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|Application / Reactivity||Human||Mouse (Murine)||Rat (Rattus)|
|Immunofluorescence (fixed cells) (IF/ICC)||5 Antibodies||2 Antibodies||1 Antibodies|
|Western Blotting (WB)||233 Antibodies||51 Antibodies||39 Antibodies|
|RNA Interference (RNAi)||1 Antibodies|
|Luminex Assay (LMNX)||10 Antibodies|
|Immunoprecipitation (IP)||13 Antibodies||3 Antibodies||2 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||61 Antibodies||9 Antibodies||17 Antibodies|
|Immunohistochemistry (IHC)||85 Antibodies||19 Antibodies||18 Antibodies|
|Antigen||Non-Metastatic Cells 1, Protein (NM23A) Expressed in (NME1) Antibodies|
|Reactivity||Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This NME1 antibody is un-conjugated Alternatives|
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
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Product Details anti-NME1 AntibodyTarget Details NME1 Application Details Handling Images
|Specificity||This antibody detects endogenous levels of NM23-H1 protein. (region surrounding Tyr52)|
Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.
|Purity||> 95 % by SDS-PAGE|
Target Details NME1Product Details anti-NME1 Antibody Application Details Handling Images back to top
|Alternative Name||NDP Kinase A (NME1 Antibody Abstract)|
|Background||The nm23 gene, a potential suppressor of metastasis, was originally identified by differential hybridization between two murine melanoma sub-lines, one with a high and the second with a low metastatic capacity. Highly metastatic sublines exhibit much lower levels of nm23 than less metastatic cells. Based on sequence analysis, nm23 appears highly related to nucleotide diphosphate kinases (NDP). In humans, NDP kinases A and B are identical to two isotypes of human nm23 homologs, namely nm23-H1 and -H2, respectively. nm23-H2 is identical in sequence to PuF, a transcription factor that binds to nucleasehypersensitive elements at positions 142 to 115 of the human c-Myc promotor.Synonyms: GAAD, Granzyme A-activated DNase, NDPKA, NM23, NME1, Nucleoside diphosphate kinase A, nm23-H1|
|Molecular Weight||approx. 20 kDa|
|Research Area||Kinases/Phosphatases, Cancer, Transcription Factors|
|Pathways||Apoptosis, Nucleotide Phosphorylation, Carbohydrate Homeostasis, Ribonucleoside Biosynthetic Process|
Application DetailsProduct Details anti-NME1 Antibody Target Details NME1 Handling Images back to top
Western Blot: 1/500 - 1/1000. Immunohistochemistry: 1/50 - 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-NME1 Antibody Target Details NME1 Application Details Images back to top
|Buffer||Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.|
ImagesProduct Details anti-NME1 Antibody Target Details NME1 Application Details Handling back to top