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|Application / Reactivity||Mammalian|
|Cytometry by Time of Flight (CyTOF)||1 Antibodies|
|Enzyme Immunoassay (EIA)||2 Antibodies|
|Flow Cytometry (FACS)||33 Antibodies|
|Immunocytochemistry (ICC)||21 Antibodies|
|Immunofluorescence (IF)||16 Antibodies|
|Immunofluorescence (fixed cells) (IF/ICC)||3 Antibodies|
|Immunohistochemistry (IHC)||18 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||6 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||26 Antibodies|
|Immunoprecipitation (IP)||15 Antibodies|
|Western Blotting (WB)||33 Antibodies|
|Antigen||Keratin 18 (KRT18) Antibodies|
|Conjugate||This KRT18 antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
|6 references available|
|Supplier||Log in to see|
Product Details anti-KRT18 AntibodyTarget Details KRT18 Application Details Handling References for anti-KRT18 Antibody (ABIN120625) Images
|Specificity||The antibody C-04 reacts with Cytokeratin 18 (45 kDa).|
|Purification||Affinity Chromatography on Protein A|
|Immunogen||Cytoskeleton preparation of epidermal carcinoma cell line A431|
Target Details KRT18Product Details anti-KRT18 Antibody Application Details Handling References for anti-KRT18 Antibody (ABIN120625) Images back to top
|Alternative Name||Cytokeratin 18 (KRT18 Antibody Abstract)|
|Background||Cytokeratins are a member of intermediate filaments subfamily represented in epithelial tissues. Cytokeratins are a subfamily of intermediate filaments and characterized by remarkable biochemical diversity. Cytokeratins are represented in epithelial tissues by at least 20 different polypeptides, molecular weight between 40 kDa and 68 kDa. The individual cytokeratin polypeptides are designated 1 to 20 and divided into the type I (acidic cytokeratins 9-20) and type II (basic to neutral cytokeratins 1-8) families. Cytokeratins 18 belongs to type I family (acidic cytokeratins).Synonyms: CK18, CYK18, Cell proliferation-inducing gene 46 protein, Cytokeratin-18, K18, KRT18, Keratin 18, Keratin type I cytoskeletal 18, Keratin-18|
|Research Area||Signaling, Cytoskeleton, Extracellular Matrix|
|Pathways||Apoptosis, Caspase Cascade in Apoptosis|
Application DetailsProduct Details anti-KRT18 Antibody Target Details KRT18 Handling References for anti-KRT18 Antibody (ABIN120625) Images back to top
Immunohistochemistry on paraffin sections. Flow Cytometry. Immunoprecipitation. Immunocytochemistry. ELISA: The antibody C-04 has been tested as the capture antibody in a sandwich ELISA for analysis of Cytokeratin 18 in combination with antibody DA-7 (cat. no. 1B-110-C100) and with the antibody DC-10 (cat. no. 1B-107-C100). Western Blot: 1-2 mg/mL, overnight in 4 °C, Resuspend approx. 50 mil. cells in 1 mLcold Lysis buffer (1 % laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Protease inhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysate with non-reducing SDS-PAGE sample buffer, Non-reducing conditions. SDS-PAGE (10 % separating gel). Positive control: HeLa human cervix carcinoma cell line, MCF-7 human breast adenocarcinoma cell line.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-KRT18 Antibody Target Details KRT18 Application Details References for anti-KRT18 Antibody (ABIN120625) Images back to top
|Buffer||PBS, pH ~7.4 with 15 mM Sodium Azide as preservative.|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Storage||4 °C/-20 °C|
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf Life: one year from despatch.
|Expiry Date||12 months|
References for anti-KRT18 Antibody (ABIN120625)Product Details anti-KRT18 Antibody Target Details KRT18 Application Details Handling Images back to top
Cap, Cerman, Nemecek, Marekova, Hana, Frysak: "The influence of treatment with somatostatin analogues on morphology, proliferative and apoptotic activity in GH-secreting pituitary adenomas." in: Journal of clinical neuroscience : official journal of the Neurosurgical Society of Australasia, Vol. 10, Issue 4, pp. 444-8, 2003
Bártek, Vojt?sek, Stasková, Bártková, Kerekés, Rejthar, Kovarík: "A series of 14 new monoclonal antibodies to keratins: characterization and value in diagnostic histopathology." in: The Journal of pathology, Vol. 164, Issue 3, pp. 215-24, 1991
Taylor-Papadimitriou, Stampfer, Bartek, Lewis, Boshell, Lane, Leigh: "Keratin expression in human mammary epithelial cells cultured from normal and malignant tissue: relation to in vivo phenotypes and influence of medium." in: Journal of cell science, Vol. 94 ( Pt 3), pp. 403-13, 1990
Vojt?sek, Stasková, Nenutil, Lauerová, Kovarík, Rejthar, Bártková, Bártek: "Monoclonal antibodies recognizing different epitopes of cytokeratin No.18." in: Folia biologica, Vol. 35, Issue 6, pp. 373-82, 1990
Lauerová, Kovarik, Bártek, Rejthar, Vojt?sek: "Novel monoclonal antibodies defining epitope of human cytokeratin 18 molecule." in: Hybridoma, Vol. 7, Issue 5, pp. 495-504, 1989
Kovarík, Rejthar, Lauerová, Vojt?sek, Bártková: "Monoclonal antibodies against individual cytokeratins in the detection of metastatic spread." in: International journal of cancer. Supplement = Journal international du cancer. Supplement, Vol. 3, pp. 50-5, 1989