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anti-Mammalian KRT18 antibody for Enzyme Immunoassay

Recommended KRT18 Antibody (supplied by: Log in to see )

Antigen
Keratin 18 (KRT18) Antibodies
  • cb83
  • CK18
  • CYK18
  • dapk1
  • DreK18
  • K18
  • Krt1-18
  • krt18
  • KRT18
  • krtt1c6
  • MGC64569
  • MGC75922
  • sb:cb83
  • wu:fa13f02
  • wu:fb36b04
  • zgc:66015
Reactivity
Mammalian
901
168
143
57
55
33
29
25
20
19
19
18
13
12
7
7
7
5
4
3
3
3
1
1
1
Host
Mouse
666
268
10
9
6
2
Clonality
Monoclonal
Conjugate
This KRT18 antibody is un-conjugated
51
47
21
18
18
17
14
14
14
14
14
14
14
14
14
14
11
11
4
3
3
3
3
3
3
2
2
1
1
1
Application
Enzyme Immunoassay (EIA), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
626
447
378
339
312
273
252
194
164
34
18
11
11
9
3
2
2
1
1
1
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Catalog No. ABIN120625
$ 286.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008867729 ABIN371615 EIA IHC (fro) WB Mouse IgG2b N-Term Log in to see SB38b 7

Top referenced anti-KRT18 antibody for Enzyme Immunoassay

Similar anti-KRT18 Antibodies

Application / Reactivity Mammalian
ELISA 23 Antibodies
Enzyme Immunoassay (EIA) 2 Antibodies
Flow Cytometry (FACS) 30 Antibodies
Immunocytochemistry (ICC) 23 Antibodies
Immunofluorescence (IF) 20 Antibodies
Immunohistochemistry (IHC) 18 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 3 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 23 Antibodies
Immunoprecipitation (IP) 24 Antibodies
Western Blotting (WB) 27 Antibodies

General

Antigen Keratin 18 (KRT18) Antibodies
Reactivity Mammalian
(901), (168), (143), (57), (55), (33), (29), (25), (20), (19), (19), (18), (13), (12), (7), (7), (7), (5), (4), (3), (3), (3), (1), (1), (1)
Host Mouse
(666), (268), (10), (9), (6), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This KRT18 antibody is un-conjugated
(51), (47), (21), (18), (18), (17), (14), (14), (14), (14), (14), (14), (14), (14), (14), (14), (11), (11), (4), (3), (3), (3), (3), (3), (3), (2), (2), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
(626), (447), (378), (339), (312), (273), (252), (194), (164), (34), (18), (11), (11), (9), (3), (2), (2), (1), (1), (1)
Pubmed 6 references available
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Product Details anti-KRT18 Antibody

Target Details KRT18 Application Details Handling References for anti-KRT18 Antibody (ABIN120625) Images
Specificity The antibody C-04 reacts with Cytokeratin 18 (45 kDa).
Purification Affinity Chromatography on Protein A
Immunogen Cytoskeleton preparation of epidermal carcinoma cell line A431
Clone C-04
Isotype IgG1

Target Details KRT18

Product Details anti-KRT18 Antibody Application Details Handling References for anti-KRT18 Antibody (ABIN120625) Images back to top
Antigen
Alternative Name Cytokeratin 18 (KRT18 Antibody Abstract)
Background Cytokeratins are a member of intermediate filaments subfamily represented in epithelial tissues. Cytokeratins are a subfamily of intermediate filaments and characterized by remarkable biochemical diversity. Cytokeratins are represented in epithelial tissues by at least 20 different polypeptides, molecular weight between 40 kDa and 68 kDa. The individual cytokeratin polypeptides are designated 1 to 20 and divided into the type I (acidic cytokeratins 9-20) and type II (basic to neutral cytokeratins 1-8) families. Cytokeratins 18 belongs to type I family (acidic cytokeratins).Synonyms: CK18, CYK18, Cell proliferation-inducing gene 46 protein, Cytokeratin-18, K18, KRT18, Keratin 18, Keratin type I cytoskeletal 18, Keratin-18
Gene ID 3875
UniProt P05783
Research Area Signaling, Cytoskeleton, Extracellular Matrix
Pathways Apoptosis, Caspase Cascade in Apoptosis

Application Details

Product Details anti-KRT18 Antibody Target Details KRT18 Handling References for anti-KRT18 Antibody (ABIN120625) Images back to top
Application Notes Immunohistochemistry on paraffin sections. Flow Cytometry. Immunoprecipitation. Immunocytochemistry. ELISA: The antibody C-04 has been tested as the capture antibody in a sandwich ELISA for analysis of Cytokeratin 18 in combination with antibody DA-7 (cat. no. 1B-110-C100) and with the antibody DC-10 (cat. no. 1B-107-C100). Western Blot: 1-2 mg/mL, overnight in 4 °C, Resuspend approx. 50 mil. cells in 1 mLcold Lysis buffer (1 % laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Protease inhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysate with non-reducing SDS-PAGE sample buffer, Non-reducing conditions. SDS-PAGE (10 % separating gel). Positive control: HeLa human cervix carcinoma cell line, MCF-7 human breast adenocarcinoma cell line.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-KRT18 Antibody Target Details KRT18 Application Details References for anti-KRT18 Antibody (ABIN120625) Images back to top
Concentration 1.0 mg/mL
Buffer PBS, pH ~7.4 with 15 mM Sodium Azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf Life: one year from despatch.
Expiry Date 12 months

References for anti-KRT18 Antibody (ABIN120625)

Product Details anti-KRT18 Antibody Target Details KRT18 Application Details Handling Images back to top
Background publications

Cap, Cerman, Nemecek, Marekova, Hana, Frysak: "The influence of treatment with somatostatin analogues on morphology, proliferative and apoptotic activity in GH-secreting pituitary adenomas." in: Journal of clinical neuroscience : official journal of the Neurosurgical Society of Australasia, Vol. 10, Issue 4, pp. 444-8, 2003

Bártek, Vojt?sek, Stasková, Bártková, Kerekés, Rejthar, Kovarík: "A series of 14 new monoclonal antibodies to keratins: characterization and value in diagnostic histopathology." in: The Journal of pathology, Vol. 164, Issue 3, pp. 215-24, 1991

Taylor-Papadimitriou, Stampfer, Bartek, Lewis, Boshell, Lane, Leigh: "Keratin expression in human mammary epithelial cells cultured from normal and malignant tissue: relation to in vivo phenotypes and influence of medium." in: Journal of cell science, Vol. 94 ( Pt 3), pp. 403-13, 1990

Vojt?sek, Stasková, Nenutil, Lauerová, Kovarík, Rejthar, Bártková, Bártek: "Monoclonal antibodies recognizing different epitopes of cytokeratin No.18." in: Folia biologica, Vol. 35, Issue 6, pp. 373-82, 1990

Lauerová, Kovarik, Bártek, Rejthar, Vojt?sek: "Novel monoclonal antibodies defining epitope of human cytokeratin 18 molecule." in: Hybridoma, Vol. 7, Issue 5, pp. 495-504, 1989

Kovarík, Rejthar, Lauerová, Vojt?sek, Bártková: "Monoclonal antibodies against individual cytokeratins in the detection of metastatic spread." in: International journal of cancer. Supplement = Journal international du cancer. Supplement, Vol. 3, pp. 50-5, 1989