You are viewing an incomplete version of our website. Please click to reload the website as full version.

anti-Mammalian KRT18 antibody for Western Blotting

Recommended KRT18 Antibody (supplied by: Log in to see )

Antigen
Keratin 18 (KRT18) Antibodies
  • cb83
  • CK18
  • CYK18
  • dapk1
  • DreK18
  • K18
  • Krt1-18
  • krt18
  • KRT18
  • krtt1c6
  • MGC64569
  • MGC75922
  • sb:cb83
  • wu:fa13f02
  • wu:fb36b04
  • zgc:66015
Reactivity
Mammalian
583
111
98
30
18
17
17
13
11
9
8
6
5
5
4
3
3
3
3
3
3
1
1
1
Host
Mouse
358
258
10
9
6
2
Clonality
Monoclonal
Conjugate
This KRT18 antibody is un-conjugated
39
39
10
4
4
4
3
3
3
3
3
3
3
2
2
1
1
1
Application
Enzyme Immunoassay (EIA), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
424
245
200
173
149
120
96
92
61
34
18
11
11
3
2
2
1
1
1
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

It's quick and easy to submit your validation proposal. I want to validate this product

Learn more

Catalog No. ABIN120625
$ 286.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0011794439 ABIN371615 EIA IHC (fro) WB Mouse IgG2b N-Term Log in to see SB38b 7
0.0011794439 ABIN94279 ICC FACS IHC (p) IP ELISA WB Mouse IgG1 Log in to see C-04 6
0.0011794439 ABIN452377 IHC (fro) IHC (p) WB Mouse IgG1 multi Epitope Log in to see Ks18-04-18-27-18-5-9B1-18-174
0.0011794439 ABIN181159 IHC (fro) IF WB Mouse IgG1 Log in to see RGE53
0.0011794439 ABIN1723419 ICC FACS IHC (p) IP ELISA WB Mouse IgG1 Log in to see C-04
0.0011794439 ABIN269268 ICC FACS IF IHC (p) IP IHC ELISA WB Biotin Mouse IgG1 Log in to see C-04
0.0011794439 ABIN268940 ICC FACS IF IHC (p) IP IHC ELISA WB Mouse IgG1 Log in to see C-04
0.0011794439 ABIN3200135 ELISA FACS ICC IHC (p) IP WB Biotin Mouse IgG1 Log in to see C-04
0.0011794439 ABIN3200136 ELISA FACS ICC IHC (p) IP WB FITC Mouse IgG1 Log in to see C-04
0.0011794439 ABIN3200134 ELISA FACS ICC IHC (p) IP WB Mouse IgG1 Log in to see C-04
0.0011794439 ABIN3037912 ICC FACS IF IP IHC ELISA WB Biotin Mouse IgG1 Log in to see
0.0011794439 ABIN3037913 ICC FACS IF IP IHC ELISA WB FITC Mouse IgG1 Log in to see
0.0011794439 ABIN3037910 ICC FACS IF IP IHC ELISA WB Mouse IgG1 Log in to see

Top referenced anti-KRT18 antibody for Western Blotting

Similar anti-KRT18 Antibodies

Application / Reactivity Mammalian
ELISA 10 Antibodies
Enzyme Immunoassay (EIA) 2 Antibodies
Flow Cytometry (FACS) 16 Antibodies
Immunocytochemistry (ICC) 10 Antibodies
Immunofluorescence (IF) 7 Antibodies
Immunohistochemistry (IHC) 5 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 3 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 10 Antibodies
Immunoprecipitation (IP) 11 Antibodies
Western Blotting (WB) 14 Antibodies

General

Antigen Keratin 18 (KRT18) Antibodies
Reactivity Mammalian
(583), (111), (98), (30), (18), (17), (17), (13), (11), (9), (8), (6), (5), (5), (4), (3), (3), (3), (3), (3), (3), (1), (1), (1)
Host Mouse
(358), (258), (10), (9), (6), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This KRT18 antibody is un-conjugated
(39), (39), (10), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (2), (2), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
(424), (245), (200), (173), (149), (120), (96), (92), (61), (34), (18), (11), (11), (3), (2), (2), (1), (1), (1)
Pubmed 6 references available
Supplier Log in to see

Product Details anti-KRT18 Antibody

Target Details KRT18 Application Details Handling References for anti-KRT18 Antibody (ABIN120625) Images
Specificity The antibody C-04 reacts with Cytokeratin 18 (45 kDa).
Purification Affinity Chromatography on Protein A
Immunogen Cytoskeleton preparation of epidermal carcinoma cell line A431
Clone C-04
Isotype IgG1

Target Details KRT18

Product Details anti-KRT18 Antibody Application Details Handling References for anti-KRT18 Antibody (ABIN120625) Images back to top
Antigen
Alternative Name Cytokeratin 18 (KRT18 Antibody Abstract)
Background Cytokeratins are a member of intermediate filaments subfamily represented in epithelial tissues. Cytokeratins are a subfamily of intermediate filaments and characterized by remarkable biochemical diversity. Cytokeratins are represented in epithelial tissues by at least 20 different polypeptides, molecular weight between 40 kDa and 68 kDa. The individual cytokeratin polypeptides are designated 1 to 20 and divided into the type I (acidic cytokeratins 9-20) and type II (basic to neutral cytokeratins 1-8) families. Cytokeratins 18 belongs to type I family (acidic cytokeratins).Synonyms: CK18, CYK18, Cell proliferation-inducing gene 46 protein, Cytokeratin-18, K18, KRT18, Keratin 18, Keratin type I cytoskeletal 18, Keratin-18
Gene ID 3875
UniProt P05783
Research Area Signaling, Cytoskeleton, Extracellular Matrix
Pathways Apoptosis, Caspase Cascade in Apoptosis

Application Details

Product Details anti-KRT18 Antibody Target Details KRT18 Handling References for anti-KRT18 Antibody (ABIN120625) Images back to top
Application Notes Immunohistochemistry on paraffin sections. Flow Cytometry. Immunoprecipitation. Immunocytochemistry. ELISA: The antibody C-04 has been tested as the capture antibody in a sandwich ELISA for analysis of Cytokeratin 18 in combination with antibody DA-7 (cat. no. 1B-110-C100) and with the antibody DC-10 (cat. no. 1B-107-C100). Western Blot: 1-2 mg/mL, overnight in 4 °C, Resuspend approx. 50 mil. cells in 1 mLcold Lysis buffer (1 % laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Protease inhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysate with non-reducing SDS-PAGE sample buffer, Non-reducing conditions. SDS-PAGE (10 % separating gel). Positive control: HeLa human cervix carcinoma cell line, MCF-7 human breast adenocarcinoma cell line.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-KRT18 Antibody Target Details KRT18 Application Details References for anti-KRT18 Antibody (ABIN120625) Images back to top
Concentration 1.0 mg/mL
Buffer PBS, pH ~7.4 with 15 mM Sodium Azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf Life: one year from despatch.
Expiry Date 12 months

References for anti-KRT18 Antibody (ABIN120625)

Product Details anti-KRT18 Antibody Target Details KRT18 Application Details Handling Images back to top
Background publications

Cap, Cerman, Nemecek, Marekova, Hana, Frysak: "The influence of treatment with somatostatin analogues on morphology, proliferative and apoptotic activity in GH-secreting pituitary adenomas." in: Journal of clinical neuroscience : official journal of the Neurosurgical Society of Australasia, Vol. 10, Issue 4, pp. 444-8, 2003

Bártek, Vojt?sek, Stasková, Bártková, Kerekés, Rejthar, Kovarík: "A series of 14 new monoclonal antibodies to keratins: characterization and value in diagnostic histopathology." in: The Journal of pathology, Vol. 164, Issue 3, pp. 215-24, 1991

Taylor-Papadimitriou, Stampfer, Bartek, Lewis, Boshell, Lane, Leigh: "Keratin expression in human mammary epithelial cells cultured from normal and malignant tissue: relation to in vivo phenotypes and influence of medium." in: Journal of cell science, Vol. 94 ( Pt 3), pp. 403-13, 1990

Vojt?sek, Stasková, Nenutil, Lauerová, Kovarík, Rejthar, Bártková, Bártek: "Monoclonal antibodies recognizing different epitopes of cytokeratin No.18." in: Folia biologica, Vol. 35, Issue 6, pp. 373-82, 1990

Lauerová, Kovarik, Bártek, Rejthar, Vojt?sek: "Novel monoclonal antibodies defining epitope of human cytokeratin 18 molecule." in: Hybridoma, Vol. 7, Issue 5, pp. 495-504, 1989

Kovarík, Rejthar, Lauerová, Vojt?sek, Bártková: "Monoclonal antibodies against individual cytokeratins in the detection of metastatic spread." in: International journal of cancer. Supplement = Journal international du cancer. Supplement, Vol. 3, pp. 50-5, 1989