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anti-Human NME1 antibody for Western Blotting

Recommended NME1 Antibody (supplied by: Log in to see )

Antigen
Non-Metastatic Cells 1, Protein (NM23A) Expressed in (NME1) Antibodies
  • AL024257
  • AWD
  • CHH
  • GAAD
  • NB
  • NBR-A
  • NBR-B
  • NBS
  • NDKA
  • NDK I
  • NDPK-A
  • ndpk-b
  • NDPK-Z1
  • NDPKA
  • ndpkb
  • NDPK I
  • ndpkz1
  • NM23
  • NM23-B
  • NM23-C1
  • NM23-H1
  • nm23-h2
  • NM23-M1
  • NM23-Z1
  • NM23A
  • nm23b
  • nm23ndk
  • nm23ndk-a
  • nme1
  • NME1
  • NME1-1
  • NME1-NME2
  • nme2
  • nme2b1
  • puf
  • RMRPR
  • RRP2
  • T30A10.80
  • T30A10_80
Epitope
C-Term, AA 102-131
26
21
14
13
12
12
11
7
5
5
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
Reactivity
Human
272
39
37
12
6
6
3
3
2
1
1
Host
Rabbit
140
132
2
2
2
Clonality
Polyclonal
Conjugate
This NME1 antibody is un-conjugated
12
11
8
7
6
6
Application
Enzyme Immunoassay (EIA), Western Blotting (WB)
217
117
77
57
37
12
12
10
9
6
2
2
2
1
Supplier
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Catalog No. ABIN953623
$ 401.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.13062482 ABIN953624 EIA WB Rabbit Ig AA 28-57, Middle Region Log in to see Polyclonal 3
0.13062482 ABIN2792090 DB IHC WB Rabbit N-Term Log in to see Polyclonal 1
0.13062482 ABIN392655 IF IHC (p) WB Rabbit Ig AA 25-54, N-Term Log in to see Polyclonal 2
0.13062482 ABIN969317 ICC FACS IHC ELISA WB Mouse IgG1 Log in to see 4B2 3
0.13062482 ABIN967854 IF IP IHC WB Mouse IgG2b AA 44-152 Log in to see 56-Nm23 3
0.13062482 ABIN967855 IF WB Mouse IgG2b AA 44-152 Log in to see 56 3
0.13062482 ABIN658858 WB Rabbit Ig AA 103-131, C-Term Log in to see Polyclonal 3
0.13062482 ABIN1882270 WB Mouse IgG2a Log in to see 1172CT2-4-1-1 6
0.13062482 ABIN782418 EIA IF IHC (p) IP WB Mouse IgG1 AA 43-153 Log in to see 1D7
0.13062482 ABIN1108395 EIA FACS IF IHC (p) WB Mouse IgG1 Log in to see 4B2 1
0.13062482 ABIN562004 IF IHC (p) IP ELISA WB Mouse IgG1 kappa AA 43-152, partial Log in to see 1D7 1
0.13062482 ABIN1533358 ELISA WB Rabbit IgG Log in to see Polyclonal
0.13062482 ABIN3043572 WB Rabbit IgG AA 26-58, N-Term Log in to see Polyclonal
0.13062482 ABIN498563 IHC (p) WB Rabbit Log in to see Polyclonal
0.13062482 ABIN2780437 DB WB Rabbit C-Term Log in to see Polyclonal
0.13062482 ABIN3044197 ICC IHC (p) WB Rabbit IgG AA 137-152, C-Term Log in to see Polyclonal
0.13062482 ABIN2706696 IHC WB Rabbit Center Log in to see Polyclonal
0.13062482 ABIN1873911 IHC WB Rabbit IgG C-Term Log in to see Polyclonal
0.13062482 ABIN1538234 WB Rabbit Ig AA 29-57, Center Log in to see Polyclonal
0.13062482 ABIN393634 IF WB Mouse IgG1 kappa AA 1-153 Log in to see 2H1

Top referenced anti-NME1 antibody for Western Blotting

Similar anti-NME1 Antibodies

Application / Reactivity Human
Dot Blot (DB) 3 Antibodies
ELISA 114 Antibodies
Enzyme Immunoassay (EIA) 7 Antibodies
Flow Cytometry (FACS) 9 Antibodies
Immunochromatography (IC) 2 Antibodies
Immunocytochemistry (ICC) 12 Antibodies
Immunofluorescence (IF) 38 Antibodies
Immunohistochemistry (IHC) 79 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 58 Antibodies
Immunoprecipitation (IP) 12 Antibodies
Luminex Assay (LMNX) 10 Antibodies
RNA Interference (RNAi) 1 Antibodies
Western Blotting (WB) 216 Antibodies

General

Antigen Non-Metastatic Cells 1, Protein (NM23A) Expressed in (NME1) Antibodies
Epitope C-Term, AA 102-131
(26), (21), (14), (13), (12), (12), (11), (7), (5), (5), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Reactivity Human
(272), (39), (37), (12), (6), (6), (3), (3), (2), (1), (1)
Host Rabbit
(140), (132), (2), (2), (2)
Clonality
Polyclonal
Conjugate This NME1 antibody is un-conjugated
(12), (11), (8), (7), (6), (6)
Application Enzyme Immunoassay (EIA), Western Blotting (WB)
(217), (117), (77), (57), (37), (12), (12), (10), (9), (6), (2), (2), (2), (1)
Pubmed 3 references available
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Product Details anti-NME1 Antibody

Target Details NME1 Application Details Handling References for anti-NME1 Antibody (ABIN953623) Images
Specificity This antibody recognizes NDP kinase A (NME1) at C-term. Other speci9es not tested.
Cross-Reactivity (Details) Species reactivity (expected):Rat.
Species reactivity (tested):Human.
Purification Purified through a Protein A column followed by peptide affinity purification
Immunogen Synthetic peptide - KLH conjugated - corresponding to the C-terminal region (between 102-131aa) of human NDP kinase A
Isotype Ig

Target Details NME1

Product Details anti-NME1 Antibody Application Details Handling References for anti-NME1 Antibody (ABIN953623) Images back to top
Antigen
Alternative Name NDP Kinase A (NME1 Antibody Abstract)
Background The NME1 gene was identified because of its reduced mRNA transcript levels in highly metastatic cells. Nucleoside diphosphate kinase (NDK) exists as a hexamer composed of 'A' (encoded by this gene) and 'B' (encoded by NME2) isoforms. Mutations in this gene have been identified in aggressive neuroblastomas. Two transcript variants encoding different isoforms have been found for this gene. Co-transcription of this gene and the neighboring downstream gene (NME2) generates naturally-occurring transcripts (NME1-NME2), which encodes a fusion protein comprised of sequence sharing identity with each individual gene product. Function: Major role in the synthesis of nucleoside triphosphates other than ATP. Possesses nucleoside-diphosphate kinase, serine/threonine-specific protein kinase, geranyl and farnesyl pyrophosphate kinase, histidine protein kinase and 3'-5' exonuclease activities. Involved in cell proliferation, differentiation and development, signal transduction, G protein- coupled receptor endocytosis, and gene expression. Required for neural development including neural patterning and cell fate determination. Cellular Location: Cytopla sm. Nucleus. Note=Cell-cycle dependent nuclear localization which can be induced by interaction with Epstein-barr viral proteins or by degradation of the SET complex by GzmA. Tissue Location: Isoform 1 is expressed in heart, brain, placenta, lung, liver, skeletal muscle, pancreas, spleen and thymus. Expressed in lung carcinoma cell lines but not in normal lung tissues. Isoform 2 is ubiquitously expressed and its expression is also related to tumor differentiation. Isoform 3 is ubiquitously expressed.Synonyms: GAAD, Granzyme A-activated DNase, NDPKA, NM23, NME1, Nucleoside diphosphate kinase A, nm23-H1
Molecular Weight 17149 Da
Gene ID 4830
NCBI Accession NP_000260
Research Area Kinases/Phosphatases, Cancer, Transcription Factors
Pathways Apoptosis

Application Details

Product Details anti-NME1 Antibody Target Details NME1 Handling References for anti-NME1 Antibody (ABIN953623) Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-NME1 Antibody Target Details NME1 Application Details References for anti-NME1 Antibody (ABIN953623) Images back to top
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS, 0.09 % (W/V) Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

References for anti-NME1 Antibody (ABIN953623)

Product Details anti-NME1 Antibody Target Details NME1 Application Details Handling Images back to top
Background publications

Boissan, De Wever, Lizarraga, Wendum, Poincloux, Chignard, Desbois-Mouthon, Dufour, Nawrocki-Raby, Birembaut, Bracke, Chavrier, Gespach, Lacombe: "Implication of metastasis suppressor NM23-H1 in maintaining adherens junctions and limiting the invasive potential of human cancer cells." in: Cancer research, Vol. 70, Issue 19, pp. 7710-22, 2010

Wang, Yi, Tsai, Tee, Ko, Han, Liu, Lin, Yang: "Significant association of genetic polymorphism of human nonmetastatic clone 23 type 1 gene with an increased risk of endometrial cancer." in: Gynecologic oncology, Vol. 119, Issue 1, pp. 70-5, 2010

Conery, Sever, Harlow: "Nucleoside diphosphate kinase Nm23-H1 regulates chromosomal stability by activating the GTPase dynamin during cytokinesis." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 107, Issue 35, pp. 15461-6, 2010

Images

Product Details anti-NME1 Antibody Target Details NME1 Application Details Handling References for anti-NME1 Antibody (ABIN953623) back to top
Supplier Images
Western Blotting (WB) image for anti-NME1 antibody (Non-Metastatic Cells 1, Protein (NM23A) Expressed in) (C-Term) (ABIN953623) Western blot analysis of NDP kinase A Antibody (C-term) Cat.-No AP52898PU-N in Hela, ...