|Application / Reactivity||Rat (Rattus)||Mouse (Murine)|
|Blocking Reagent (BR)||2 Antibodies||3 Antibodies|
|ELISA||5 Antibodies||7 Antibodies|
|Flow Cytometry (FACS)||15 Antibodies||90 Antibodies|
|Functional Studies (Func)||2 Antibodies||6 Antibodies|
|Immunocytochemistry (ICC)||2 Antibodies||8 Antibodies|
|Immunofluorescence (IF)||1 Antibodies||5 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||11 Antibodies||11 Antibodies|
|Immunohistochemistry (IHC)||10 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||2 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||16 Antibodies|
|Immunoprecipitation (IP)||2 Antibodies|
|Western Blotting (WB)||32 Antibodies|
|Immunochromatography (IC)||3 Antibodies|
|Antigen||Fas Ligand (TNF Superfamily, Member 6) (FASL) Antibodies|
|Reactivity||Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This FASL antibody is un-conjugated Alternatives|
Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC)
|6 references available|
|Supplier||Log in to see|
Product Details anti-FASL AntibodyTarget Details FASL Application Details Handling References for anti-FASL Antibody (ABIN2665564) Images
|Purification||The LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.|
|Sterility||0.2 μm filtered|
Endotoxin level is <0.1 EU/μg of the protein (<0.01 ng/μg of the protein) as determined by the LAL test.
Target Details FASLProduct Details anti-FASL Antibody Application Details Handling References for anti-FASL Antibody (ABIN2665564) Images back to top
|Alternative Name||CD178 (FASL Antibody Abstract)|
|Background||CD178 is a 40 kD member of the TNF/NGF superfamily also known as Fas ligand, FasL, Apo-1 ligand, and CD95 ligand. Cell surface CD178 is expressed on activated T cells and in testis and eye. CD178 is upregulated in activated T cells upon TCR re-engagement and has been shown to induce autocrine and paracrine T cell death. CD178 expression in the eye and testis has been shown to participate in immune privilege at these sites. CD178 can be cleaved from the surface by metalloproteases, and "soluble" CD178 may block the activities of membrane-bound CD178. CD178 binds to CD95 (Fas) to induce apoptotic cell death implicated in the maintenance of peripheral tolerance. CD178/CD95 interactions have also been implicated in the proliferation of CD8+ cells and neutrophil extravasation, chemotaxis, and survival. The MFL4 antibody recognizes CD178 in a wide array of mouse strains and has been reported to block CD178/CD95 induced apoptosis. The MFL4 antibody is cross-reactive with rat and mouse FasL.|
|Pathways||Apoptosis, EGFR Signaling Pathway|
Application DetailsProduct Details anti-FASL Antibody Target Details FASL Handling References for anti-FASL Antibody (ABIN2665564) Images back to top
|Application Notes||Optimal working dilution should be determined by the investigator.|
|Restrictions||For Research Use only|
HandlingProduct Details anti-FASL Antibody Target Details FASL Application Details References for anti-FASL Antibody (ABIN2665564) Images back to top
|Handling Advice||This LEAF™ solution contains no preservative, handle under aseptic conditions|
|Storage Comment||The antibody solution should be stored undiluted between 2°C and 8°C.|
References for anti-FASL Antibody (ABIN2665564)Product Details anti-FASL Antibody Target Details FASL Application Details Handling Images back to top
Chang, Chang, Lee et al.: "Cytopiloyne, a polyacetylenic glucoside, prevents type 1 diabetes in nonobese diabetic mice." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 178, Issue 11, pp. 6984-93, 2007 (PubMed).
Borges, Falcão, Leite-Júnior et al.: "Fas ligand triggers pulmonary silicosis." in: The Journal of experimental medicine, Vol. 194, Issue 2, pp. 155-64, 2001 (PubMed).
Trinité, Voisine, Yagita et al.: "A subset of cytolytic dendritic cells in rat." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 165, Issue 8, pp. 4202-8, 2000 (PubMed).
Hill, Ouhtit, Loughlin et al.: "Fas ligand: a sensor for DNA damage critical in skin cancer etiology." in: Science (New York, N.Y.), Vol. 285, Issue 5429, pp. 898-900, 1999 (PubMed).
Kayagaki, Yamaguchi, Nagao et al.: "Polymorphism of murine Fas ligand that affects the biological activity." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 94, Issue 8, pp. 3914-9, 1997 (PubMed).
Takahashi, Tanaka, Brannan et al.: "Generalized lymphoproliferative disease in mice, caused by a point mutation in the Fas ligand." in: Cell, Vol. 76, Issue 6, pp. 969-76, 1994 (PubMed).
ImagesProduct Details anti-FASL Antibody Target Details FASL Application Details Handling References for anti-FASL Antibody (ABIN2665564) back to top