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anti-Rat (Rattus) Lamin B2 antibody for Immunohistochemistry (Paraffin-embedded Sections)

Recommended Lamin B2 Antibody (supplied by: Log in to see )

Antigen
Lamin B2 (LMNB2) Antibodies
  • im:7142331
  • LAMB2
  • lamb2
  • lamin
  • lamin-b2
  • Lamin-L(II)
  • lmn2
  • LMN2
  • lmnb2
  • LMNB2
  • lmnb2-a
  • LOC100136040
  • RGD1563803
  • wu:fb94e05
  • wu:fb95e12
  • wu:fc15d06
  • wu:fc49h03
Epitope
AA 580-597, C-Term
13
3
3
3
2
2
2
2
1
1
1
1
Reactivity
Human, Rat (Rattus)
68
32
23
8
5
4
3
3
2
2
1
1
1
Host
Rabbit
52
21
Clonality
Polyclonal
Conjugate
This Lamin B2 antibody is un-conjugated
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
60
19
14
13
12
11
11
9
8
6
2
1
1
1
Supplier
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Catalog No. ABIN3044232
$ 200.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008913903 ABIN2489503 IHC (p) WB Rabbit IgG C-Term Log in to see Polyclonal
0.0008913903 ABIN1407356 IHC (p) WB HRP Rabbit IgG Log in to see Polyclonal
0.0008913903 ABIN1389463 IHC (p) WB Biotin Rabbit IgG Log in to see Polyclonal
0.0008913903 ABIN1385163 IF (p) IHC (p) WB Rabbit IgG Log in to see Polyclonal
0.0008913903 ABIN3031588 IHC (fro) IHC (p) WB Rabbit IgG AA 580-597 Log in to see Polyclonal

Similar anti-Lamin B2 Antibodies

Application / Reactivity Rat (Rattus) Human
ELISA 4 Antibodies 9 Antibodies
Immunochromatography (IC) 1 Antibodies 2 Antibodies
Immunofluorescence (IF) 3 Antibodies 9 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies 11 Antibodies
Immunohistochemistry (IHC) 4 Antibodies 20 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 2 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 6 Antibodies
Western Blotting (WB) 13 Antibodies
Enzyme Immunoassay (EIA) 1 Antibodies
Flow Cytometry (FACS) 12 Antibodies
Immunocytochemistry (ICC) 7 Antibodies
Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)) 1 Antibodies

General

Antigen Lamin B2 (LMNB2) Antibodies
Epitope AA 580-597, C-Term
(13), (3), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1)
Reactivity Human, Rat (Rattus)
(68), (32), (23), (8), (5), (4), (3), (3), (2), (2), (1), (1), (1)
Host Rabbit
(52), (21)
Clonality
Polyclonal
Conjugate This Lamin B2 antibody is un-conjugated
(1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(60), (19), (14), (13), (12), (11), (11), (9), (8), (6), (2), (1), (1), (1)
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Product Details anti-Lamin B2 Antibody

Target Details Lamin B2 Application Details Handling Images
Sequence EEDLFHQQGD PRTTSRGC
Cross-Reactivity (Details) Predicted Cross Reactivity: mouse
No cross reactivity with other proteins.
Predicted Cross Reactivity: Species predicted to be fit for the product based on sequence similarities.
Characteristics Rabbit IgG polyclonal antibody for Lamin-B2(LMNB2) detection. Tested with WB, IHC-P, IHC-F in Human,Mouse,Rat.
Gene Name: lamin B2
Protein Name: Lamin-B2
Purification Immunogen affinity purified.
Immunogen A synthetic peptide corresponding to a sequence at the C-terminus of human Lamin B2(580-597aa EEDLFHQQGDPRTTSRGC), identical to the related rat and mouse sequences.
Isotype IgG

Target Details Lamin B2

Product Details anti-Lamin B2 Antibody Application Details Handling Images back to top
Antigen
Alternative Name LMNB2 (LMNB2 Antibody Abstract)
Background Lamin B2, also called LMNB2, is a protein associated with laminopathies. The LMNB2 gene is mapped to the G-negative subtelomeric band p13.3 of chromosome 19 by in situ hybridization. The LMNB2 gene contains 12 exons. Model organisms have been used in the study of Lamin B2 function. A conditional knockout mouse line, called Lmnb2, is generated as part of the International Knockout Mouse Consortium program-a high-throughput mutagenesis project to generate and distribute animal models of disease to interested scientists. A highly sensitive procedure about mapping the start site of DNA replication in a 13.7-kb region of human chromosome 19 coding for lamin B2 is developed for the identification of the origin of bidirectional DNA synthesis in single-copy replicons of mammalian cells.
UniProt Q03252
Pathways Apoptosis, Caspase Cascade in Apoptosis

Application Details

Product Details anti-Lamin B2 Antibody Target Details Lamin B2 Handling Images back to top
Application Notes WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Predicted Species: Mouse, Rat
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
IHC-F: Concentration: 0.5-1 μg/mL, Tested Species: Rat, Predicted Species: Human, Mouse
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.
Comment

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P) and IHC(F).

Restrictions For Research Use only

Handling

Product Details anti-Lamin B2 Antibody Target Details Lamin B2 Application Details Images back to top
Format Lyophilized
Reconstitution Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
Concentration 500 μg/mL
Buffer Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.
Preservative Thimerosal (Merthiolate), Sodium azide
Precaution of Use This product contains Thimerosal (Merthiolate),Sodium azide a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C,4 °C
Storage Comment At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.
Expiry Date 12 months

Images

Product Details anti-Lamin B2 Antibody Target Details Lamin B2 Application Details Handling back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-Lamin B2 antibody (LMNB2) (AA 580-597) (ABIN3044232) Anti-Lamin B2 antibody, IHC(P) IHC(P): Rat Intestine Tissue
Western Blotting (WB) image for anti-Lamin B2 antibody (LMNB2) (AA 580-597) (ABIN3044232) Anti-Lamin B2 antibody, Western blotting Lane 1: HELA Cell Lysate Lane 2: U87 Cell Ly...