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anti-Human YWHAQ antibody for Western Blotting

Recommended YWHAQ Antibody (supplied by: Log in to see )

Antigen
tyrosine 3-Monooxygenase/tryptophan 5-Monooxygenase Activation Protein, theta Polypeptide (YWHAQ) Antibodies
  • 1C5
  • 1c5
  • 14-3-3
  • 14-3-3t
  • 2700028P07Rik
  • AA409740
  • AU021156
  • HS1
  • hs1
  • R74690
  • ywhaq
  • ywhaq2
Epitope
AA 1-245, N-Term
17
13
9
9
8
4
4
4
4
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
139
79
78
18
17
13
11
6
5
4
3
3
3
2
2
2
2
2
2
2
1
1
1
Host
Mouse
100
35
16
9
Clonality
Monoclonal
Conjugate
This YWHAQ antibody is un-conjugated
4
3
3
2
2
2
2
2
2
2
2
2
1
1
1
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
127
52
35
28
24
23
22
5
2
1
1
Supplier
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Catalog No. ABIN336110
$ 412.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.11933539 ABIN2792144 IHC WB Rabbit N-Term Log in to see Polyclonal 2
0.11933539 ABIN264385 EIA WB Mouse IgG1 Log in to see 3B9 1
0.11933539 ABIN2855319 IHC (p) WB Rabbit IgG Center Log in to see Polyclonal
0.11933539 ABIN1534236 ELISA WB Rabbit IgG Log in to see Polyclonal
0.11933539 ABIN2688836 IF IP IHC WB Mouse IgM Log in to see 12-14-3 5
0.11933539 ABIN2688838 WB Mouse IgG1, kappa Log in to see C23 2
0.11933539 ABIN2705348 IC IF IHC WB Rabbit Center Log in to see Polyclonal
0.11933539 ABIN2465330 ELISA WB Goat Internal Region Log in to see Polyclonal
0.11933539 ABIN2463667 IHC ELISA WB Rabbit Log in to see Polyclonal
0.11933539 ABIN933751 ELISA WB Mouse IgG1 kappa AA 1-245 Log in to see AT1A1
0.11933539 ABIN1583873 IP IHC ELISA WB Rabbit N-Term Log in to see Polyclonal
0.11933539 ABIN2477178 WB Sheep IgG pSer185 Log in to see Polyclonal 1
0.11933539 ABIN1875379 IF WB Rabbit IgG Log in to see Polyclonal
0.11933539 ABIN2501268 ELISA WB Rabbit IgG Internal Region Log in to see Polyclonal
0.11933539 ABIN2501269 IF ELISA WB Rabbit IgG Ser232 Log in to see Polyclonal
0.11933539 ABIN3183083 ELISA IF IHC WB Rabbit IgG Internal Region Log in to see Polyclonal
0.11933539 ABIN926887 IHC WB Rabbit N-Term Log in to see Polyclonal
0.11933539 ABIN361138 ELISA WB Mouse IgG1, kappa chain Log in to see AT1A1
0.11933539 ABIN361482 IHC WB Rabbit pSer58 Log in to see Polyclonal 6
0.11933539 ABIN306690 WB Mouse IgG1 Log in to see 3B9 2

Top referenced anti-YWHAQ antibody for Western Blotting

Similar anti-YWHAQ Antibodies

Application / Reactivity Human
ELISA 49 Antibodies
Enzyme Immunoassay (EIA) 3 Antibodies
Flow Cytometry (FACS) 1 Antibodies
Immunochromatography (IC) 1 Antibodies
Immunocytochemistry (ICC) 8 Antibodies
Immunofluorescence (IF) 25 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 22 Antibodies
Immunohistochemistry (IHC) 35 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 23 Antibodies
Immunoprecipitation (IP) 19 Antibodies
Western Blotting (WB) 114 Antibodies

General

Antigen tyrosine 3-Monooxygenase/tryptophan 5-Monooxygenase Activation Protein, theta Polypeptide (YWHAQ) Antibodies
Epitope AA 1-245, N-Term
(17), (13), (9), (9), (8), (4), (4), (4), (4), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(139), (79), (78), (18), (17), (13), (11), (6), (5), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1)
Host Mouse
(100), (35), (16), (9)
Clonality (Clone)
Monoclonal   ( )
Conjugate This YWHAQ antibody is un-conjugated
(4), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(127), (52), (35), (28), (24), (23), (22), (5), (2), (1), (1)
Pubmed 2 references available
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Product Details anti-YWHAQ Antibody

Target Details YWHAQ Application Details Handling References for anti-YWHAQ Antibody (ABIN336110) Images
Specificity The antibody recognizes Human 14-3-3 protein theta (tau). Other species not tested.
Characteristics Synonyms: 14-3-3 protein tau, 14-3-3 protein T-cell, YWHAQ, Protein HS1
Purification Protein-G affinity chromatography
Immunogen Recombinant human 14-3-3 tau (aa 1-245) purified from E. coli
Clone AT1A1
Isotype IgG1

Target Details YWHAQ

Product Details anti-YWHAQ Antibody Application Details Handling References for anti-YWHAQ Antibody (ABIN336110) Images back to top
Antigen
Alternative Name 14-3-3 Protein theta (YWHAQ Antibody Abstract)
Background The 14-3-3 family of proteins plays a key regulatory role in signal transduction, checkpoint control, apoptotic and nutrient-sensing pathways. 14-3-3 proteins are highly conserved and ubiquitously expressed. There are at least seven isoforms, beta, gamma, epsilon, sigma, zeta, tau and eta that have been identified in mammals. The 14-3-3 tau, a subtype of the 14-3-3 family of proteins, was found in T Cells, brain and testes. This 14-3-3 tau is upregulated in patients with amyotrophic lateral sclerosis.Synonyms: 14-3-3 protein T-cell, 14-3-3 protein tau, Protein HS1, YWHAQ
Gene ID 10971
UniProt P27348
Research Area Transcription Factors
Pathways Apoptosis

Application Details

Product Details anti-YWHAQ Antibody Target Details YWHAQ Handling References for anti-YWHAQ Antibody (ABIN336110) Images back to top
Application Notes ELISA. Western blot (1/1,000-1/2,000). Immunohistochemistry on Paraffin Sections (10 μg/mL). This YWHAQ antibody was validated for use in immunohistochemistry on a panel of 21formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigenretrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides wereincubated with biotinylated secondary antibody, followed by alkalinephosphatase-streptavidin and chromogen.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-YWHAQ Antibody Target Details YWHAQ Application Details References for anti-YWHAQ Antibody (ABIN336110) Images back to top
Format Liquid
Concentration 1.0 mg/mL
Buffer PBS, pH 7.4, containing 0.09 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for up to two weeks or (in aliquots) at -20 °C or -70 °Cfor longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Expiry Date 12 months

References for anti-YWHAQ Antibody (ABIN336110)

Product Details anti-YWHAQ Antibody Target Details YWHAQ Application Details Handling Images back to top
Background publications

Liu, Elly, Yoshida, Bonnefoy-Berard, Altman: "Activation-modulated association of 14-3-3 proteins with Cbl in T cells." in: The Journal of biological chemistry, Vol. 271, Issue 24, pp. 14591-5, 1996

Xiao, Smerdon, Jones, Dodson, Soneji, Aitken, Gamblin: "Structure of a 14-3-3 protein and implications for coordination of multiple signalling pathways." in: Nature, Vol. 376, Issue 6536, pp. 188-91, 1995

Images

Product Details anti-YWHAQ Antibody Target Details YWHAQ Application Details Handling References for anti-YWHAQ Antibody (ABIN336110) back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-YWHAQ antibody (tyrosine 3-Monooxygenase/tryptophan 5-Monooxygenase Activation Protein, theta Polypeptide) (AA 1-245) (ABIN336110) anti-tyrosine 3-Monooxygenase/tryptophan 5-Monooxygenase Activation Protein, theta Polypeptide (YWHAQ) (AA 1-245), (N-Term) antibody
Immunohistochemistry (IHC) image for anti-YWHAQ antibody (tyrosine 3-Monooxygenase/tryptophan 5-Monooxygenase Activation Protein, theta Polypeptide) (AA 1-245) (ABIN336110) anti-tyrosine 3-Monooxygenase/tryptophan 5-Monooxygenase Activation Protein, theta Polypeptide (YWHAQ) (AA 1-245), (N-Term) antibody (Image 2)
Western Blotting (WB) image for anti-YWHAQ antibody (tyrosine 3-Monooxygenase/tryptophan 5-Monooxygenase Activation Protein, theta Polypeptide) (AA 1-245) (ABIN336110) anti-tyrosine 3-Monooxygenase/tryptophan 5-Monooxygenase Activation Protein, theta Polypeptide (YWHAQ) (AA 1-245), (N-Term) antibody (Image 3)