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anti-Rat (Rattus) NME1 antibody for Immunocytochemistry

Recommended NME1 Antibody (supplied by: Log in to see )

Antigen
Non-Metastatic Cells 1, Protein (NM23A) Expressed in (NME1) Antibodies
  • AL024257
  • AWD
  • CHH
  • GAAD
  • NB
  • NBR-A
  • NBR-B
  • NBS
  • NDKA
  • NDK I
  • NDPK-A
  • ndpk-b
  • NDPK-Z1
  • NDPKA
  • ndpkb
  • NDPK I
  • ndpkz1
  • NM23
  • NM23-B
  • NM23-C1
  • NM23-H1
  • nm23-h2
  • NM23-M1
  • NM23-Z1
  • NM23A
  • nm23b
  • nm23ndk
  • nm23ndk-a
  • nme1
  • NME1
  • NME1-1
  • NME1-NME2
  • nme2
  • nme2b1
  • puf
  • RMRPR
  • RRP2
  • T30A10.80
  • T30A10_80
Epitope
AA 137-152, C-Term
26
21
14
13
13
12
11
7
5
5
3
3
3
3
2
2
2
2
2
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
272
38
36
12
6
6
3
3
2
1
1
Host
Rabbit
140
132
2
2
2
Clonality
Polyclonal
Conjugate
This NME1 antibody is un-conjugated
12
11
8
7
6
6
Application
Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
217
117
77
56
37
12
11
10
9
7
2
2
2
1
Supplier
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Catalog No. ABIN3044197
$ 200.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0010194078 ABIN2988568 ICC IF IHC WB Rabbit IgG C-Term Log in to see Polyclonal
0.0010194078 ABIN3032024 ICC IHC (p) WB Rabbit IgG C-Term Log in to see Polyclonal

Similar anti-NME1 Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine)
Dot Blot (DB) 1 Antibodies 2 Antibodies
ELISA 11 Antibodies 14 Antibodies
Immunocytochemistry (ICC) 3 Antibodies 5 Antibodies
Immunofluorescence (IF) 3 Antibodies 4 Antibodies
Immunohistochemistry (IHC) 14 Antibodies 14 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 11 Antibodies 4 Antibodies
Immunoprecipitation (IP) 2 Antibodies 2 Antibodies
Western Blotting (WB) 28 Antibodies
Enzyme Immunoassay (EIA) 1 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 1 Antibodies

General

Antigen Non-Metastatic Cells 1, Protein (NM23A) Expressed in (NME1) Antibodies
Epitope AA 137-152, C-Term
(26), (21), (14), (13), (13), (12), (11), (7), (5), (5), (3), (3), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(272), (38), (36), (12), (6), (6), (3), (3), (2), (1), (1)
Host Rabbit
(140), (132), (2), (2), (2)
Clonality
Polyclonal
Conjugate This NME1 antibody is un-conjugated
(12), (11), (8), (7), (6), (6)
Application Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(217), (117), (77), (56), (37), (12), (11), (10), (9), (7), (2), (2), (2), (1)
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Product Details anti-NME1 Antibody

Target Details NME1 Application Details Handling Images
Sequence EELVDYTSCA QNWIYE
Cross-Reactivity (Details) No cross reactivity with other proteins.
Characteristics Rabbit IgG polyclonal antibody for Nucleoside diphosphate kinase A(NME1) detection. Tested with WB, IHC-P, ICC in Human,Mouse,Rat.
Gene Name: NME/NM23 nucleoside diphosphate kinase 1
Protein Name: Nucleoside diphosphate kinase A(NDK A)
Purification Immunogen affinity purified.
Immunogen A synthetic peptide corresponding to a sequence at the C-terminus of human NM23A(137-152aa EELVDYTSCAQNWIYE), different from the related mouse sequence by two amino acids and from rat sequence by one amino acid.
Isotype IgG

Target Details NME1

Product Details anti-NME1 Antibody Application Details Handling Images back to top
Antigen
Alternative Name NME1 (NME1 Antibody Abstract)
Background NME1(NME/NM23 nucleoside diphosphate kinase 1) also called non-metastatic cells 1, protein(NM23A) expressed in,NM23, NM23-H1, NDPKA, GAAD or AWD, is an enzyme that in humans is encoded by the NME1 gene. The promoters of the mouse and human NME1 genes, like those of other NME genes, contain several binding sites for AP2, NF1, Sp1, LEF1, and response elements to glucocorticoid receptors. The NME1 gene is mapped on 17q21.33. Immunofluorescence microscopy demonstrated colocalization of NME1 in nuclei of B cells expressing EBNA3C. Expression of EBNA3C reversed the ability of NME1 to inhibit migration of BL and breast carcinoma cells. NM23H1 bound SET and was released from inhibition by GZMA cleavage of SET. After GZMA loading or cytotoxic T lymphocyte attack, SET and NM23H1 translocated to the nucleus and SET was degraded, allowing NM23H1 to nick chromosomal DNA. Using a Drosophila model system, Dammai et al.(2003) showed that the Drosophila NME1 homolog, awd, regulates trachea cell motility by modulating FGFR levels through a dynamin -mediated pathway.
UniProt P15531
Pathways Apoptosis

Application Details

Product Details anti-NME1 Antibody Target Details NME1 Handling Images back to top
Application Notes WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Mouse, Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
ICC: Concentration: 0.5-1 μg/mL, Tested Species: Human
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.
Comment

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P) and ICC.

Restrictions For Research Use only

Handling

Product Details anti-NME1 Antibody Target Details NME1 Application Details Images back to top
Format Lyophilized
Reconstitution Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
Concentration 500 μg/mL
Buffer Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.
Preservative Thimerosal (Merthiolate), Sodium azide
Precaution of Use This product contains Thimerosal (Merthiolate),Sodium azide a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C,4 °C
Storage Comment At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.
Expiry Date 12 months

Images

Product Details anti-NME1 Antibody Target Details NME1 Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-NME1 antibody (Non-Metastatic Cells 1, Protein (NM23A) Expressed in) (AA 137-152) (ABIN3044197) anti-Non-Metastatic Cells 1, Protein (NM23A) Expressed in (NME1) (AA 137-152), (C-Term) antibody
Immunohistochemistry (IHC) image for anti-NME1 antibody (Non-Metastatic Cells 1, Protein (NM23A) Expressed in) (AA 137-152) (ABIN3044197) Anti-NM23A antibody, ICC ICC: HELA Cell
Immunohistochemistry (IHC) image for anti-NME1 antibody (Non-Metastatic Cells 1, Protein (NM23A) Expressed in) (AA 137-152) (ABIN3044197) Anti-NM23A antibody, IHC(P) IHC(P): Rat Cerebellum Tissue