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Rat (Rattus) TNFRSF12A ELISA Kit

Recommended TNFRSF12A Product (supplied by: Log in to see )

Antigen
Tumor Necrosis Factor Receptor Superfamily, Member 12A (TNFRSF12A) ELISA Kits
  • CD266
  • FN14
  • TWEAKR
  • AI255180
  • C87282
  • Fn14
  • HPIP
  • TWEAK-R
  • TweakR
  • tumor necrosis factor receptor superfamily, member 12A
  • tumor necrosis factor receptor superfamily, member 12a
  • TNFRSF12A
  • Tnfrsf12a
Reactivity
Rat (Rattus)
Alternatives
Kits with alternative reactivity to:
7
4
1
1
1
Method Type
Sandwich ELISA
Detection Range
0.3-80 ng/mL
Minimum Detection Limit
0.3 ng/mL
Application
ELISA
Options
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Catalog No. ABIN2748712
$ 556.60
Plus shipping costs $45.00

Similar TNFRSF12A ELISA Kits

Application / Reactivity Rat (Rattus)
ELISA 1 ELISA Kits

General

Antigen Tumor Necrosis Factor Receptor Superfamily, Member 12A (TNFRSF12A) ELISA Kits
Reactivity Rat (Rattus)
Kits with alternative reactivity to:
(7), (4), (1), (1), (1)
Method Type Sandwich ELISA
Detection Range 0.3-80 ng/mL
Minimum Detection Limit 0.3 ng/mL
Application ELISA
Supplier Log in to see

Product Details TNFRSF12A ELISA Kit

Target details Application Details Handling Images
Purpose Rat TWEAK R (TNFRSF12) ELISA Kit for cell culture supernatants, plasma, and serum samples.
Sample Type Plasma, Cell Culture Supernatant, Serum
Analytical Method Quantitative
Specificity Cross Reactivity: This ELISA kit shows no cross-reactivity with the following cytokines tested: rat 4-1BB, Activin A, Adiponectin, B7-1, CD48, CTACK, Decorin, Eotaxin, EphA5, Erythropoietin, FGF-BP, Fit-3 Ligand, Galectin-1, Galectin-3, Gas 1, GFR alpha-1, gp130, HGF, IL-1 R6, IL-1 R alpha, IL-2 R alpha, IL-3, IL-7, IL-17F, IL-22, JAM-A, MIP-1 alpha, Neuropilin-1, Neuropilin-2, Nope, Notch-1, Notch-2, P-Cadherin, Prolactin, RANTES, SCF, TIM-1, TIMP-2, TREM-1
Sensitivity 0.3 ng/mL
Characteristics
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data
Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Material not included
  • Distilled or deionized water
  • Precision pipettes to deliver 2 μL to 1 μL volumes
  • Adjustable 1-25 μL pipettes for reagent preparation
  • 100 μL and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis

Target details

Product Details TNFRSF12A ELISA Kit Application Details Handling Images back to top
Antigen
Alternative Name TWEAK R (TNFRSF12A ELISA Kit Abstract)
Background Tumor necrosis factor receptor superfamily, member 12a (Type 1 transmembrane protein FN14)
Gene ID 302965
UniProt Q80XX9
Pathways Apoptosis, Regulation of Cell Size

Application Details

Product Details TNFRSF12A ELISA Kit Target details Handling Images back to top
Application Notes Recommended Dilution for serum and plasma samples4 fold
Plate Pre-coated,Strips (12 x 8)
Protocol
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 μL of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4 °C.
  4. Add 100 μL of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 μL of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 μL of Stop Solution to each well.
  11. Read at 450 nm immediately.
Assay Procedure Prepare all reagents, samples and standards as instructed in the manual.Add 100 μL of standard or sample to each well.Incubate 2.5 h at RT or O/N at 4 °C.Add 100 μL of prepared biotin antibody to each well.Incubate 1 h at RT.Add 100 μL of prepared Streptavidin solution to each well.Incubate 45 min at RT.Add 100 μL of TMB One-Step Substrate Reagent to each well.Incubate 30 min at RT.Add 50 μL of Stop Solution to each well.Read at 450 nm immediately.
Restrictions For Research Use only

Handling

Product Details TNFRSF12A ELISA Kit Target details Application Details Images back to top
Storage -20 °C
Storage Comment The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Expiry Date 6 months

Images

Product Details TNFRSF12A ELISA Kit Target details Application Details Handling back to top
Supplier Images
ELISA image for TNFRSF12A ELISA Kit (Tumor Necrosis Factor Receptor Superfamily, Member 12A) (ABIN2748712) Tumor Necrosis Factor Receptor Superfamily, Member 12A (TNFRSF12A) ELISA Kit