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anti-Human Perforin 1 antibody for Immunofluorescence

Recommended Perforin 1 Antibody (supplied by: Log in to see )

Antigen
Perforin 1 (Pore Forming Protein) (PRF1) Antibodies
  • Cyta
  • FLH2
  • HPLH2
  • LOC443187
  • P1
  • perforin
  • Pfn
  • PFN1
  • Pfp
  • PFP
  • Prf-1
  • prf1
  • PRF1
  • RATCYTA
Reactivity
Human, Mouse (Murine)
161
47
21
18
1
1
Host
Rabbit
108
71
28
Clonality
Polyclonal
Conjugate
This Perforin 1 antibody is un-conjugated
18
13
10
5
4
4
4
3
3
2
2
2
2
2
2
2
2
2
2
1
1
1
Application
Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)
91
79
61
59
44
44
44
42
31
9
6
6
3
2
2
1
1
1
1
1
Supplier
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Catalog No. ABIN2403408
$ 588.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0010843406 ABIN411557 IF IP IHC WB Rabbit IgG Log in to see Polyclonal
0.0010843406 ABIN222977 FACS IF IP IHC Mouse IgG2b Log in to see deltaG9 1
0.0010843406 ABIN126932 FACS IHC (fro) IF IP Mouse IgG2b Log in to see dG9
0.0010843406 ABIN262793 ICC FACS IF FITC Mouse IgG2b kappa Log in to see dG9
0.0010843406 ABIN262792 ICC FACS IHC (fro) IF IHC (p) IP IHC Mouse IgG2b kappa Log in to see dG9
0.0010843406 ABIN187239 EIA FACS IHC (fro) IF WB Rat IgG2a AA 189-360 Log in to see KM585 (P1-8) 4
0.0010843406 ABIN2878477 IF Rabbit IgG Log in to see Polyclonal
0.0010843406 ABIN3197764 IC IF WB Rabbit Log in to see Polyclonal
0.0010843406 ABIN2333259 FACS IF IP IHC Mouse IgG2b kappa Log in to see dG9
0.0010843406 ABIN4344819 FACS ICC IF IHC IHC (fro) IHC (p) IP DyLight 350 Mouse IgG2b kappa Log in to see dG9
0.0010843406 ABIN4344821 FACS ICC IF IHC IHC (fro) IHC (p) IP DyLight 405 Mouse IgG2b kappa Log in to see dG9
0.0010843406 ABIN4344823 FACS ICC IF IHC IHC (fro) IHC (p) IP PE Mouse IgG2b kappa Log in to see dG9
0.0010843406 ABIN4344825 FACS ICC IF IHC IHC (fro) IHC (p) IP PerCP Mouse IgG2b kappa Log in to see dG9
0.0010843406 ABIN4344827 FACS ICC IF IHC IHC (fro) IHC (p) IP Allophycocyanin Mouse IgG2b kappa Log in to see dG9
0.0010843406 ABIN4344829 FACS ICC IF IHC IHC (fro) IHC (p) IP DyLight 755 Mouse IgG2b kappa Log in to see dG9
0.0010843406 ABIN4344831 FACS ICC IF IHC IHC (fro) IHC (p) IP DyLight 488 Mouse IgG2b kappa Log in to see dG9
0.0010843406 ABIN4344833 FACS ICC IF IHC IHC (fro) IHC (p) IP DyLight 405LS Mouse IgG2b kappa Log in to see dG9
0.0010843406 ABIN4344835 FACS ICC IF IHC IHC (fro) IHC (p) IP DyLight 680 Mouse IgG2b kappa Log in to see dG9
0.0010843406 ABIN4344837 FACS ICC IF IHC IHC (fro) IHC (p) IP Alexa Fluor 405 Mouse IgG2b kappa Log in to see dG9
0.0010843406 ABIN4344839 FACS ICC IF IHC IHC (fro) IHC (p) IP Alexa Fluor 488 Mouse IgG2b kappa Log in to see dG9

Top referenced anti-Perforin 1 antibody for Immunofluorescence

  • Human Monoclonal Perforin 1 Primary Antibody for FACS, IF - ABIN222977 (1 Publications): Dulphy, Haas, Busson, Belhadj, Peffault de Latour, Robin, Carmagnat, Loiseau, Tamouza, Scieux, Rabian, Di Santo, Charron, Janin, Socié, Toubert: An unusual CD56(bright) CD16(low) NK cell subset dominates the early posttransplant period following HLA-matched hematopoietic stem cell transplantation. in Journal of immunology (Baltimore, Md. : 1950) 2008 (PubMed)

  • Human Monoclonal Perforin 1 Primary Antibody for EIA, FACS - ABIN187239 (4 Publications): Araki, Fann, Wersto, Weng: Histone acetylation facilitates rapid and robust memory CD8 T cell response through differential expression of effector molecules (eomesodermin and its targets: perforin and granzyme B). in Journal of immunology (Baltimore, Md. : 1950) 2008 (PubMed)

Similar anti-Perforin 1 Antibodies

Application / Reactivity Mouse (Murine) Human
ELISA 6 Antibodies 23 Antibodies
Enzyme Immunoassay (EIA) 1 Antibodies
Flow Cytometry (FACS) 5 Antibodies
Immunochromatography (IC) 4 Antibodies
Immunocytochemistry (ICC) 20 Antibodies
Immunofluorescence (IF) 21 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 1 Antibodies
Immunohistochemistry (IHC) 21 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 18 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 18 Antibodies
Immunoprecipitation (IP) 22 Antibodies
Intracellular Staining (ICS) 3 Antibodies
Western Blotting (WB) 36 Antibodies
ELISA (Capture) 2 Antibodies
ELISA (Detection) 2 Antibodies
ELISpot 6 Antibodies
ELISpot (Capture) (ELISPOT (Capture)) 1 Antibodies

General

Antigen Perforin 1 (Pore Forming Protein) (PRF1) Antibodies
Reactivity Human, Mouse (Murine)
(161), (47), (21), (18), (1), (1)
Host Rabbit
(108), (71), (28)
Clonality
Polyclonal
Conjugate This Perforin 1 antibody is un-conjugated
(18), (13), (10), (5), (4), (4), (4), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1)
Application Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)
(91), (79), (61), (59), (44), (44), (44), (42), (31), (9), (6), (6), (3), (2), (2), (1), (1), (1), (1), (1)
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Product Details anti-Perforin 1 Antibody

Target Details Perforin 1 Application Details Handling Images
Purification Antigen affinity purification
Immunogen Recombinant protein of human PRF1
Isotype IgG

Target Details Perforin 1

Product Details anti-Perforin 1 Antibody Application Details Handling Images back to top
Antigen
Alternative Name PRF1 (PRF1 Antibody Abstract)
Background Granzymes are a family of serine proteases expressed by cytotoxic T lymphocytes and natural killer (NK) cells and are key components of immune responses to pathogens and transformed cells (1). Granzymes are synthesized as zymogens and are processed into mature enzymes by cleavage of a leader sequence. They are released by exocytosis in lysosome-like granules containing perforin, a membrane pore-forming protein. Granzyme B has the strongest apoptotic activity of all the granzymes as a result of its caspase-like ability to cleave substrates at aspartic acid residues thereby activating procaspases directly and cleaving downstream caspase substrates (2,3). Perforin is a pore-forming protein that facilitates the entry of cytotoxic serine proteases, such as granzymes, into target cells (4). Perforin is primarily expressed in cytotoxic T lymphocytes and NK cells.
Synonyms: PRF1,FLH2,HPLH2,MGC65093,P1,PFN1,PFP
Molecular Weight Observed MW: 61kDa
Pathways Apoptosis, Caspase Cascade in Apoptosis

Application Details

Product Details anti-Perforin 1 Antibody Target Details Perforin 1 Handling Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-Perforin 1 Antibody Target Details Perforin 1 Application Details Images back to top
Format Liquid
Handling Advice Avoid freeze / thaw cycles.
Storage -20 °C/-80 °C
Storage Comment Store at -20°C (regular) and -80°C (long term).

Images

Product Details anti-Perforin 1 Antibody Target Details Perforin 1 Application Details Handling back to top
Supplier Images
Immunofluorescence (IF) image for anti-Perforin 1 antibody (Perforin 1 (Pore Forming Protein)) (ABIN2403408) Immunofluorescence analysis analysis of HeLa cell using PRF1 antibody. Blue: DAPI for...
Immunohistochemistry (IHC) image for anti-Perforin 1 antibody (Perforin 1 (Pore Forming Protein)) (ABIN2403408) Immunohistochemistry of paraffin-embedded human lung cancer using PRF1 antibody at di...
Immunohistochemistry (IHC) image for anti-Perforin 1 antibody (Perforin 1 (Pore Forming Protein)) (ABIN2403408) Immunohistochemistry of paraffin-embedded human kidney cancer using PRF1 antibody at ...
Western Blotting (WB) image for anti-Perforin 1 antibody (Perforin 1 (Pore Forming Protein)) (ABIN2403408) Western blot analysis of extracts of various cell lines, using PRF1 antibody.