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anti-Cow (Bovine) Lamin A/C antibody for Flow Cytometry

Recommended Lamin A/C Antibody (supplied by: Log in to see )

Antigen
Lamin A/C (LMNA) Antibodies
  • 74/76
  • 2459
  • cb948
  • cdcd1
  • CDCD1
  • CDDC
  • cddc
  • CG6944
  • CMD1A
  • cmd1a
  • cmt2b1
  • CMT2B1
  • D5
  • Dhe
  • Dm
  • Dm(0)
  • Dm0
  • Dm1
  • Dm2
  • Dmel\\CG6944
  • DmLamin
  • DmO
  • Dmo
  • Dm[[0]]
  • Dm[[1]]
  • Dm[[2]]
  • Dm[[mit]]
  • DM[[O]]
  • Dm[[o]]
  • emd2
  • EMD2
  • fk66d12
  • FPL
  • fpl
  • fpld
  • FPLD
  • FPLD2
  • HGPS
  • hgps
  • idc
  • IDC
  • jf27
  • l(2)04643
  • l(2)25Ec
  • l(2)gdh-7
  • l(2)gdh7
  • l(2)jf27
  • LAM
  • lam
  • Lam(Dm0)
  • lamDm0
  • LamDm[[0]]
  • lamDm[[0]]
  • Lam Dm[[0]]
  • LamDm[[o]]
  • lamin
  • Lamin-A
  • lamin-L(III)-like
  • lamin Dm0
  • lamin Dm[[0]]
  • Lamin Dm[[0]]
  • Lam[[Dm0]]
  • ldp1
  • LDP1
  • LFP
  • lfp
  • lgmd1b
  • LGMD1B
  • lmn1
  • LMN1
  • lmna-A
  • LMNC
  • lmnc
  • LMNL1
  • misg
  • nlam
  • PRO1
  • pro1
  • wu:fk66d12
Reactivity
Cow (Bovine), Dog (Canine), Human, Mouse (Murine), Rat (Rattus)
291
144
128
29
25
15
10
10
8
5
4
4
3
3
1
1
1
1
1
1
Host
Mouse
206
110
3
1
Clonality
Monoclonal
Conjugate
This Lamin A/C antibody is un-conjugated
5
4
4
3
2
2
2
2
2
2
2
2
2
2
2
1
1
Application
Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
272
107
98
77
60
57
33
31
30
23
11
2
2
2
2
2
1
1
Supplier
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Catalog No. ABIN112042
$ 390.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0010666476 ABIN108550 ICC FACS IHC (fro) IF IHC (p) WB Mouse IgG3 kappa AA 598-611 Log in to see 133A2 2
0.0010666476 ABIN112346 FACS IHC (fro) IF WB Mouse IgG1 Log in to see 131C3 3
0.0010666476 ABIN335242 ICC FACS IHC (fro) IHC WB Mouse IgG3 Log in to see 133A2 10
0.0010666476 ABIN335243 ICC FACS IHC (fro) IHC WB Mouse IgG1 Log in to see 131C3 3
0.0010666476 ABIN335389 ICC FACS IHC (fro) IHC WB Mouse IgG3 Log in to see 133A2 10
0.0010666476 ABIN335390 ICC FACS IHC (fro) IHC WB Mouse IgG1 Log in to see 131C3 3
0.0010666476 ABIN2963328 ICC IHC (fro) FACS IHC (p) WB Mouse IgG3 Log in to see 133A2

Top referenced anti-Lamin A/C antibody for Flow Cytometry

Similar anti-Lamin A/C Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine)
ELISA 35 Antibodies 35 Antibodies
Enzyme Immunoassay (EIA) 1 Antibodies 1 Antibodies
Flow Cytometry (FACS) 10 Antibodies 11 Antibodies
Immunochromatography (IC) 4 Antibodies 3 Antibodies
Immunocytochemistry (ICC) 25 Antibodies 27 Antibodies
Immunofluorescence (IF) 34 Antibodies
Immunofluorescence (Cultured Cells) (IF (cc)) 1 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 23 Antibodies
Immunohistochemistry (IHC) 52 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 12 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 26 Antibodies
Immunoprecipitation (IP) 5 Antibodies
Western Blotting (WB) 100 Antibodies
Blocking Reagent (BR) 2 Antibodies

General

Antigen Lamin A/C (LMNA) Antibodies
Reactivity Cow (Bovine), Dog (Canine), Human, Mouse (Murine), Rat (Rattus)
(291), (144), (128), (29), (25), (15), (10), (10), (8), (5), (4), (4), (3), (3), (1), (1), (1), (1), (1), (1)
Host Mouse
(206), (110), (3), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Lamin A/C antibody is un-conjugated
(5), (4), (4), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1)
Application Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(272), (107), (98), (77), (60), (57), (33), (31), (30), (23), (11), (2), (2), (2), (2), (2), (1), (1)
Pubmed 12 references available
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Product Details anti-Lamin A/C Antibody

Target Details Lamin A/C Application Details Handling References for anti-Lamin A/C Antibody (ABIN112042) Images
Specificity The antibody 133A2 recognizes an epitope located between residues 598-611 of Lamin A, therefore it reacts exclusively with Lamin A.
Purification Purified
Immunogen Partially purified recombinant Human Lamin A.
Clone 133A2
Isotype IgG3

Target Details Lamin A/C

Product Details anti-Lamin A/C Antibody Application Details Handling References for anti-Lamin A/C Antibody (ABIN112042) Images back to top
Antigen
Alternative Name Lamin-A/C (LMNA) (LMNA Antibody Abstract)
Background Nuclear lamins form a network of intermediate-type filaments at the nucleoplasmic site of the nuclear membrane. Two main subtypes of nuclear lamins can be distinguished, i.e. A-type lamins and B-type lamins. The A-type lamins comprise a set of three proteins arising from the same gene by alternative splicing, i.e. lamin A, lamin C and lamin Adel 10, while the B-type lamins include two proteins arising from two distinct genes, i.e. lamin B1 and lamin B2. Recent evidence has revealed that mutations in A-type lamins give rise to a range of rare but dominant genetic disorders, including Emery-Dreifuss muscular dystrophy, dilated cardiomyopathy with conduction-system disease and Dunnigan-type familial partial lipodystrophy. In addition, the expression of A-type lamins coincides with cell differentiation and as A-type lamins specifically interact with chromatin, a role in the regulation of differential gene expression has been suggested for A-type lamins.Synonyms: 70 kDa Lamin, LMN1, LMNA, Lamin A, Lamin A + C, Lamin-A/C, NY-REN-32, NYREN32, Nuclear Envelope Marker, Renal carcinoma antigen NY-REN-32
Molecular Weight 74 kDa (Predicted)
Gene ID 4000
NCBI Accession NP_005563
UniProt P02545
Research Area Cytoskeleton
Pathways Apoptosis, Caspase Cascade in Apoptosis

Application Details

Product Details anti-Lamin A/C Antibody Target Details Lamin A/C Handling References for anti-Lamin A/C Antibody (ABIN112042) Images back to top
Application Notes Immunocytochemistry. Immunohistochemistry on frozen sections (1: 100 - 1: 200 with avidin-biotinylatedhorseradish peroxidase complex (ABC) as detection reagent). Immunoblotting (1: 100 - 1: 1000). Flow cytometry (1: 100 - 1: 200).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Protocol Immunofluorescence protocol - Formaldehyde fixation1. Collect cells from T. c. unit and remove media from petri dish using suction. 2. Wash with 1x PBS and remove. 3. Incubate cells in pre-warm (37°C) Para-Formaldehyde for 12 minutes at roomtemperature on an orbital shaker. 4. Remove PFA and incubate in 0. 5% Triton X-IOO in 1x PBS for 5 minutes at roomtemperature. 5. Prepare blocking reagent, this is also the antibody diluent. 6. Wash cells 2x with 1x PBS at room temperature, for 4 minutes/wash on an orbital shaker. 7. Block with 1 % NCS and 1x PBS for 30 minutes at room temperature. 8. Prepare primary antibodies (50μl/coverslip) and moist staining chambers. 9. Wash cells 2x with lx PBS at room temperature and air dry briefly.
Restrictions For Research Use only

Handling

Product Details anti-Lamin A/C Antibody Target Details Lamin A/C Application Details References for anti-Lamin A/C Antibody (ABIN112042) Images back to top
Concentration 1.0 mg/mL
Buffer PBS containing 0.09 % Sodium Azide as preservative
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

References for anti-Lamin A/C Antibody (ABIN112042)

Product Details anti-Lamin A/C Antibody Target Details Lamin A/C Application Details Handling Images back to top
Background publications

Broers, Bronnenberg, Kuijpers, Schutte, Hutchison, Ramaekers: "Partial cleavage of A-type lamins concurs with their total disintegration from the nuclear lamina during apoptosis." in: European journal of cell biology, Vol. 81, Issue 12, pp. 677-91, 2003

De Sandre-Giovannoli, Bernard, Cau, Navarro, Amiel, Boccaccio, Lyonnet, Stewart, Munnich, Le Merrer, Lévy: "Lamin a truncation in Hutchinson-Gilford progeria." in: Science (New York, N.Y.), Vol. 300, Issue 5628, pp. 2055, 2003

Eriksson, Brown, Gordon, Glynn, Singer, Scott, Erdos, Robbins, Moses, Berglund, Dutra, Pak, Durkin, Csoka, Boehnke, Glover, Collins: "Recurrent de novo point mutations in lamin A cause Hutchinson-Gilford progeria syndrome." in: Nature, Vol. 423, Issue 6937, pp. 293-8, 2003

Broers, Machiels, van Eys, Kuijpers, Manders, van Driel, Ramaekers: "Dynamics of the nuclear lamina as monitored by GFP-tagged A-type lamins." in: Journal of cell science, Vol. 112 ( Pt 20), pp. 3463-75, 2000

Neri, Raymond, Giordano, Capitani, Martelli: "Lamin A is part of the internal nucleoskeleton of human erythroleukemia cells." in: Journal of cellular physiology, Vol. 178, Issue 3, pp. 284-95, 1999

Neri, Raymond, Giordano, Borgatti, Marchisio, Capitani, Martelli: "Spatial distribution of lamin A and B1 in the K562 cell nuclear matrix stabilized with metal ions." in: Journal of cellular biochemistry, Vol. 75, Issue 1, pp. 36-45, 1999

Broers, Machiels, Kuijpers, Smedts, van den Kieboom, Raymond, Ramaekers: "A- and B-type lamins are differentially expressed in normal human tissues." in: Histochemistry and cell biology, Vol. 107, Issue 6, pp. 505-17, 1997

Machiels, Ramaekers, Kuijpers, Groenewoud, Oosterhuis, Looijenga: "Nuclear lamin expression in normal testis and testicular germ cell tumours of adolescents and adults." in: The Journal of pathology, Vol. 182, Issue 2, pp. 197-204, 1997

Pugh, Coates, Lane, Raymond, Quinlan: "Distinct nuclear assembly pathways for lamins A and C lead to their increase during quiescence in Swiss 3T3 cells." in: Journal of cell science, Vol. 110 ( Pt 19), pp. 2483-93, 1997

Jansen, Machiels, Hopman, Broers, Bot, Arends, Ramaekers, Schouten: "Comparison of A and B-type lamin expression in reactive lymph nodes and nodular sclerosing Hodgkin's disease." in: Histopathology, Vol. 31, Issue 4, pp. 304-12, 1997

Machiels, Broers, Raymond, de Ley, Kuijpers, Caberg, Ramaekers: "Abnormal A-type lamin organization in a human lung carcinoma cell line." in: European journal of cell biology, Vol. 67, Issue 4, pp. 328-35, 1996

Hozák, Sasseville, Raymond, Cook: "Lamin proteins form an internal nucleoskeleton as well as a peripheral lamina in human cells." in: Journal of cell science, Vol. 108 ( Pt 2), pp. 635-44, 1995