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anti-Human YWHAZ antibody for Enzyme Immunoassay

Recommended YWHAZ Antibody (supplied by: Log in to see )

Antigen
tyrosine 3-Monooxygenase/tryptophan 5-Monooxygenase Activation Protein, zeta Polypeptide (YWHAZ) Antibodies
  • 2G1
  • 4-3-3 zeta
  • 5.11
  • 14-3-3
  • 14-3-3 zeta
  • 14-3-3-zeta
  • 14-3-3-zetaisoform
  • 14-3-3leo
  • 14-3-3z
  • 14-3-3zeta
  • 14-3-3ZETA
  • 549
  • 1433z
  • 1110013I11Rik
  • 1300003C17Rik
  • ACYPI003154
  • AI596267
  • AL022924
  • AU020854
  • BEST:GH05075
  • CG17870
  • D14-3-3
  • D14-3-3zeta
  • d14-3-3zeta
  • Dmel\\CG17870
  • fb14h09
  • K
  • kcip-1
  • KCIP-1
  • l(2)07103
  • l(2)46CFe
  • l(2)46Ee
  • LEO
  • Leo
  • leo
  • par-5
  • Par-5
  • PAR-5
  • PAR5
  • Pkcz
  • r14-3-3
  • wu:fb05g08
  • wu:fb14h09
  • YWHAD
  • ywhai
  • ywhaq
  • ywhaz
  • Ywhaz
  • ywhaza
  • ywhazb
  • zgc:55807
Epitope
Thr232
46
17
12
7
6
5
5
5
4
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
171
91
82
15
14
13
11
5
3
2
2
1
1
Host
Rabbit
164
10
1
Clonality
Polyclonal
Conjugate
This YWHAZ antibody is un-conjugated
2
2
1
1
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
138
73
50
45
39
11
11
11
3
3
2
Supplier
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Catalog No. ABIN360667
$ 390.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008833851 ABIN318724 EIA IHC (p) WB Rabbit IgG N-Term, acetylated Log in to see Polyclonal 2
0.0008833851 ABIN1105137 EIA IHC (p) WB Rabbit pSer184 Log in to see Polyclonal

Top referenced anti-YWHAZ antibody for Enzyme Immunoassay

Similar anti-YWHAZ Antibodies

Application / Reactivity Human
ELISA 46 Antibodies
Enzyme Immunoassay (EIA) 3 Antibodies
Flow Cytometry (FACS) 3 Antibodies
Immunochromatography (IC) 3 Antibodies
Immunocytochemistry (ICC) 14 Antibodies
Immunofluorescence (IF) 48 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies
Immunohistochemistry (IHC) 76 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 41 Antibodies
Immunoprecipitation (IP) 12 Antibodies
Western Blotting (WB) 139 Antibodies

General

Antigen tyrosine 3-Monooxygenase/tryptophan 5-Monooxygenase Activation Protein, zeta Polypeptide (YWHAZ) Antibodies
Epitope Thr232
(46), (17), (12), (7), (6), (5), (5), (5), (4), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(171), (91), (82), (15), (14), (13), (11), (5), (3), (2), (2), (1), (1)
Host Rabbit
(164), (10), (1)
Clonality
Polyclonal
Conjugate This YWHAZ antibody is un-conjugated
(2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(138), (73), (50), (45), (39), (11), (11), (11), (3), (3), (2)
Pubmed 4 references available
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Product Details anti-YWHAZ Antibody

Target Details YWHAZ Application Details Handling References for anti-YWHAZ Antibody (ABIN360667) Images
Specificity This antibody reacts to 14-3-3 protein zeta/delta.
Purification Protein A column, followed by peptide affinity purification
Immunogen This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide corresponding to amino acid residues surrounding T232 of human 14-3-3.
Isotype Ig

Target Details YWHAZ

Product Details anti-YWHAZ Antibody Application Details Handling References for anti-YWHAZ Antibody (ABIN360667) Images back to top
Antigen
Alternative Name 14-3-3 Protein zeta/delta (YWHAZ Antibody Abstract)
Background 14-3-3 protein zeta/delta belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This protein interacts with IRS1 protein, suggesting a role in regulating insulin sensitivity. This highly conserved protein family is found in both plants and mammals, and this protein is 99 % identical to the mouse, rat and sheep orthologs.Synonyms: KCIP-1, Protein kinase C inhibitor protein 1, YWHAZ
Gene ID 7534, 9606
UniProt P63104
Research Area Transcription Factors
Pathways Apoptosis

Application Details

Product Details anti-YWHAZ Antibody Target Details YWHAZ Handling References for anti-YWHAZ Antibody (ABIN360667) Images back to top
Application Notes ELISA: 1/1,000. Western blotting: 1/50 - 1/100. Immunohistochemistry: 1/10 - 1/50.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-YWHAZ Antibody Target Details YWHAZ Application Details References for anti-YWHAZ Antibody (ABIN360667) Images back to top
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

References for anti-YWHAZ Antibody (ABIN360667)

Product Details anti-YWHAZ Antibody Target Details YWHAZ Application Details Handling Images back to top
Background publications

Li, Mofunanya, Harris, Takemaru: "Chibby cooperates with 14-3-3 to regulate beta-catenin subcellular distribution and signaling activity." in: The Journal of cell biology, Vol. 181, Issue 7, pp. 1141-54, 2008

Mateo, Llorca, Infante, Rodríguez-Rodríguez, Berciano, Combarros: "Gene-gene interaction between 14-3-3 zeta and butyrylcholinesterase modulates Alzheimer's disease risk." in: European journal of neurology : the official journal of the European Federation of Neurological Societies, Vol. 15, Issue 3, pp. 219-22, 2008

Li, Zhao, Du, Park, Sun, Bernal-Mizrachi, Aitken, Khuri, Fu: "Down-regulation of 14-3-3zeta suppresses anchorage-independent growth of lung cancer cells through anoikis activation." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 105, Issue 1, pp. 162-7, 2008

Powell, Rane, Joughin, Kalmukova, Hong, Tidor, Dean, Pierce, Klein, Yaffe, McLeish: "Proteomic identification of 14-3-3zeta as a mitogen-activated protein kinase-activated protein kinase 2 substrate: role in dimer formation and ligand binding." in: Molecular and cellular biology, Vol. 23, Issue 15, pp. 5376-87, 2003

Images

Product Details anti-YWHAZ Antibody Target Details YWHAZ Application Details Handling References for anti-YWHAZ Antibody (ABIN360667) back to top
Supplier Images
 image for anti-YWHAZ antibody (tyrosine 3-Monooxygenase/tryptophan 5-Monooxygenase Activation Protein, zeta Polypeptide) (Thr232) (ABIN360667) anti-tyrosine 3-Monooxygenase/tryptophan 5-Monooxygenase Activation Protein, zeta Polypeptide (YWHAZ) (Thr232) antibody
 image for anti-YWHAZ antibody (tyrosine 3-Monooxygenase/tryptophan 5-Monooxygenase Activation Protein, zeta Polypeptide) (Thr232) (ABIN360667) anti-tyrosine 3-Monooxygenase/tryptophan 5-Monooxygenase Activation Protein, zeta Polypeptide (YWHAZ) (Thr232) antibody (Image 2)