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anti-Cow (Bovine) Perforin 1 antibody for Immunofluorescence

Recommended Perforin 1 Antibody (supplied by: Log in to see )

Antigen
Perforin 1 (Pore Forming Protein) (PRF1) Antibodies
  • Cyta
  • FLH2
  • HPLH2
  • LOC443187
  • P1
  • perforin
  • Pfn
  • PFN1
  • Pfp
  • PFP
  • Prf-1
  • prf1
  • PRF1
  • RATCYTA
Reactivity
Cow (Bovine), Human
162
48
21
18
1
1
Host
Mouse
108
72
28
Clonality
Monoclonal
Conjugate
This Perforin 1 antibody is un-conjugated
18
13
10
5
4
4
4
3
3
2
2
2
2
2
2
2
2
2
2
1
1
1
Application
Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (IHC)
92
79
62
59
45
44
44
42
31
9
6
6
3
2
2
1
1
1
1
1
Supplier
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Available images

Catalog No. ABIN262792
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0010843406 ABIN4344819 FACS ICC IF IHC IHC (fro) IHC (p) IP DyLight 350 Mouse IgG2b kappa Log in to see dG9
0.0010843406 ABIN4344821 FACS ICC IF IHC IHC (fro) IHC (p) IP DyLight 405 Mouse IgG2b kappa Log in to see dG9
0.0010843406 ABIN4344823 FACS ICC IF IHC IHC (fro) IHC (p) IP PE Mouse IgG2b kappa Log in to see dG9
0.0010843406 ABIN4344825 FACS ICC IF IHC IHC (fro) IHC (p) IP PerCP Mouse IgG2b kappa Log in to see dG9
0.0010843406 ABIN4344827 FACS ICC IF IHC IHC (fro) IHC (p) IP Allophycocyanin Mouse IgG2b kappa Log in to see dG9
0.0010843406 ABIN4344829 FACS ICC IF IHC IHC (fro) IHC (p) IP DyLight 755 Mouse IgG2b kappa Log in to see dG9
0.0010843406 ABIN4344831 FACS ICC IF IHC IHC (fro) IHC (p) IP DyLight 488 Mouse IgG2b kappa Log in to see dG9
0.0010843406 ABIN4344833 FACS ICC IF IHC IHC (fro) IHC (p) IP DyLight 405LS Mouse IgG2b kappa Log in to see dG9
0.0010843406 ABIN4344835 FACS ICC IF IHC IHC (fro) IHC (p) IP DyLight 680 Mouse IgG2b kappa Log in to see dG9
0.0010843406 ABIN4344837 FACS ICC IF IHC IHC (fro) IHC (p) IP Alexa Fluor 405 Mouse IgG2b kappa Log in to see dG9
0.0010843406 ABIN4344839 FACS ICC IF IHC IHC (fro) IHC (p) IP Alexa Fluor 488 Mouse IgG2b kappa Log in to see dG9
0.0010843406 ABIN4344841 FACS ICC IF IHC IHC (fro) IHC (p) IP Alexa Fluor 647 Mouse IgG2b kappa Log in to see dG9
0.0010843406 ABIN4344843 FACS ICC IF IHC IHC (fro) IHC (p) IP Alexa Fluor 700 Mouse IgG2b kappa Log in to see dG9
0.0010843406 ABIN2689879 IHC (f) IHC (fro) IF ICS IP WB Mouse IgG2b, kappa Log in to see deltaG9
0.0010843406 ABIN3039418 ICC FACS IF IP IHC Mouse IgG2b Log in to see
0.0010843406 ABIN3039419 ICC FACS IF IP IHC PE Mouse IgG2b Log in to see

Similar anti-Perforin 1 Antibodies

Application / Reactivity Human
ELISA 23 Antibodies
ELISA (Capture) 2 Antibodies
ELISA (Detection) 2 Antibodies
ELISpot 6 Antibodies
ELISpot (Capture) (ELISPOT (Capture)) 1 Antibodies
ELISpot (Detection) (ELISPOT (Detection)) 1 Antibodies
Enzyme Immunoassay (EIA) 1 Antibodies
Flow Cytometry (FACS) 77 Antibodies
Functional Studies (Func) 1 Antibodies
Immunochromatography (IC) 3 Antibodies
Immunocytochemistry (ICC) 24 Antibodies
Immunofluorescence (IF) 30 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 1 Antibodies
Immunohistochemistry (IHC) 45 Antibodies
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) 3 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 27 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 27 Antibodies

General

Antigen Perforin 1 (Pore Forming Protein) (PRF1) Antibodies
Reactivity Cow (Bovine), Human
(162), (48), (21), (18), (1), (1)
Host Mouse
(108), (72), (28)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Perforin 1 antibody is un-conjugated
(18), (13), (10), (5), (4), (4), (4), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1)
Application Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (IHC)
(92), (79), (62), (59), (45), (44), (44), (42), (31), (9), (6), (6), (3), (2), (2), (1), (1), (1), (1), (1)
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Product Details anti-Perforin 1 Antibody

Target Details Perforin 1 Application Details Handling Images
Specificity Perforin (dG9)
No Cross-Reactivity Mouse (Murine)
Purification affinity purified
Immunogen Purified granules from the human lymphoma cell line YT.
Clone DG9
Isotype IgG2b kappa

Target Details Perforin 1

Product Details anti-Perforin 1 Antibody Application Details Handling Images back to top
Antigen
Alternative Name Perforin (PRF1 Antibody Abstract)
Background Perforin is a 70 kD cytolytic protein that is expressed in the cytoplasmic granules ofcytotoxic T lymphocytes (CTLs) and natural killer (NK) cells. Perforin is one of the majoreffector molecules used by cytotoxic T cells and NK cells to mediate targeted cell lysis.The dG9 antibody recognizes human and bovine perforin and has been shown to beuseful for flow cytometry, immunoprecipitation, immunohistochemistry, andimmunofluorescence staining.
Gene Symbol: PRF1
Gene ID 5551
Pathways Apoptosis, Caspase Cascade in Apoptosis

Application Details

Product Details anti-Perforin 1 Antibody Target Details Perforin 1 Handling Images back to top
Application Notes Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is: 0.5 microgram per million cells in 100 microliter or 100 microliter of whole blood. Immunohistochemistry-frozen/paraffin. It is recommended that the reagent be titrated for optimal performance for each application.
Recommended dilutions: Immunoprecipitation 1:10-1:500, Immunohistochemistry-Paraffin 1:10-1:500, Immunohistochemistry-Frozen 1:10-1:500, Flow Cytometry < 0.06 µg/10^6 cells, Immunocytochemistry/Immunofluorescence 1:10-1:500, Immunohistochemistry 1:10-1:500
Restrictions For Research Use only

Handling

Product Details anti-Perforin 1 Antibody Target Details Perforin 1 Application Details Images back to top
Concentration 0.5 mg/mL
Buffer PBS [pH 7.2], Sodium Azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Do not freeze.
Storage 4 °C

Images

Product Details anti-Perforin 1 Antibody Target Details Perforin 1 Application Details Handling back to top
Supplier Images
Flow Cytometry (FACS) image for anti-Perforin 1 antibody (Perforin 1 (Pore Forming Protein)) (ABIN262792) Whole blood lymphocytes stained intracellularly with dG9 FITC