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anti-Mouse (Murine) TNFRSF12A antibody for Immunohistochemistry (Frozen Sections)

Recommended TNFRSF12A Antibody (supplied by: Log in to see )

Antigen
Tumor Necrosis Factor Receptor Superfamily, Member 12A (TNFRSF12A) Antibodies
  • AI255180
  • C87282
  • CD266
  • Fn14
  • FN14
  • HPIP
  • TWEAK-R
  • TweakR
  • TWEAKR
Reactivity
Human, Mouse (Murine)
95
43
23
Host
Mouse
49
47
2
Clonality
Monoclonal
Conjugate
This TNFRSF12A antibody is un-conjugated
11
6
5
2
2
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB)
51
47
19
17
15
14
11
10
3
3
2
1
1
1
1
Supplier
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Available images

Catalog No. ABIN281763
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.000883884 ABIN2479564 IHC (fro) FACS WB Mouse IgG2b Log in to see ITEM-4 1
0.000883884 ABIN3199692 FACS Func IHC (fro) WB PE Mouse IgG2b Log in to see ITEM-4
0.000883884 ABIN2678252 Func IHC (fro) FACS WB Mouse IgG2b kappa Log in to see ITEM-4
0.000883884 ABIN3199690 FACS Func IHC (fro) WB Mouse IgG2b Log in to see ITEM-4
0.000883884 ABIN3199691 FACS Func IHC (fro) WB Mouse IgG2b Log in to see ITEM-4

Top referenced anti-TNFRSF12A antibody for Immunohistochemistry (Frozen Sections)

Similar anti-TNFRSF12A Antibodies

Application / Reactivity Mouse (Murine) Human
ELISA 6 Antibodies 16 Antibodies
Flow Cytometry (FACS) 23 Antibodies 46 Antibodies
Functional Studies (Func) 11 Antibodies 14 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies 11 Antibodies
Immunohistochemistry (IHC) 7 Antibodies 18 Antibodies
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) 3 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 6 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 3 Antibodies
Mass Cytometry (CyTOF) 1 Antibodies
Western Blotting (WB) 20 Antibodies
Blocking Reagent (BR) 1 Antibodies
Enzyme Immunoassay (EIA) 1 Antibodies
Immunofluorescence (IF) 2 Antibodies
Immunohistochemistry (Acetone-fixed) (IHC (af)) 3 Antibodies

General

Antigen Tumor Necrosis Factor Receptor Superfamily, Member 12A (TNFRSF12A) Antibodies
Reactivity Human, Mouse (Murine)
(95), (43), (23)
Host Mouse
(49), (47), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This TNFRSF12A antibody is un-conjugated
(11), (6), (5), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB)
(51), (47), (19), (17), (15), (14), (11), (10), (3), (3), (2), (1), (1), (1), (1)
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Product Details anti-TNFRSF12A Antibody

Target Details TNFRSF12A Application Details Handling Images
Specificity The ITEM-4 antibody reacts with human TWEAK Receptor/Fn14 (fibroblast growth factor-inducible 14 kDa protein). Fn14 is distantly related to the TNFR family, containing one cysteine-rich domain in the extracellular region and a TNFR-associated factor binding domain but does not contain a death domain (DD) cytoplasmic region. Fn14 plays a role in TWEAK-induced endothelial cell migration, proliferation, and angiogenesis. TWEAK-induced cell death via Fn14 includes both apoptosis and necrosis and can be blocked by an anti-TWEAK antibody, CARL-1. Fn14 is expressed on HUVEC and in some cancer tissues but not on freshly isolated PBMCs. Fn14 mRNA expression has been identified during liver regeneration. It has been reported that ITEM-4 cross-reacts with mouse Fn14. In the mouse, Fn14 is reported to be constitutively expressed by Colon 26 cell line.
The ITEM-4 antibody has been show to have blocking function.
Characteristics The ITEM-4 antibody reacts with human TWEAK Receptor/Fn14 (fibroblast growth factor-inducible 14 kDa protein). Fn14 is distantly related to the TNFR family, containing one cysteine-rich domain in the extracellular region and a TNFR-associated factor binding domain but does not contain a death domain (DD) cytoplasmic region. Fn14 plays a role in TWEAK-induced endothelial cell migration, proliferation, and angiogenesis. TWEAK-induced cell death via Fn14 includes both apoptosis and necrosis and can be blocked by an anti-TWEAK antibody, CARL-1. Fn14 is expressed on HUVEC and in some cancer tissues but not on freshly isolated PBMCs. Fn14 mRNA expression has been identified during liver regeneration. It has been reported that ITEM-4 cross-reacts with mouse Fn14. In the mouse, Fn14 is reported to be constitutively expressed by Colon 26 cell line. The ITEM-4 antibody has been show to have blocking function.
Synonyms: Synonyms: TNFRSF12A , TWEAKR, Fibroblast growth factor-inducible 14, Fn14
Purification Affinity Purified
Clone ITEM-4
Isotype IgG2b kappa

Target Details TNFRSF12A

Product Details anti-TNFRSF12A Antibody Application Details Handling Images back to top
Antigen
Alternative Name Fn14 (TNFRSF12A Antibody Abstract)
Background Fn14 is distantly related to the TNFR family, containing one cysteine-rich domain in the extracellular region and a TNFR-associated factor binding domain but does not contain a death domain (DD) cytoplasmic region. Fn14 plays a role in TWEAK-induced endothelial cell migration, proliferation, and angiogenesis. TWEAK-induced cell death via Fn14 includes both apoptosis and necrosis and can be blocked by an anti-TWEAK antibody, CARL-1. Fn14 is expressed on HUVEC and in some cancer tissues but not on freshly isolated PBMCs. Fn14 mRNA expression has been identified during liver regeneration. It has been reported that ITEM-4 cross-reacts with mouse Fn14. In the mouse, Fn14 is reported to be constitutively expressed by Colon 26 cell line.
Pathways Apoptosis

Application Details

Product Details anti-TNFRSF12A Antibody Target Details TNFRSF12A Handling Images back to top
Application Notes Applications reported: The ITEM-4 antibody has been reported for use in flow cytometric analysis, immunoblotting (WB), and immunohistochemical staining of frozen tissue sections.

Applications tested: The ITEM-4 antibody has been tested by flow cytometric analysis on human and mouse Fn14 transfected cells. This can be used at less than or equal to 1 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 105 to 108 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Restrictions For Research Use only

Handling

Product Details anti-TNFRSF12A Antibody Target Details TNFRSF12A Application Details Images back to top
Concentration 0.5 mg/mL
Buffer aqueous buffer, 0.09 % sodium azide, may contain carrier protein/stabilizer
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Monoclonal antibodies should not be stored at a temperature below -25 °C due to the aggregation effect of the protein.
Storage 4 °C

Images

Product Details anti-TNFRSF12A Antibody Target Details TNFRSF12A Application Details Handling back to top
Supplier Images
 image for anti-TNFRSF12A antibody (Tumor Necrosis Factor Receptor Superfamily, Member 12A) (ABIN281763) Staining of non-transfected (left) and human Fn14 transfected (right) L5178Y cells wi...
 image for anti-TNFRSF12A antibody (Tumor Necrosis Factor Receptor Superfamily, Member 12A) (ABIN281763) anti-Tumor Necrosis Factor Receptor Superfamily, Member 12A (TNFRSF12A) antibody (Image 2)