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Human FASL ELISA Kit

Recommended FASL Product (supplied by: Log in to see )

Antigen
Fas Ligand (TNF Superfamily, Member 6) (FASL) ELISA Kits
  • ALPS1B
  • APT1LG1
  • Apt1Lg1
  • APTL
  • CD95-L
  • CD95L
  • CD178
  • FAS
  • Fas-L
  • FASL
  • fasl
  • Fasl
  • Faslg
  • FASLG
  • gld
  • TNFRSF6
  • Tnfsf6
  • TNFSF6
  • zgc:162027
Epitope
AA 134-281
Reactivity
Human
Alternatives
Kits with alternative reactivity to:
56
22
18
6
3
3
2
2
2
1
1
1
1
1
Methode Type
Sandwich ELISA
Detection Range
15.6-1000 pg/mL
Minimum Detection Limit
15.6 pg/mL
Application
ELISA
Supplier
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Catalog No. ABIN1672725
$ 420.00
Plus shipping costs $45.00
Relevance Score ABIN Method Type Sample Type Detection Range Detection Minimum Supplier References Details
0.32693866 ABIN414369 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids 15.63-1000 pg/mL 15.63 pg/mL Log in to see 1
0.32693866 ABIN924845 Sandwich ELISA Cell Lysate, Serum, Plasma 32-2000 pg/mL 32 pg/mL Log in to see
0.32693866 ABIN2681245 Sandwich ELISA Serum, Plasma, Cell Culture Supernatant 15.63-1000 pg/mL 15.63 pg/mL Log in to see
0.32693866 ABIN1114850 Sandwich ELISA Serum, Plasma, Biological Fluids 15.63-1000 pg/mL 15.63 pg/mL Log in to see
0.32693866 ABIN1685912 Sandwich ELISA Serum, Plasma, Cell Culture Supernatant, Urine 2-1000 pg/mL 2 pg/mL Log in to see
0.32693866 ABIN2708130 Sandwich ELISA Cell Culture Supernatant, Serum, Plasma 15.6-1000 pg/mL 15.6 pg/mL Log in to see
0.32693866 ABIN2113886 Cell Culture Supernatant, Serum, Plasma (EDTA - heparin - citrate) Log in to see
0.0012117105 ABIN366641 Sandwich ELISA Serum, Plasma, Cell Culture Supernatant 31.25-2000 pg/mL 31.25 pg/mL Log in to see 1
0.0012117105 ABIN3032918 Sandwich ELISA Cell Culture Supernatant, Plasma, Serum 1.4-1000 pg/mL 1.4 pg/mL Log in to see
0.0012117105 ABIN624973 Sandwich ELISA Serum, Plasma, Cell Culture Supernatant, Urine Log in to see 1
0.0012117105 ABIN510442 Sandwich ELISA Serum, Plasma, Cell Culture Supernatant, Tissue Homogenate, Body Fluids 50-1000 pg/mL 50 pg/mL Log in to see
0.0012117105 ABIN1446108 Cell Culture Supernatant, Serum, Plasma, Biological Samples 12 pg/mL Log in to see 1
0.0012117105 ABIN1029814 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids 15.625-1000 pg/mL 15.625 pg/mL Log in to see
0.0012117105 ABIN2684231 Sandwich ELISA Plasma (citrate), Plasma (EDTA), Plasma (heparin), Amniotic Fluid, Cell Culture Supernatant, Serum 0.16-10 ng/mL 0.16 ng/mL Log in to see 2
0.0012117105 ABIN1112601 Sandwich ELISA Serum, Plasma, Body Fluids, Tissue Lysate, Cell Culture Supernatant 15.6-1000 pg/mL 15.6 pg/mL Log in to see
0.0012117105 ABIN1381131 Cell ELISA Log in to see
0.0012117105 ABIN2115107 Cell Lysate, Serum, Plasma 32-2000 pg/mL 32 pg/mL Log in to see
0.0012117105 ABIN2684233 Sandwich ELISA Biological Fluids, Cell Culture Supernatant, Plasma, Serum 62.5-2000 pg/mL 62.5 pg/mL Log in to see
0.0012117105 ABIN2684232 Sandwich ELISA Body Fluids, Cell Culture Supernatant, Plasma, Serum, Tissue Lysate 15.6-1000 pg/mL 15.6 pg/mL Log in to see
0.0012117105 ABIN1540239 Cell Culture Supernatant, Serum, Plasma (EDTA - heparin - citrate) 15.6-1000 pg/mL 15.6 pg/mL Log in to see

Top referenced FASL ELISA Kit for ELISA

  • Human FASL ELISA Kit for Sandwich ELISA - ABIN1672725 (7 Publications): Asanuma, Tsuji, Endoh, Yagihashi, Watanabe: Survivin enhances Fas ligand expression via up-regulation of specificity protein 1-mediated gene transcription in colon cancer cells. in Journal of immunology (Baltimore, Md. : 1950) 2004 (PubMed)

  • Human FASL ELISA Kit for Sandwich ELISA - ABIN2684231 (2 Publications): Krasznai, Szaniszlo, Kraxner, Vargha, Kovacs, Borocz, Kaszas, Mezei, Cserhati, Cseh: Association of TLR-4 and TNF-alpha polymorphisms with clinical symptoms and cytokine levels in patients with allergic rhinitis. in European archives of oto-rhino-laryngology : official journal of the European Federation of Oto-Rhino-Laryngological Societies (EUFOS) : affiliated with the German Society for Oto-Rhino-Laryngology - Head and Neck Surgery 2011 (PubMed)

  • Human FASL ELISA Kit - ABIN1446108 (1 Publications): Kadam, Abhang: Serum levels of soluble Fas ligand, granzyme B and cytochrome c during adjuvant chemotherapy of breast cancer. in Clinica chimica acta; international journal of clinical chemistry 2014 (PubMed)

  • Human FASL ELISA Kit for Sandwich ELISA - ABIN624973 (1 Publications): Whitman, Barber: NKG2D receptor activation of NF-κB enhances inflammatory cytokine production in murine effector CD8(+) T cells. in Molecular immunology 2014 (PubMed)

  • Human FASL ELISA Kit for Sandwich ELISA - ABIN366641 (1 Publications): Wu, Chen, Fang, Xie, Hong, Xia: Elevated Fas/FasL system and endothelial cell microparticles are involved in endothelial damage in acute graft-versus-host disease: a clinical analysis. in Leukemia research 2012 (PubMed)

  • Human FASL ELISA Kit for Sandwich ELISA - ABIN414369 (1 Publications): Xu, Deng, He, Pan, Li, Gao, Sun, Song, Wang, Cho: The diplotype Fas -1377A/-670G as a genetic marker to predict a lower risk of breast cancer in Chinese women. in Tumour biology 2014 (PubMed)

Similar FASL ELISA Kits

Application / Reactivity Human
ELISA 39 ELISA Kits

General

Antigen Fas Ligand (TNF Superfamily, Member 6) (FASL) ELISA Kits
Epitope AA 134-281
Reactivity Human
Kits with alternative reactivity to:
(56), (22), (18), (6), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1)
Methode Type Sandwich ELISA
Detection Range 15.6-1000 pg/mL
Minimum Detection Limit 15.6 pg/mL
Application ELISA
Pubmed 7 references available
Supplier Log in to see

Product Details FASL ELISA Kit

Target details Application Details Handling References for FASL Kit (ABIN1672725) Images
Purpose For quantitative detection of human FASL in cell culture supernates, serum and plasma(heparin, EDTA, citrate).
Brand PicoKine™
Sample Type Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA), Plasma (citrate)
Detection Method Colorimetric
Specificity Natural and recombinant human FASL
Cross-Reactivity (Details) There is no detectable cross-reactivity with other relevant proteins.
Sensitivity <2pg/mL
Components
  • 96-well plate precoated with anti- human FASL antibod - 1
  • Lyophilized recombinant human FASL standar - 10ng/tubex2
  • Biotinylated anti- human FASL antibod - 130μl(dilution 1:100)
  • Avidin-Biotin-Peroxidase Complex (ABC - 130μl(dilution 1:100)
  • Sample diluent buffe - 30 ml
  • Antibody diluent buffe - 12ml
  • ABC diluent buffe - 12ml
  • TMB color developing agen - 10ml
  • TMB stop solutio - 10ml
Material not included Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
Immunogen Expression system for standard: CHO
Immunogen sequence: P134-L281

Target details

Product Details FASL ELISA Kit Application Details Handling References for FASL Kit (ABIN1672725) Images back to top
Antigen
Alternative Name FASLG (FASL ELISA Kit Abstract)
Background Fas ligand(FasL or CD95L) is a type-II transmembrane protein that belongs to the tumor necrosis factor(TNF) family. Its binding with its receptor induces apoptosis. The human FASL gene consists of approximately 8 kb and is split into 4 exons. Fas ligand/receptor interactions play an important role in the regulation of the immune system and the progression of cancer. Fas ligand or FasL is a homotrimeric type II transmembrane protein. It signals through trimerization of FasR, which spans the membrane of the "target" cell. This trimerization usually leads to apoptosis, or cell death. Soluble Fas ligand is generated by cleaving membrane-bound FasL at a conserved cleavage site by the external matrix metalloproteinase MMP-7.
Gene ID 14103
UniProt Q99PH8
Pathways Apoptosis, EGFR Signaling Pathway

Application Details

Product Details FASL ELISA Kit Target details Handling References for FASL Kit (ABIN1672725) Images back to top
Application Notes Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
Plate Pre-coated,Strips (12 x 8)
Protocol human FASL ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for FASL has been precoated onto 96-well plates. Standards(CHO, P134-L281) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for FASL is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human FASL amount of sample captured in plate.
Assay Procedure Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL, 15.6pg/mL human FASL standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA, citrate) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human FASL standard solution and each sample be measured in duplicate.
Assay Precision
  • Sample 1: n=16, Mean(pg/ml): 108, Standard deviation: 5.62, CV(%): 5.2
  • Sample 2: n=16, Mean(pg/ml): 257, Standard deviation: 12.59, CV(%): 4.9
  • Sample 3: n=16, Mean(pg/ml): 619, Standard deviation: 36.52, CV(%): 5.9,
  • Sample 1: n=24, Mean(pg/ml): 112, Standard deviation: 9.74, CV(%): 8.7
  • Sample 2: n=24, Mean(pg/ml): 249, Standard deviation: 22.66, CV(%): 9.1
  • Sample 3: n=24, Mean(pg/ml): 623, Standard deviation: 44.86, CV(%): 7.2
Restrictions For Research Use only

Handling

Product Details FASL ELISA Kit Target details Application Details References for FASL Kit (ABIN1672725) Images back to top
Handling Advice Avoid multiple freeze-thaw cycles.
Storage -20 °C,4 °C
Storage Comment Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
Expiry Date 12 months

References for FASL Kit (ABIN1672725)

Product Details FASL ELISA Kit Target details Application Details Handling Images back to top
Product cited in:

Xu, Liu, Wu, Gong, Zhou, Qiao: "Proapoptotic effect of metalloproteinase 9 secreted by trophoblasts on endothelial cells." in: The journal of obstetrics and gynaecology research, Vol. 37, Issue 3, pp. 187-94, 2011

Xiong, Lu, Zhao, Xu, Bao, Lin, Yang: "Therapy with FasL-gene-modified dendritic cells confers a protective microenvironment in murine pregnancy." in: Fertility and sterility, Vol. 93, Issue 8, pp. 2767-9, 2010

Li, Peng, Li, Liu, Liang, Zhang: "Clinical significance of Fas and FasL protein expression in gastric carcinoma and local lymph node tissues." in: World journal of gastroenterology, Vol. 16, Issue 10, pp. 1274-8, 2010

Han, Qiu, Zhang, Kong, Wang, Wang, Li, Duan, Wang, Song, Wang: "Transplantation of sertoli-islet cell aggregates formed by microgravity: prolonged survival in diabetic rats." in: Experimental biology and medicine (Maywood, N.J.), Vol. 234, Issue 5, pp. 595-603, 2009

Background publications

Nakamura, Imai, Matsumoto, Sato, Takeuchi, Igarashi, Harada, Azuma, Krust, Yamamoto, Nishina, Takeda, Takayanagi, Metzger, Kanno, Takaoka, Martin, Chambon, Kato: "Estrogen prevents bone loss via estrogen receptor alpha and induction of Fas ligand in osteoclasts." in: Cell, Vol. 130, Issue 5, pp. 811-23, 2007

Asanuma, Tsuji, Endoh, Yagihashi, Watanabe: "Survivin enhances Fas ligand expression via up-regulation of specificity protein 1-mediated gene transcription in colon cancer cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 172, Issue 6, pp. 3922-9, 2004

Hahne, Rimoldi, Schröter, Romero, Schreier, French, Schneider, Bornand, Fontana, Lienard, Cerottini, Tschopp: "Melanoma cell expression of Fas(Apo-1/CD95) ligand: implications for tumor immune escape." in: Science (New York, N.Y.), Vol. 274, Issue 5291, pp. 1363-6, 1996

Images

Product Details FASL ELISA Kit Target details Application Details Handling References for FASL Kit (ABIN1672725) back to top
Supplier Images
ELISA image for FASL ELISA Kit (Fas Ligand (TNF Superfamily, Member 6)) (ABIN1672725) Human FASL PicoKine ELISA Kit standard curve