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anti-Human Perforin 1 antibody for Intracellular Staining

Recommended Perforin 1 Antibody (supplied by: Log in to see )

Antigen
Perforin 1 (Pore Forming Protein) (PRF1) Antibodies
  • Cyta
  • FLH2
  • HPLH2
  • LOC443187
  • P1
  • perforin
  • Pfn
  • PFN1
  • Pfp
  • PFP
  • Prf-1
  • prf1
  • PRF1
  • RATCYTA
Reactivity
Human
143
32
17
8
1
1
Host
Mouse
91
70
14
Clonality
Monoclonal
Conjugate
This Perforin 1 antibody is conjugated to FITC
17
11
10
4
4
3
3
2
2
1
1
1
1
Application
Flow Cytometry (FACS), Intracellular Staining (ICS), Immunohistochemistry (IHC)
74
63
33
31
30
17
17
16
14
8
6
6
3
2
2
1
1
1
1
1
Supplier
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Catalog No. ABIN2680802
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.000871331 ABIN2680808 FACS ICS PE-Cy7 Mouse IgG2b kappa Log in to see dG9 (delta G9) 1
0.000871331 ABIN2680806 FACS ICS APC Mouse IgG2b kappa Log in to see dG9 (delta G9) 3
0.000871331 ABIN2680810 FACS ICS PerCP-eFluor® 710 Mouse IgG2b kappa Log in to see dG9 (delta G9) 3
0.000871331 ABIN2680859 FACS ICS PE Mouse IgG2b kappa Log in to see dG9 (delta G9) 3
0.000871331 ABIN2689879 IHC (f) IHC (fro) IF ICS IP WB Mouse IgG2b, kappa Log in to see deltaG9

Top referenced anti-Perforin 1 antibody for Intracellular Staining

Similar anti-Perforin 1 Antibodies

Application / Reactivity Human
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ELISA (Capture) 2 Antibodies
ELISA (Detection) 2 Antibodies
ELISpot 6 Antibodies
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ELISpot (Detection) (ELISPOT (Detection)) 1 Antibodies
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Flow Cytometry (FACS) 63 Antibodies
Functional Studies (Func) 1 Antibodies
Immunochromatography (IC) 3 Antibodies
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Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 1 Antibodies
Immunohistochemistry (IHC) 31 Antibodies
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) 3 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 14 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 13 Antibodies

General

Antigen Perforin 1 (Pore Forming Protein) (PRF1) Antibodies
Reactivity Human
(143), (32), (17), (8), (1), (1)
Host Mouse
(91), (70), (14)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Perforin 1 antibody is conjugated to FITC
(17), (11), (10), (4), (4), (3), (3), (2), (2), (1), (1), (1), (1)
Application Flow Cytometry (FACS), Intracellular Staining (ICS), Immunohistochemistry (IHC)
(74), (63), (33), (31), (30), (17), (17), (16), (14), (8), (6), (6), (3), (2), (2), (1), (1), (1), (1), (1)
Pubmed 3 references available
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Product Details anti-Perforin 1 Antibody

Target Details Perforin 1 Application Details Handling References for anti-Perforin 1 Antibody (ABIN2680802) Images
Specificity The dG9 antibody clone reacts with human perforin (pore-forming protein, pfp). Perforin is one of the cytolytic mediators present in the cytoplasmic granules of cytotoxic T lymphocytes (CTL) and natural killer cells (NK). Perforin is involved in the killing function by CTLs and NKs and has an important role in the immune response against tumors and virus infections.
Characteristics The dG9 antibody clone reacts with human perforin (pore-forming protein, pfp). Perforin is one of the cytolytic mediators present in the cytoplasmic granules of cytotoxic T lymphocytes (CTL) and natural killer cells (NK). Perforin is involved in the killing function by CTLs and NKs and has an important role in the immune response against tumors and virus infections.
Clone DG9 (delta G9)
Isotype IgG2b kappa

Target Details Perforin 1

Product Details anti-Perforin 1 Antibody Application Details Handling References for anti-Perforin 1 Antibody (ABIN2680802) Images back to top
Antigen
Alternative Name Perforin (PRF1 Antibody Abstract)
Research Area Immunology, Innate Immunity, Adaptive Immunity, Inflammation
Pathways Apoptosis, Caspase Cascade in Apoptosis

Application Details

Product Details anti-Perforin 1 Antibody Target Details Perforin 1 Handling References for anti-Perforin 1 Antibody (ABIN2680802) Images back to top
Application Notes Applications reported: This dG9 (delta G9) antibody has been reported for use in intracellular staining followed by flow cytometric analysis, and immunohistochemical staining.

Applications tested: This dG9 (delta G9) antibody has been tested by intracellular flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 μL (0.125 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 105 to 108 cells/test.
Restrictions For Research Use only

Handling

Product Details anti-Perforin 1 Antibody Target Details Perforin 1 Application Details References for anti-Perforin 1 Antibody (ABIN2680802) Images back to top
Buffer aqueous buffer, 0.09 % sodium azide, may contain carrier protein/stabilizer
Handling Advice Do not freeze. Light sensitive material.
Storage 4 °C
Storage Comment Store at 2-8°C.

References for anti-Perforin 1 Antibody (ABIN2680802)

Product Details anti-Perforin 1 Antibody Target Details Perforin 1 Application Details Handling Images back to top
Background publications

Liu, Walsh, Young: "Perforin: structure and function." in: Immunology today, Vol. 16, Issue 4, pp. 194-201, 1995

Hameed, Olsen, Cheng, Fox, Hruban, Podack: "Immunohistochemical identification of cytotoxic lymphocytes using human perforin monoclonal antibody." in: The American journal of pathology, Vol. 140, Issue 5, pp. 1025-30, 1992

Smyth, Ortaldo, Shinkai, Yagita, Nakata, Okumura, Young: "Interleukin 2 induction of pore-forming protein gene expression in human peripheral blood CD8+ T cells." in: The Journal of experimental medicine, Vol. 171, Issue 4, pp. 1269-81, 1990

Images

Product Details anti-Perforin 1 Antibody Target Details Perforin 1 Application Details Handling References for anti-Perforin 1 Antibody (ABIN2680802) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-Perforin 1 antibody (Perforin 1 (Pore Forming Protein))  (FITC) (ABIN2680802) Intracellular staining of normal human peripheral blood cells with Anti-Human CD8 APC...