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anti-Pig (Porcine) Lamin B2 antibody for Flow Cytometry

Recommended Lamin B2 Antibody (supplied by: Log in to see )

Antigen
Lamin B2 (LMNB2) Antibodies
  • im:7142331
  • LAMB2
  • lamb2
  • lamin
  • lamin-b2
  • Lamin-L(II)
  • lmn2
  • LMN2
  • lmnb2
  • LMNB2
  • lmnb2-a
  • LOC100136040
  • RGD1563803
  • wu:fb94e05
  • wu:fb95e12
  • wu:fc15d06
  • wu:fc49h03
Reactivity
Hamster, Human, Mouse (Murine), Pig (Porcine), Xenopus laevis, Zebrafish (Danio rerio)
62
30
24
6
4
4
2
2
2
1
1
1
Host
Mouse
50
17
Clonality
Monoclonal
Conjugate
This Lamin B2 antibody is un-conjugated
1
1
1
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB)
55
15
12
11
11
11
8
8
5
4
2
1
1
1
Supplier
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Catalog No. ABIN360851
$ 390.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0010666461 ABIN2963278 IHC (fro) FACS WB Mouse IgG1 Log in to see LN43

Top referenced anti-Lamin B2 antibody for Flow Cytometry

Similar anti-Lamin B2 Antibodies

Application / Reactivity Mouse (Murine) Hamster Human Xenopus laevis Pig (Porcine) Zebrafish (Danio rerio)
Western Blotting (WB) 20 Antibodies 5 Antibodies 52 Antibodies 7 Antibodies 2 Antibodies 3 Antibodies
Immunoprecipitation (IP) 2 Antibodies 1 Antibodies 3 Antibodies 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 13 Antibodies 6 Antibodies
Immunohistochemistry (IHC) 16 Antibodies 6 Antibodies 2 Antibodies 2 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 4 Antibodies 3 Antibodies 8 Antibodies 3 Antibodies

General

Antigen Lamin B2 (LMNB2) Antibodies
Reactivity Hamster, Human, Mouse (Murine), Pig (Porcine), Xenopus laevis, Zebrafish (Danio rerio)
(62), (30), (24), (6), (4), (4), (2), (2), (2), (1), (1), (1)
Host Mouse
(50), (17)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Lamin B2 antibody is un-conjugated
(1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB)
(55), (15), (12), (11), (11), (11), (8), (8), (5), (4), (2), (1), (1), (1)
Pubmed 8 references available
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Product Details anti-Lamin B2 Antibody

Target Details Lamin B2 Application Details Handling References for anti-Lamin B2 Antibody (ABIN360851) Images
Specificity Reacts with an epitope located in the C-terminal part of Lamin B2.
Purification Purified
Immunogen Detergent insoluble fraction of potoroo cell line PtK1.
Clone LN43
Isotype IgG1

Target Details Lamin B2

Product Details anti-Lamin B2 Antibody Application Details Handling References for anti-Lamin B2 Antibody (ABIN360851) Images back to top
Antigen
Alternative Name Lamin-B2 (LMNB2) (LMNB2 Antibody Abstract)
Background An important part of the cell nucleus is formed by nuclear lamina. Nuclear lamins form a network of filaments at the nucleoplasmic site of the nuclear membrane. Two main subtypes of nuclear lamins can be distinguished, i.e. A-type lamins and B-type lamins. The A-type lamins comprise a set of three proteins arising from the same gene by alternative splicing, i.e. lamin A, lamin C and lamin Adel10, while the B-type lamins include two proteins arising from two distinct genes, i.e. lamin B1 and lamin B2. The nuclear lamins comprise a unique subclass of the intermediate filament protein family. They share a molecular domain organisation with the other intermediate filament proteins in that they are fibrous molecules that have an aminoterminal globular head, a central rod of a-helices and a carboxyterminal globular domain. Many biochemical and molecular features of lamins have been studied, but their functions remain still largely undetermined. One of the functions ascribed to the lamina is the maintenance of the structural integrity of the nucleus. Besides interactions with the nuclear membrane and other intermediate filaments, lamins interact with the nuclear chromatin. Eukaryotic chromatin is organised into loops, which are attached to the nuclear matrix. This organisation is thought to contribute to compaction of the chromatin and regulation of gene expression. Lamins, as part of the nuclear matrix, may be involved in these processes since chromatin binding sites have been detected in both A- and B-type lamins.Synonyms: LMN2, LMNB, LMNB-2, LMNB2, Lamin-B2, Nuclear Envelope Marker
Gene ID 84823
NCBI Accession NP_116126
UniProt Q03252
Research Area Cytoskeleton
Pathways Apoptosis, Caspase Cascade in Apoptosis

Application Details

Product Details anti-Lamin B2 Antibody Target Details Lamin B2 Handling References for anti-Lamin B2 Antibody (ABIN360851) Images back to top
Application Notes Suitable for Immunocytochemistry on Frozen Sections, Immunoblotting and FlowCytometry. Recommended Dilutions: 1/100-1/200 for Flow cytometry and for Immunohistochemistry with avidin-biotinylatedhorseradish peroxidase complex (ABC) as detection reagent.1/100-1/1000 for Immunoblotting applications.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-Lamin B2 Antibody Target Details Lamin B2 Application Details References for anti-Lamin B2 Antibody (ABIN360851) Images back to top
Concentration 1.0 mg/mL
Buffer PBS with 0.09 % Sodium Azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store lyophilized at 2 - 8 °C and reconstituted at -20 °C.

References for anti-Lamin B2 Antibody (ABIN360851)

Product Details anti-Lamin B2 Antibody Target Details Lamin B2 Application Details Handling Images back to top
Background publications

Machiels, Ramaekers, Kuijpers, Groenewoud, Oosterhuis, Looijenga: "Nuclear lamin expression in normal testis and testicular germ cell tumours of adolescents and adults." in: The Journal of pathology, Vol. 182, Issue 2, pp. 197-204, 1997

Broers, Machiels, Kuijpers, Smedts, van den Kieboom, Raymond, Ramaekers: "A- and B-type lamins are differentially expressed in normal human tissues." in: Histochemistry and cell biology, Vol. 107, Issue 6, pp. 505-17, 1997

Jansen, Machiels, Hopman, Broers, Bot, Arends, Ramaekers, Schouten: "Comparison of A and B-type lamin expression in reactive lymph nodes and nodular sclerosing Hodgkin's disease." in: Histopathology, Vol. 31, Issue 4, pp. 304-12, 1997

Machiels, Zorenc, Endert, Kuijpers, van Eys, Ramaekers, Broers: "An alternative splicing product of the lamin A/C gene lacks exon 10." in: The Journal of biological chemistry, Vol. 271, Issue 16, pp. 9249-53, 1996

Machiels, Broers, Raymond, de Ley, Kuijpers, Caberg, Ramaekers: "Abnormal A-type lamin organization in a human lung carcinoma cell line." in: European journal of cell biology, Vol. 67, Issue 4, pp. 328-35, 1996

Hozák, Sasseville, Raymond, Cook: "Lamin proteins form an internal nucleoskeleton as well as a peripheral lamina in human cells." in: Journal of cell science, Vol. 108 ( Pt 2), pp. 635-44, 1995

Jenkins, Hölman, Lyon, Lane, Stick, Hutchison: "Nuclei that lack a lamina accumulate karyophilic proteins and assemble a nuclear matrix." in: Journal of cell science, Vol. 106 ( Pt 1), pp. 275-85, 1994

Bridger, Kill, OFarrell, Hutchison: "Internal lamin structures within G1 nuclei of human dermal fibroblasts." in: Journal of cell science, Vol. 104 ( Pt 2), pp. 297-306, 1993