anti-Mammalian KRT18 antibody for Flow Cytometry

Recommended KRT18 Antibody (supplied by: Log in to see )

Antigen
Keratin 18 (KRT18) Antibodies
  • krt18
  • MGC64569
  • MGC75922
  • KRT18
  • CYK18
  • K18
  • CK18
  • Krt1-18
  • DreK18
  • cb83
  • dapk1
  • sb:cb83
  • wu:fa13f02
  • wu:fb36b04
  • krtt1c6
  • zgc:66015
  • keratin 18
  • similar to Keratin, type I cytoskeletal 18 (Cytokeratin-18) (CK-18) (Keratin-18) (K18)
  • Keratin, type I cytoskeletal 18
  • cytokeratin 18
  • K18, simple type I keratin
  • zgc:77517
  • krt18
  • KRT18
  • k1c18
  • LOC100008885
  • LOC100136778
  • Krt18
  • zgc:77517
Reactivity
Human, Mammalian, Mouse (Murine)
949
200
172
71
65
40
36
34
27
27
23
20
18
15
12
7
7
5
5
4
3
3
2
2
1
1
1
Host
Mouse
698
277
12
11
4
3
2
Clonality
Monoclonal
Conjugate
This KRT18 antibody is un-conjugated
52
48
22
18
17
17
14
14
14
14
14
14
14
14
14
11
11
8
4
3
3
3
3
3
3
3
3
2
2
2
1
Application
Cytometry by Time of Flight (CyTOF), ELISA, Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
657
466
388
349
331
299
224
180
125
40
26
18
17
14
11
9
3
2
2
1
1
1
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Catalog No. ABIN268940
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
14.117655 ABIN94279 ICC FACS IHC (p) IP ELISA WB Mouse IgG1 Log in to see C-04 6
14.117655 ABIN192045 ELISA FACS ICC IHC (p) IP WB FITC Mouse IgG1 Log in to see C-04 6
14.117655 ABIN94280 ELISA FACS ICC IHC (p) IP WB Biotin Mouse IgG1 Log in to see C-04 6
14.117655 ABIN269268 FACS IHC IHC (p) WB Biotin Mouse IgG1 Log in to see C-04
1 ABIN120625 EIA FACS IF IHC (p) IP WB Mouse IgG1 Log in to see C-04 6
1 ABIN462409 Inhibition ELISA FACS ICC IHC (p) IP WB Mouse IgG1 Log in to see C-04
1 ABIN1723419 ICC FACS IHC (p) IP ELISA WB Mouse IgG1 Log in to see C-04
1 ABIN2963247 FACS IHC (p) Biotin Mouse IgG1 full length Log in to see C-04
1 ABIN4302594 ELISA FACS ICC IF IHC IHC (p) IP WB FITC Mouse IgG1 Log in to see C-04
1 ABIN231622 FACS FITC Mouse IgG1 Log in to see C-04
1 ABIN601706 ICC FACS IHC WB Mouse IgG1 Log in to see RGE 53
1 ABIN1955569 FACS IHC (p) Biotin Mouse IgG1 Log in to see C-04
1 ABIN4302611 FACS PE Mouse IgG1 Log in to see C-04
1 ABIN4302608 ELISA FACS ICC IF IHC IHC (p) WB DyLight 405 Mouse IgG1 Log in to see C-04
1 ABIN4302620 ELISA FACS ICC IF IHC IHC (p) WB DyLight 755 Mouse IgG1 Log in to see C-04
1 ABIN4302642 ELISA FACS ICC IF IHC IHC (p) WB Alexa Fluor 405 Mouse IgG1 Log in to see C-04
1 ABIN4302645 ELISA FACS ICC IF IHC IHC (p) WB Alexa Fluor 700 Mouse IgG1 Log in to see C-04
1 ABIN4302614 ELISA FACS ICC IF IHC IHC (p) IP WB PerCP Mouse IgG1 Log in to see C-04
1 ABIN4302605 ELISA FACS ICC IF IHC IHC (p) WB DyLight 350 Mouse IgG1 Log in to see C-04
1 ABIN4302623 ELISA FACS ICC IF IHC IHC (p) WB DyLight 680 Mouse IgG1 Log in to see C-04

General

Antigen Keratin 18 (KRT18) Antibodies
Reactivity Human, Mammalian, Mouse (Murine)
(949), (200), (172), (71), (65), (40), (36), (34), (27), (27), (23), (20), (18), (15), (12), (7), (7), (5), (5), (4), (3), (3), (2), (2), (1), (1), (1)
Host Mouse
(698), (277), (12), (11), (4), (3), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This KRT18 antibody is un-conjugated
(52), (48), (22), (18), (17), (17), (14), (14), (14), (14), (14), (14), (14), (14), (14), (11), (11), (8), (4), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (1)
Application Cytometry by Time of Flight (CyTOF), ELISA, Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
(657), (466), (388), (349), (331), (299), (224), (180), (125), (40), (26), (18), (17), (14), (11), (9), (3), (2), (2), (1), (1), (1)
Pubmed 1 reference available
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Product Details anti-KRT18 Antibody

Target Details KRT18 Application Details Handling References for anti-KRT18 antibody (ABIN268940) Images
Specificity The antibody C-04 reacts with Cytokeratin 18 (45 kDa), a member of intermediate filaments subfamily represented in epithelial tissues.
Purification Protein A purified
Immunogen Cytoskeleton preparation of epidermal carcinoma cell line A431.
Clone C-04
Isotype IgG1

Target Details KRT18

Product Details anti-KRT18 Antibody Application Details Handling References for anti-KRT18 antibody (ABIN268940) Images back to top
Antigen
Alternative Name Cytokeratin 18 (KRT18 Antibody Abstract)
Background Gene Symbol: KRT18
Gene ID 3875
Research Area Signaling, Cytoskeleton, Extracellular Matrix
Pathways Apoptosis, Caspase Cascade in Apoptosis

Application Details

Product Details anti-KRT18 Antibody Target Details KRT18 Handling References for anti-KRT18 antibody (ABIN268940) Images back to top
Application Notes Western Blot 1 - 2 μg/mL, Flow Cytometry 1:10-1:1000, ELISA 1:2000, Immunohistochemistry 1:10-1:500, Immunocytochemistry/Immunofluorescence 1:10-1:500, Immunoprecipitation 1:50, Immunohistochemistry-Paraffin 10 μg/mL, CyTOF-readyPredicted molecular weight: 45 kDa. Western Blot - Resuspend approx. 50 mil. cells in 1 mL cold Lysis buffer (1 % laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Protease inhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysate with non-reducing SDS-PAGE sample buffer. Application note: Non-reducing conditions. SDS-PAGE (10 % separating gel) ELISA - The antibody C-04 has been tested as the capture antibody in a sandwich ELISA for analysis of Cytokeratin 18 in combination with antibody DA-7 and with the antibody DC-10.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only

Handling

Product Details anti-KRT18 Antibody Target Details KRT18 Application Details References for anti-KRT18 antibody (ABIN268940) Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer PBS ( pH 7.4)
Buffer contains: 15 mM Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles
Storage 4 °C
Storage Comment Store at 4°C. Do not freeze.

References for anti-KRT18 antibody (ABIN268940)

Product Details anti-KRT18 Antibody Target Details KRT18 Application Details Handling Images back to top
Product cited in:

Kaenel, Antonijevic, Richter, Küchler, Sutter, Wotzkow, Strange, Andres: "Deregulated ephrin-B2 signaling in mammary epithelial cells alters the stem cell compartment and interferes with the epithelial differentiation pathway." in: International journal of oncology, Vol. 40, Issue 2, pp. 357-69, 2011 (Sample species: Mouse (Murine)). Further details: Immunohistochemistry (Paraffin-embedded Sections)

Images

Product Details anti-KRT18 Antibody Target Details KRT18 Application Details Handling References for anti-KRT18 antibody (ABIN268940) back to top
Supplier Images
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Keratin 18 (KRT18) antibody (ABIN268940) Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (C-04) [ABIN268940] - Human ki...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Keratin 18 (KRT18) antibody (ABIN268940) Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (C-04) [ABIN268940] - Human Colon
Western Blotting (WB) image for anti-Keratin 18 (KRT18) antibody (ABIN268940) Western Blot: Cytokeratin 18 Antibody (C-04) - Analysis of cytokeratin 18 in HeLa ce...
Flow Cytometry (FACS) image for anti-Keratin 18 (KRT18) antibody (ABIN268940) Flow Cytometry: Cytokeratin 18 Antibody (C-04) - Intracellular flow cytometry analys...