anti-Human KRT18 antibody for Immunohistochemistry (Formalin-fixed Sections)

Recommended KRT18 Antibody (supplied by: Log in to see )

Antigen
Keratin 18 (KRT18) Antibodies
  • krt18
  • MGC64569
  • MGC75922
  • KRT18
  • CYK18
  • K18
  • CK18
  • Krt1-18
  • DreK18
  • cb83
  • dapk1
  • sb:cb83
  • wu:fa13f02
  • wu:fb36b04
  • krtt1c6
  • zgc:66015
  • keratin 18
  • similar to Keratin, type I cytoskeletal 18 (Cytokeratin-18) (CK-18) (Keratin-18) (K18)
  • Keratin, type I cytoskeletal 18
  • cytokeratin 18
  • K18, simple type I keratin
  • zgc:77517
  • krt18
  • KRT18
  • k1c18
  • LOC100008885
  • LOC100136778
  • Krt18
  • zgc:77517
Reactivity
Human
947
200
171
71
65
41
36
34
27
27
23
20
18
15
12
7
7
5
5
4
3
3
2
2
1
1
1
Host
Mouse
698
275
12
11
4
3
2
Clonality
Monoclonal
Conjugate
This KRT18 antibody is un-conjugated
52
48
22
18
17
17
14
14
14
14
14
14
14
14
14
11
11
8
4
3
3
3
3
3
3
3
3
2
2
2
1
Application
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
656
466
389
349
331
300
225
180
125
40
26
18
18
14
11
9
3
2
1
1
1
1
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Catalog No. ABIN1020183
$ 279.12
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN1020184 IHC (f) IHC (p) Mouse IgG1 kappa Log in to see DC-10

General

Antigen Keratin 18 (KRT18) Antibodies
Reactivity Human
(947), (200), (171), (71), (65), (41), (36), (34), (27), (27), (23), (20), (18), (15), (12), (7), (7), (5), (5), (4), (3), (3), (2), (2), (1), (1), (1)
Host Mouse
(698), (275), (12), (11), (4), (3), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This KRT18 antibody is un-conjugated
(52), (48), (22), (18), (17), (17), (14), (14), (14), (14), (14), (14), (14), (14), (14), (11), (11), (8), (4), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (1)
Application Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(656), (466), (389), (349), (331), (300), (225), (180), (125), (40), (26), (18), (18), (14), (11), (9), (3), (2), (1), (1), (1), (1)
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Product Details anti-KRT18 Antibody

Target Details KRT18 Application Details Handling Images
Purpose This antibody is designed for the specific localization of human cytokeratin 18 using IHC techniques in formalin-fixed, paraffin-embedded tissue sections.
Characteristics Cytokeratin 8 (CK8, 52 KDa) and Cytokeratin 18 (CK18, 45kDa) are typical cytokeratins of simple epithela and derived neoplasias. Usually, CK8 and CK18 are co-expressed but there are exceptions both in normal and abnormal tissue.Therefore it could be helpful to stain both cytokeratins separately to determine the keratin expression pattern more precisely. Simple secretory epithelia (both ductal and glandular) are positive for CK18 as well as the majority of adenocarcinomas, basal cell carcinomas and mesotheliomas. Negative for CK18 are stratified epithelia, squamous cell carcinomas, acanthocytes as well as all non-epithelial tumours. Immunohistochemistry (IHC) is a complex technique in which immunological and histological detection methods are combined. In general, the manipulation and processing of tissues before immunostaining, especially different types of tissue fixation and embedding, as well as the nature of the tissues themselves may cause inconsistent results (Nadji and Morales, 1983). Endogenous pseudoperoxidase and peroxidase activity or endogenous biotin and alkaline phosphatase activity can cause non-specific staining results depending on the detection system used. Tissues that contain Hepatitis B surface antigen (HBsAg) can produce false positives when using HRP detection systems (Omata et al, 1980). Insufficient contrast staining and/or improper mounting of the sample may influence the interpretation of results.
Purification Ready-to-use, Prediluted,
Material not included All reagents, materials, and laboratory equipment for IHC procedures are not provided with this antibody. This includes adhesive slides and cover slips, positive and negative control tissues, Xylene or adequate substitute, ethanol, distilled H2O, heat pretreatment equipment (pressure cooker, steamer, microwave), pipettes, Coplin jars, glass jars, moist chamber, histological baths, negative control reagents, counter-staining solution, mounting materials, and microscope.
Immunogen Human breast cancer cell line PMC-42.
Clone DC10
Isotype IgG1 kappa

Target Details KRT18

Product Details anti-KRT18 Antibody Application Details Handling Images back to top
Antigen
Alternative Name Cytokeratin 18 (KRT18 Antibody Abstract)
Research Area Signaling, Cytoskeleton, Extracellular Matrix
Pathways Apoptosis, Caspase Cascade in Apoptosis

Application Details

Product Details anti-KRT18 Antibody Target Details KRT18 Handling Images back to top
Comment

Staining pattern: Cytoplasmic. The interpretation of the stain results is the full responsibility of the user. Any experimental result must be confirmed by a medically established diagnostic product or procedure.
Positive control: Tissue sample from skin, breast or lung carcinoma.
External Negative Control: Tissue sample homologous to the test sample incubated with an antibody isotype not specific for cytokeratin 18.

Protocol Principles of the procedure: The demonstrations of antigens by IHC is a sequential procedure with several steps involving first the application of a specific antibody for the antigen of interest (primary antibody), then a secondary antibody which joins to the first, an enzyme complex, and the addition of a chromogenic substrate. The sample is washed between each step. Enzymatic activation of the chromogenic substrate creates a visible product where the antigen is located. The results are interpreted using a light microscope. The primary antibody can be used both in manual IHC and with automated immunostainers.

Paraffin-embedded tissue samples should be used. The antibody is also useful for immunostaining frozen tissue samples. Western blot techniques are not recommended.
Assay Procedure Antigen retrieval: HIER Citrate Buffer pH 6.5
Working dilution: (only for concentrates) 1:25 – 1:100
Incubation: 30 min, RT
Control Tissue: Skin, breast or lung carcinoma
Restrictions For Research Use only

Handling

Product Details anti-KRT18 Antibody Target Details KRT18 Application Details Images back to top
Format Liquid
Buffer The preparation contains saline buffer, stabilising and carriers proteins. (pH 7.4)
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store at 2-8 °C until the expiration date printed on product label. Do not use after the expiration date. If fresh solutions are required, these must be prepared immediately prior to use, and will be stable for at least one day at room temperature (20-25°C). Unused portion of antibody preparation should be discarded after one day.If the product is stored under different conditions from those stipulated in these technical indications, the new conditions must be verified by the user. The validity period of the ready to use products when opened, is the same as the expiration date indicated on the label of intact product.

Images

Product Details anti-KRT18 Antibody Target Details KRT18 Application Details Handling back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-Keratin 18 (KRT18) antibody (ABIN1020183) 10 × 20 × IHC of Cytokeratin 18 (clone DC10) on FFPE breast carcinoma. Citrate pH 6.5...