|Antigen||Lamin A/C (LMNA) Antibodies|
|Reactivity||Cow (Bovine), Fish, Human, Mouse (Murine), Rat (Rattus), Xenopus laevis, Zebrafish (Danio rerio) Alternatives|
|Conjugate||This Lamin A/C antibody is un-conjugated Alternatives|
Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Western Blotting (WB)
|6 references available|
|Supplier||Log in to see|
Product Details anti-Lamin A/C AntibodyTarget Details Lamin A/C Application Details Handling References for anti-Lamin A/C antibody (ABIN452378) Images
|Specificity||The monoclonal antibody decorates the lamina, i.e. the nucleoplasmic layer coating the nuclear envelope. Mutations in lamin A have been shown to cause Hutchinson-Gilford progeria syndrome in Human and Mouse and other laminopathies (for review see Burke & Stewart, 2002). Polypeptide(s) Reacting: Lamin isotypes A and C (Mr 60-75 kDa), meiotic lamin C2. Tested Reactivity on Cultured Cell Lines: RV-SMC (Rat Vascular Smooth Muscle Cells).|
|Cross-Reactivity (Details)||Species reactivity (tested):Xenopus laevis (LA), Human (LA, C), Bovine (LA, C), Mouse (LA, C), Rat (LA, C), Trout (LA), Zebrafish (LA).|
|Characteristics||Synonyms: LMNA, LMN1, 70 kDa Lamin, NY-REN-32, NYREN32, Lamin-A/C, Lamin A, Lamin A + C,Nuclear Envelope Marker, Renal carcinoma antigen NY-REN-32|
|Immunogen||Nuclear pore complex-lamina fraction of Bovine liver.|
Target Details Lamin A/CProduct Details anti-Lamin A/C Antibody Application Details Handling References for anti-Lamin A/C antibody (ABIN452378) Images back to top
|Alternative Name||Lamin-A/C (LMNA) (LMNA Antibody Abstract)|
|Background||Lamin A and lamin C are alternative splicing products of the lamin A/C gene that is responsible for autosomal dominant Emery-Dreifuss muscular dystrophy (AD-EDMD). Aberrant expression patterns of nuclear lamins have been described in various types of cancer depending on the subtype of cancer, its aggressiveness, proliferative capacity and degree of differentiation. In general, the expression of A-type lamins (lamins A and C) has been correlated with a non-proliferating, differentiated state of cells and tissues. Lamins A and C, the products of the LMNA gene, are nuclear intermediate filament proteins and are the major structural components of the lamina network that underlies and supports the nuclear envelope.Synonyms: 70 kDa Lamin, LMN1, LMNA, Lamin A, Lamin A + C, Lamin-A/C, NY-REN-32, NYREN32, Nuclear Envelope Marker, Renal carcinoma antigen NY-REN-32|
|Pathways||Apoptosis, Caspase Cascade in Apoptosis, ER-Nucleus Signaling, Protein targeting to Nucleus|
Application DetailsProduct Details anti-Lamin A/C Antibody Target Details Lamin A/C Handling References for anti-Lamin A/C antibody (ABIN452378) Images back to top
Immunofluorescence Microscopy. Immunoblotting (Western). Working Dilution: Ready-to-use for Immunofluorescence microscopy. Incubation Time: 1 h at RT.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-Lamin A/C Antibody Target Details Lamin A/C Application Details References for anti-Lamin A/C antibody (ABIN452378) Images back to top
|Buffer||0.09 % Sodium Azide|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
Store the antibody undiluted at 2-8 °C.
Shelf life: one year from despatch.
|Expiry Date||12 months|
References for anti-Lamin A/C antibody (ABIN452378)Product Details anti-Lamin A/C Antibody Target Details Lamin A/C Application Details Handling Images back to top
Burke, Stewart: "Life at the edge: the nuclear envelope and human disease." in: Nature reviews. Molecular cell biology, Vol. 3, Issue 8, pp. 575-85, 2002
von Glasenapp, Benavente: "Fate of meiotic lamin C2 in rat spermatocytes cultured in the presence of okadaic acid." in: Chromosoma, Vol. 109, Issue 1-2, pp. 117-22, 2000
Talbot, Geiske, Knoll: "Oocyte pickup by the mammalian oviduct." in: Molecular biology of the cell, Vol. 10, Issue 1, pp. 5-8, 1999
Höger, Grund, Franke, Krohne: "Immunolocalization of lamins in the thick nuclear lamina of human synovial cells." in: European journal of cell biology, Vol. 54, Issue 1, pp. 150-6, 1991
Franke: "Nuclear lamins and cytoplasmic intermediate filament proteins: a growing multigene family." in: Cell, Vol. 48, Issue 1, pp. 3-4, 1987
SAUER, CHITTENDEN: "Deoxyribonucleic acid content of cell nuclei in the neural tube of the chick embryo: evidence for intermitotic migration of nuclei." in: Experimental cell research, Vol. 16, Issue 1, pp. 1-6, 1959