibodies
Immunochromatography (IC) 3 Antibodies

General

Antigen Fas Ligand (TNF Superfamily, Member 6) (FASL) Antibodies
Reactivity Mouse (Murine), Rat (Rattus)
(275), (137), (64), (3), (3), (1), (1), (1), (1), (1)
Host Armenian Hamster
(146), (127), (61), (29), (11), (6)
Clonality (Clone)
Monoclonal   ( )
Conjugate This FASL antibody is un-conjugated
(32), (26), (18), (5), (3), (3), (3), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Blocking Reagent (BR), Flow Cytometry (FACS)
(196), (162), (87), (63), (57), (50), (30), (29), (17), (12), (11), (11), (11), (6), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Pubmed 11 references available
Supplier Log in to see

Product Details anti-FASL Antibody

Target Details FASL Application Details Handling References for anti-FASL Antibody (ABIN1176980) Images
Brand BD Pharmingen™
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Sterility 0.2 μm filtered
Endotoxin Level Endotoxin level is ≤ 0.01 EU/μg (≤ 0.001 ng/μg) of protein as determined by the LAL assay.
Immunogen Mouse FasL transfected cells
Clone MFL4
Isotype IgG3, kappa

Target Details FASL

Product Details anti-FASL Antibody Application Details Handling References for anti-FASL Antibody (ABIN1176980) Images back to top
Antigen
Alternative Name CD178 (FASL Antibody Abstract)
Background The MFL4 antibody, generated against mouse CD178 (Fas Ligand, CD95 Ligand), cross-reacts with the rat Fas Ligand antigen. MFL4 mAb detects rat Fas Ligand expressed by transfected cells, but not the parental cell line. In addition, the epitope detected by MFL4 can be induced on activated rat splenic T lymphocytes. The MFL4 mAb has been reported to inhibit killing of mouse lymphoma line A20.2J by rat FasL-transfected cells. The tissue distribution of mouse Fas Ligand has been well characterized. It is expressed at the mRNA and protein levels in a variety of tissues and cell types, including spleen, bone marrow, testis, uterus, eye, and activated lymphocytes. The expression of Fas Ligand in the rat has been detected in liver Kupffer cells, T cells in the central nervous system, spleen, and corpus luteum. MFL4 antibody reacts with the mouse Fas Ligand protein in a pattern similar to that of MFL3 antibody (Cat. No. 555291). Fas Ligand is a type-II transmembrane protein, a member of the TNF/NGF superfamily and a ligand for Fas (CD95). Fas Ligand and its counter-receptor CD95 are believed to participate in T-cell development, the regulation of immune responses, and cell-mediated cytotoxic mechanisms. It has been reported that human Fas Ligand is released from the surface of activated lymphocytes by metalloproteinase.
Synonyms: Fas Ligand, CD95 Ligand
Pathways Apoptosis, EGFR Signaling Pathway

Application Details

Product Details anti-FASL Antibody Target Details FASL Handling References for anti-FASL Antibody (ABIN1176980) Images back to top
Application Notes We have found that enriched splenic T cells are induced to express Fas Ligand by 6 - 8-hour culture with plate-bound anti-alphabeta TCR mAb R73 (Cat. No. 554910) and anti-rat CD28 mAb JJ316 (Cat. No. 554992). Because Fas Ligand is expressed at low density on activated cells, we recommend the use of a biotinylated second-step antibody, such as polyclonal goat anti-hamster IgG, with a bright third-step reagent, such as Streptavidin-PE (Cat. No. 554061). Other reported applications include blocking of the cytotoxic activities of mFasL-transfected L5178Y T lymphoma, rFasL-transfected COS cells, and rFasL-transfected L5178Y cells.
Restrictions For Research Use only

Handling

Product Details anti-FASL Antibody Target Details FASL Application Details References for anti-FASL Antibody (ABIN1176980) Images back to top
Concentration 1.0 mg/mL
Buffer Aqueous buffered solution containing no preservative, 0.2μm filtered. Endotoxin level is ≤0.01 ng/μg of protein.
Preservative Azide free
Storage 4 °C
Storage Comment This preparation contains no preservatives, thus it should be handled under aseptic conditions. Store undiluted at 4°C.

References for anti-FASL Antibody (ABIN1176980)

Product Details anti-FASL Antibody Target Details FASL Application Details Handling Images back to top
Product cited in:

Trinité, Voisine, Yagita, Josien: "A subset of cytolytic dendritic cells in rat." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 165, Issue 8, pp. 4202-8, 2000

Moalem, Monsonego, Shani, Cohen, Schwartz: "Differential T cell response in central and peripheral nerve injury: connection with immune privilege." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 13, Issue 10, pp. 1207-17, 1999

Müschen, Warskulat, Douillard, Gilbert, Häussinger: "Regulation of CD95 (APO-1/Fas) receptor and ligand expression by lipopolysaccharide and dexamethasone in parenchymal and nonparenchymal rat liver cells." in: Hepatology (Baltimore, Md.), Vol. 27, Issue 1, pp. 200-8, 1998

White, McCombe, Pender: "The roles of Fas, Fas ligand and Bcl-2 in T cell apoptosis in the central nervous system in experimental autoimmune encephalomyelitis." in: Journal of neuroimmunology, Vol. 82, Issue 1, pp. 47-55, 1998

Kayagaki, Yamaguchi, Nagao, Matsuo, Maeda, Okumura, Yagita: "Polymorphism of murine Fas ligand that affects the biological activity." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 94, Issue 8, pp. 3914-9, 1997

Griffith, Brunner, Fletcher, Green, Ferguson: "Fas ligand-induced apoptosis as a mechanism of immune privilege." in: Science (New York, N.Y.), Vol. 270, Issue 5239, pp. 1189-92, 1996

Kayagaki, Kawasaki, Ebata, Ohmoto, Ikeda, Inoue, Yoshino, Okumura, Yagita: "Metalloproteinase-mediated release of human Fas ligand." in: The Journal of experimental medicine, Vol. 182, Issue 6, pp. 1777-83, 1996

Suda, Okazaki, Naito, Yokota, Arai, Ozaki, Nakao, Nagata: "Expression of the Fas ligand in cells of T cell lineage." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 154, Issue 8, pp. 3806-13, 1995

Brunner, Mogil, LaFace, Yoo, Mahboubi, Echeverri, Martin, Force, Lynch, Ware: "Cell-autonomous Fas (CD95)/Fas-ligand interaction mediates activation-induced apoptosis in T-cell hybridomas." in: Nature, Vol. 373, Issue 6513, pp. 441-4, 1995

Vignaux, Vivier, Malissen, Depraetere, Nagata, Golstein: "TCR/CD3 coupling to Fas-based cytotoxicity." in: The Journal of experimental medicine, Vol. 181, Issue 2, pp. 781-6, 1995

Smith, Farrah, Goodwin: "The TNF receptor superfamily of cellular and viral proteins: activation, costimulation, and death." in: Cell, Vol. 76, Issue 6, pp. 959-62, 1994

Images

Product Details anti-FASL Antibody Target Details FASL Application Details Handling References for anti-FASL Antibody (ABIN1176980) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-FASL antibody (Fas Ligand (TNF Superfamily, Member 6)) (ABIN1176980) anti-Fas Ligand (TNF Superfamily, Member 6) (FASL) antibody