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|Application / Reactivity||Rat (Rattus)||Human|
|ELISA||6 Antibodies||12 Antibodies|
|Immunochromatography (IC)||1 Antibodies||2 Antibodies|
|Immunofluorescence (IF)||4 Antibodies||12 Antibodies|
|Immunofluorescence (fixed cells) (IF/ICC)||1 Antibodies||2 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||13 Antibodies||13 Antibodies|
|Immunohistochemistry (IHC)||6 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||3 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||6 Antibodies|
|Immunoprecipitation (IP)||1 Antibodies|
|Western Blotting (WB)||17 Antibodies|
|Enzyme Immunoassay (EIA)||1 Antibodies|
|Flow Cytometry (FACS)||13 Antibodies|
|Immunocytochemistry (ICC)||7 Antibodies|
|Antigen||Lamin B2 (LMNB2) Antibodies|
|Epitope||AA 580-597, C-Term Alternatives|
|Reactivity||Human, Rat (Rattus) Alternatives|
|Conjugate||This Lamin B2 antibody is un-conjugated Alternatives|
Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
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Product Details anti-Lamin B2 AntibodyTarget Details Lamin B2 Application Details Handling Images
Predicted Cross Reactivity: mouse
No cross reactivity with other proteins.
Predicted Cross Reactivity: Species predicted to be fit for the product based on sequence similarities.
Rabbit IgG polyclonal antibody for Lamin-B2(LMNB2) detection. Tested with WB, IHC-P, IHC-F in Human,Mouse,Rat.
Gene Name: lamin B2
Protein Name: Lamin-B2
|Purification||Immunogen affinity purified.|
|Immunogen||A synthetic peptide corresponding to a sequence at the C-terminus of human Lamin B2(580-597aa EEDLFHQQGDPRTTSRGC), identical to the related rat and mouse sequences.|
Target Details Lamin B2Product Details anti-Lamin B2 Antibody Application Details Handling Images back to top
|Alternative Name||LMNB2 (LMNB2 Antibody Abstract)|
|Background||Lamin B2, also called LMNB2, is a protein associated with laminopathies. The LMNB2 gene is mapped to the G-negative subtelomeric band p13.3 of chromosome 19 by in situ hybridization. The LMNB2 gene contains 12 exons. Model organisms have been used in the study of Lamin B2 function. A conditional knockout mouse line, called Lmnb2, is generated as part of the International Knockout Mouse Consortium program-a high-throughput mutagenesis project to generate and distribute animal models of disease to interested scientists. A highly sensitive procedure about mapping the start site of DNA replication in a 13.7-kb region of human chromosome 19 coding for lamin B2 is developed for the identification of the origin of bidirectional DNA synthesis in single-copy replicons of mammalian cells.|
|Pathways||Apoptosis, Caspase Cascade in Apoptosis|
Application DetailsProduct Details anti-Lamin B2 Antibody Target Details Lamin B2 Handling Images back to top
WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Predicted Species: Mouse, Rat
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
IHC-F: Concentration: 0.5-1 μg/mL, Tested Species: Rat, Predicted Species: Human, Mouse
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.
Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P) and IHC(F).
|Restrictions||For Research Use only|
HandlingProduct Details anti-Lamin B2 Antibody Target Details Lamin B2 Application Details Images back to top
|Reconstitution||Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.|
|Buffer||Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.|
|Preservative||Thimerosal (Merthiolate), Sodium azide|
|Precaution of Use||This product contains Thimerosal (Merthiolate),Sodium azide a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||-20 °C,4 °C|
At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.
|Expiry Date||12 months|
ImagesProduct Details anti-Lamin B2 Antibody Target Details Lamin B2 Application Details Handling back to top