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|Application / Reactivity||Human|
|Biochemical Assay (BCA)||2 Antibodies|
|Enzyme Immunoassay (EIA)||10 Antibodies|
|Flow Cytometry (FACS)||113 Antibodies|
|Immunochromatography (IC)||18 Antibodies|
|Immunocytochemistry (ICC)||81 Antibodies|
|Immunofluorescence (IF)||149 Antibodies|
|Immunofluorescence (Cultured Cells) (IF (cc))||11 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||25 Antibodies|
|Immunohistochemistry (IHC)||235 Antibodies|
|Immunohistochemistry (Formalin-fixed Sections) (IHC (f))||2 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||86 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||193 Antibodies|
|Immunoprecipitation (IP)||55 Antibodies|
|Antigen||Keratin 18 (KRT18) Antibodies|
|Reactivity||Human, Dog (Canine), Cat (Feline) Alternatives|
|Conjugate||This KRT18 antibody is un-conjugated Alternatives|
Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC), Western Blotting (WB)
|5 references available|
|Supplier||Log in to see|
Product Details anti-KRT18 AntibodyTarget Details KRT18 Application Details Handling References for anti-KRT18 Antibody (ABIN335220) Images
|Specificity||Human, canine and feline.|
|Immunogen||DE-K18 is a mouse monoclonal IgG1, kappa antibody derived by fusion of SP2/0 mouse myeloma cells with spleen cells from a (BALB/c x B6)F1 mouse immunized with a cytoskeletal preparation extracted from the human vulvar squamous carcinoma cell line A431.|
Target Details KRT18Product Details anti-KRT18 Antibody Application Details Handling References for anti-KRT18 Antibody (ABIN335220) Images back to top
|Alternative Name||Cytokeratin 18 / Keratin K18 (KRT18 Antibody Abstract)|
|Background||Cytokeratins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in human epithelial tissues by at least 20 different polypeptides. They range in molecular weight between 40 kDa and 68 kDa and isoelectric pH between 4.9 - 7.8. The individual human cytokeratins are numbered 1 to 20. The various epithelia in the human body usually express cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of maturation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays.|
|Pathways||Apoptosis, Caspase Cascade in Apoptosis|
Application DetailsProduct Details anti-KRT18 Antibody Target Details KRT18 Handling References for anti-KRT18 Antibody (ABIN335220) Images back to top
|Application Notes||DE-K18 reacts exclusively with cytokeratin 18 which is present in glandular epithelial cells of the digestive, respiratory, and urogenital tracts, endocrine and exocrine cells and mesothelial cells, as well as adenocarcinomas originating from them. DE-K18 is useful for immunocytochemistry on frozen en paraffin embedded tissues and with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent, immunoblotting and flow cytometry. For staining on paraffin-embedded tissues pretreatment with 0,1% pepsin in 0.1 N HCl 30 min at room temperature is required. Optimal antibody dilution should be determined by titration.|
|Restrictions||For Research Use only|
HandlingProduct Details anti-KRT18 Antibody Target Details KRT18 Application Details References for anti-KRT18 Antibody (ABIN335220) Images back to top
References for anti-KRT18 Antibody (ABIN335220)Product Details anti-KRT18 Antibody Target Details KRT18 Application Details Handling Images back to top
|Product cited in:||
Vos, van den Ingh, Ramaekers et al.: "The expression of keratins, vimentin, neurofilament proteins, smooth muscle actin, neuron-specific enolase, and synaptophysin in tumors of the specific glands in the canine anal region." in: Veterinary pathology, Vol. 30, Issue 4, pp. 352-61, 1993 (PubMed).
Bonfrer, Groeneveld, Korse et al.: "Monoclonal antibody M3 used in tissue polypeptide-specific antigen assay for the quantification of tissue polypeptide antigen recognizes keratin 18." in: Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine, Vol. 15, Issue 4, pp. 210-22, 1994 (PubMed).
van Bommel, Kenemans, Helmerhorst et al.: "Expression of cytokeratin 10, 13, and involucrin as prognostic factors in low stage squamous cell carcinoma of the uterine cervix." in: Cancer, Vol. 74, Issue 8, pp. 2314-20, 1994 (PubMed).
Ivanyi, Minke, Hageman et al.: "Patterns of expression of feline cytokeratins in healthy epithelia and mammary carcinoma cells." in: American journal of veterinary research, Vol. 53, Issue 3, pp. 304-14, 1992 (PubMed).
Vos, van den Ingh, de Neijs et al.: "Immunohistochemistry with keratin monoclonal antibodies in canine tissues: urogenital tract, respiratory tract, (neuro-)endocrine tissues, choroid plexus and spinal cord." in: Zentralblatt für Veterinärmedizin. Reihe A, Vol. 39, Issue 10, pp. 721-40, 1993 (PubMed).