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|Application / Reactivity||Rat (Rattus)||Mouse (Murine)|
|ELISA||35 Antibodies||35 Antibodies|
|Enzyme Immunoassay (EIA)||1 Antibodies||1 Antibodies|
|Flow Cytometry (FACS)||10 Antibodies||11 Antibodies|
|Immunochromatography (IC)||4 Antibodies||3 Antibodies|
|Immunocytochemistry (ICC)||25 Antibodies||27 Antibodies|
|Immunofluorescence (IF)||34 Antibodies|
|Immunofluorescence (Cultured Cells) (IF (cc))||2 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||24 Antibodies|
|Immunohistochemistry (IHC)||52 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||12 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||26 Antibodies|
|Immunoprecipitation (IP)||5 Antibodies|
|Western Blotting (WB)||100 Antibodies|
|Blocking Reagent (BR)||2 Antibodies|
|Antigen||Lamin A/C (LMNA) Antibodies|
|Reactivity||Cow (Bovine), Dog (Canine), Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This Lamin A/C antibody is un-conjugated Alternatives|
Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
|12 references available|
|Supplier||Log in to see|
Product Details anti-Lamin A/C AntibodyTarget Details Lamin A/C Application Details Handling References for anti-Lamin A/C Antibody (ABIN112042) Images
|Specificity||The antibody 133A2 recognizes an epitope located between residues 598-611 of Lamin A, therefore it reacts exclusively with Lamin A.|
|Immunogen||Partially purified recombinant Human Lamin A.|
Target Details Lamin A/CProduct Details anti-Lamin A/C Antibody Application Details Handling References for anti-Lamin A/C Antibody (ABIN112042) Images back to top
|Alternative Name||Lamin-A/C (LMNA) (LMNA Antibody Abstract)|
|Background||Nuclear lamins form a network of intermediate-type filaments at the nucleoplasmic site of the nuclear membrane. Two main subtypes of nuclear lamins can be distinguished, i.e. A-type lamins and B-type lamins. The A-type lamins comprise a set of three proteins arising from the same gene by alternative splicing, i.e. lamin A, lamin C and lamin Adel 10, while the B-type lamins include two proteins arising from two distinct genes, i.e. lamin B1 and lamin B2. Recent evidence has revealed that mutations in A-type lamins give rise to a range of rare but dominant genetic disorders, including Emery-Dreifuss muscular dystrophy, dilated cardiomyopathy with conduction-system disease and Dunnigan-type familial partial lipodystrophy. In addition, the expression of A-type lamins coincides with cell differentiation and as A-type lamins specifically interact with chromatin, a role in the regulation of differential gene expression has been suggested for A-type lamins.Synonyms: 70 kDa Lamin, LMN1, LMNA, Lamin A, Lamin A + C, Lamin-A/C, NY-REN-32, NYREN32, Nuclear Envelope Marker, Renal carcinoma antigen NY-REN-32|
|Molecular Weight||74 kDa (Predicted)|
|Pathways||Apoptosis, Caspase Cascade in Apoptosis|
Application DetailsProduct Details anti-Lamin A/C Antibody Target Details Lamin A/C Handling References for anti-Lamin A/C Antibody (ABIN112042) Images back to top
Immunocytochemistry. Immunohistochemistry on frozen sections (1: 100 - 1: 200 with avidin-biotinylatedhorseradish peroxidase complex (ABC) as detection reagent). Immunoblotting (1: 100 - 1: 1000). Flow cytometry (1: 100 - 1: 200).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Protocol||Immunofluorescence protocol - Formaldehyde fixation1. Collect cells from T. c. unit and remove media from petri dish using suction. 2. Wash with 1x PBS and remove. 3. Incubate cells in pre-warm (37°C) Para-Formaldehyde for 12 minutes at roomtemperature on an orbital shaker. 4. Remove PFA and incubate in 0. 5% Triton X-IOO in 1x PBS for 5 minutes at roomtemperature. 5. Prepare blocking reagent, this is also the antibody diluent. 6. Wash cells 2x with 1x PBS at room temperature, for 4 minutes/wash on an orbital shaker. 7. Block with 1 % NCS and 1x PBS for 30 minutes at room temperature. 8. Prepare primary antibodies (50μl/coverslip) and moist staining chambers. 9. Wash cells 2x with lx PBS at room temperature and air dry briefly.|
|Restrictions||For Research Use only|
HandlingProduct Details anti-Lamin A/C Antibody Target Details Lamin A/C Application Details References for anti-Lamin A/C Antibody (ABIN112042) Images back to top
|Buffer||PBS containing 0.09 % Sodium Azide as preservative|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.|
References for anti-Lamin A/C Antibody (ABIN112042)Product Details anti-Lamin A/C Antibody Target Details Lamin A/C Application Details Handling Images back to top
Eriksson, Brown, Gordon et al.: "Recurrent de novo point mutations in lamin A cause Hutchinson-Gilford progeria syndrome." in: Nature, Vol. 423, Issue 6937, pp. 293-8, 2003 (PubMed).
De Sandre-Giovannoli, Bernard, Cau et al.: "Lamin a truncation in Hutchinson-Gilford progeria." in: Science (New York, N.Y.), Vol. 300, Issue 5628, pp. 2055, 2003 (PubMed).
Broers, Bronnenberg, Kuijpers et al.: "Partial cleavage of A-type lamins concurs with their total disintegration from the nuclear lamina during apoptosis." in: European journal of cell biology, Vol. 81, Issue 12, pp. 677-91, 2003 (PubMed).
Broers, Machiels, van Eys et al.: "Dynamics of the nuclear lamina as monitored by GFP-tagged A-type lamins." in: Journal of cell science, Vol. 112 ( Pt 20), pp. 3463-75, 2000 (PubMed).
Neri, Raymond, Giordano et al.: "Spatial distribution of lamin A and B1 in the K562 cell nuclear matrix stabilized with metal ions." in: Journal of cellular biochemistry, Vol. 75, Issue 1, pp. 36-45, 1999 (PubMed).
Neri, Raymond, Giordano et al.: "Lamin A is part of the internal nucleoskeleton of human erythroleukemia cells." in: Journal of cellular physiology, Vol. 178, Issue 3, pp. 284-95, 1999 (PubMed).
Pugh, Coates, Lane et al.: "Distinct nuclear assembly pathways for lamins A and C lead to their increase during quiescence in Swiss 3T3 cells." in: Journal of cell science, Vol. 110 ( Pt 19), pp. 2483-93, 1997 (PubMed).
Jansen, Machiels, Hopman et al.: "Comparison of A and B-type lamin expression in reactive lymph nodes and nodular sclerosing Hodgkin's disease." in: Histopathology, Vol. 31, Issue 4, pp. 304-12, 1997 (PubMed).
Machiels, Ramaekers, Kuijpers et al.: "Nuclear lamin expression in normal testis and testicular germ cell tumours of adolescents and adults." in: The Journal of pathology, Vol. 182, Issue 2, pp. 197-204, 1997 (PubMed).
Broers, Machiels, Kuijpers et al.: "A- and B-type lamins are differentially expressed in normal human tissues." in: Histochemistry and cell biology, Vol. 107, Issue 6, pp. 505-17, 1997 (PubMed).
Machiels, Broers, Raymond et al.: "Abnormal A-type lamin organization in a human lung carcinoma cell line." in: European journal of cell biology, Vol. 67, Issue 4, pp. 328-35, 1996 (PubMed).
Hozák, Sasseville, Raymond et al.: "Lamin proteins form an internal nucleoskeleton as well as a peripheral lamina in human cells." in: Journal of cell science, Vol. 108 ( Pt 2), pp. 635-44, 1995 (PubMed).